BRENDA - Enzyme Database

The purification and properties of a triacetic acid-hydrolyzing enzyme

Connors, W.M.; Stotz, E.; J. Biol. Chem. 178, 881-890 (1949)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.7.1.2
Benzoylacetone
9 mM
Bos taurus
3.7.1.2
citrate
9 mM
Bos taurus
3.7.1.2
Fe3+
inactivation by precipitating the proteins
Bos taurus
3.7.1.2
La3+
inactivation by precipitating the proteins
Bos taurus
3.7.1.2
malonate
9 mM
Bos taurus
3.7.1.2
oxalate
9 mM
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.7.1.2
1.38
-
triacetic acid
-
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.7.1.2
Cu2+
-
Bos taurus
3.7.1.2
Mg2+
-
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.7.1.2
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.7.1.2
liver
-
Bos taurus
-
Storage Stability
EC Number
Storage Stability
Organism
3.7.1.2
4°C, 1 month
Bos taurus
3.7.1.2
frozen, 3 months
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.7.1.2
2,4-diketohexanoate + H2O
-
289239
Bos taurus
pyruvate + propanoate
-
289239
Bos taurus
?
3.7.1.2
triacetic acid + H2O
-
289239
Bos taurus
acetoacetic acid + acetic acid
-
289239
Bos taurus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.7.1.2
6.5
-
in maleate buffer
Bos taurus
3.7.1.2
8
-
in phosphate and borate buffer
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.7.1.2
6
8.5
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.7.1.2
Benzoylacetone
9 mM
Bos taurus
3.7.1.2
citrate
9 mM
Bos taurus
3.7.1.2
Fe3+
inactivation by precipitating the proteins
Bos taurus
3.7.1.2
La3+
inactivation by precipitating the proteins
Bos taurus
3.7.1.2
malonate
9 mM
Bos taurus
3.7.1.2
oxalate
9 mM
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.7.1.2
1.38
-
triacetic acid
-
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.7.1.2
Cu2+
-
Bos taurus
3.7.1.2
Mg2+
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.7.1.2
liver
-
Bos taurus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.7.1.2
4°C, 1 month
Bos taurus
3.7.1.2
frozen, 3 months
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.7.1.2
2,4-diketohexanoate + H2O
-
289239
Bos taurus
pyruvate + propanoate
-
289239
Bos taurus
?
3.7.1.2
triacetic acid + H2O
-
289239
Bos taurus
acetoacetic acid + acetic acid
-
289239
Bos taurus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.7.1.2
6.5
-
in maleate buffer
Bos taurus
3.7.1.2
8
-
in phosphate and borate buffer
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.7.1.2
6
8.5
-
Bos taurus