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Literature summary extracted from

  • Yamada, H.; Shimizu, S.; Kim, J.M.; Shinmen, Y.; Sakai, T.
    A novel metabolic pathway for creatinine degradation in Pseudomonas putida 77 (1985), FEMS Microbiol. Lett., 30, 337-340.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.3 sarcosine + acceptor + H2O Pseudomonas putida acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate glycine + formaldehyde + reduced acceptor
-
 ?
3.5.4.21 creatinine + H2O Pseudomonas putida 77
-
 N-methylhydantoin + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.3 Pseudomonas putida
-
-
-
3.5.4.21 Pseudomonas putida 77
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.21 0.00015
-
 N-methylhydantoin added to culture medium Pseudomonas putida 77
3.5.4.21 0.0452
-
-
 Pseudomonas putida 77

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.3 sarcosine + acceptor + H2O acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate Pseudomonas putida glycine + formaldehyde + reduced acceptor
-
 ?
3.5.4.21 creatinine + H2O
-
 Pseudomonas putida 77 N-methylhydantoin + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.21 37
-
 assay at Pseudomonas putida 77