BRENDA - Enzyme Database

Properties of an affinity-column-purified human deoxycytidylate deaminase

Maley, G.F.; Lobo, A.P.; Maley, F.; Biochim. Biophys. Acta 1162, 161-170 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
activation by dCTP is reversed by addition of dTTP; allosteric activator; positive allosteric effector
Homo sapiens
3.5.4.12
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.12
cDNA from HeLa cells can be expressed in an active form
Homo sapiens
General Stability
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Homo sapiens
3.5.4.12
dCTP and MgCl2 stabilize
Homo sapiens
3.5.4.12
enzyme extremely labile, stabilized by dCTP /Mg2+
Homo sapiens
3.5.4.12
ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol
Homo sapiens
3.5.4.12
ethyleneglycol, 10-20% stabilizes
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate
competitive inhibition, Ki: 0.000012 mM
Homo sapiens
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
potent inhibitor, Ki: 0.00001 mM
Homo sapiens
3.5.4.12
5-(acryloylamino-pentanol-1)2'-beta-D-dUMP
-
Homo sapiens
3.5.4.12
5-Hg-dCMP
potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition
Homo sapiens
3.5.4.12
5-Hg-dUMP
potent inhibitor, 0.0001 mM: 54% inhibition
Homo sapiens
3.5.4.12
dAMP
-
Homo sapiens
3.5.4.12
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Homo sapiens
3.5.4.12
dGMP
-
Homo sapiens
3.5.4.12
dTTP
0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor
Homo sapiens
3.5.4.12
dUMP
-
Homo sapiens
3.5.4.12
Hg(CH3COO)2
Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
Co2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
divalent cations
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
Mg2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective; required for activation by dCTP and for inhibition by dTTP; required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Homo sapiens
3.5.4.12
Mn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
additional information
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
Zn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
20000
-
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE
Homo sapiens
3.5.4.12
117000
-
in the presence of dCTP and Mg2+, 4°C, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
dUMP + NH3
-
Homo sapiens
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Homo sapiens
-
human
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
HeLa cells
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; most dramatic allosteric response at substrate levels 0.1 mM dCMP or less; regulation, allosteric end-product regulation
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
HeLa cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
481
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
-20°C, 10-20% ethyleneglycol, 0.01 mM dCTP, 1 mM MgCl2, 20 mM 2-mercaptoethanol and 0.05% Triton X-100, 1 year, no loss in activity
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-Hg-dCMP + H2O
very poor substrate in the absence of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate
289018
Homo sapiens
UMP + NH3
-
-
-
-
3.5.4.12
dCMP + H2O
most dramatic allosteric response at substrate levels 0.1 mM dCMP or less
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
3.5.4.12
dCMP + H2O
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
3.5.4.12
additional information
substrate specificity
289018
Homo sapiens
?
-
-
-
-
3.5.4.12
additional information
HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+
289018
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE; 6 identical subunits
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
30
-
optimum condition
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
960
-
dCMP
-
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
8.5
HeLa cells, in presence or absence of dCTP and Mg2+
Homo sapiens
3.5.4.12
8
-
assay at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
activation by dCTP is reversed by addition of dTTP; allosteric activator; positive allosteric effector
Homo sapiens
3.5.4.12
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
cDNA from HeLa cells can be expressed in an active form
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Homo sapiens
3.5.4.12
dCTP and MgCl2 stabilize
Homo sapiens
3.5.4.12
enzyme extremely labile, stabilized by dCTP /Mg2+
Homo sapiens
3.5.4.12
ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol
Homo sapiens
3.5.4.12
ethyleneglycol, 10-20% stabilizes
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate
competitive inhibition, Ki: 0.000012 mM
Homo sapiens
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
potent inhibitor, Ki: 0.00001 mM
Homo sapiens
3.5.4.12
5-(acryloylamino-pentanol-1)2'-beta-D-dUMP
-
Homo sapiens
3.5.4.12
5-Hg-dCMP
potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition
Homo sapiens
3.5.4.12
5-Hg-dUMP
potent inhibitor, 0.0001 mM: 54% inhibition
Homo sapiens
3.5.4.12
dAMP
-
Homo sapiens
3.5.4.12
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Homo sapiens
3.5.4.12
dGMP
-
Homo sapiens
3.5.4.12
dTTP
0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor
Homo sapiens
3.5.4.12
dUMP
-
Homo sapiens
3.5.4.12
Hg(CH3COO)2
Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
Co2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
divalent cations
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
Mg2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective; required for activation by dCTP and for inhibition by dTTP; required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Homo sapiens
3.5.4.12
Mn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
additional information
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
3.5.4.12
Zn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
20000
-
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE
Homo sapiens
3.5.4.12
117000
-
in the presence of dCTP and Mg2+, 4°C, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
dUMP + NH3
-
Homo sapiens
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
HeLa cells
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
HeLa cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
481
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
-20°C, 10-20% ethyleneglycol, 0.01 mM dCTP, 1 mM MgCl2, 20 mM 2-mercaptoethanol and 0.05% Triton X-100, 1 year, no loss in activity
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-Hg-dCMP + H2O
very poor substrate in the absence of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate
289018
Homo sapiens
UMP + NH3
-
-
-
-
3.5.4.12
dCMP + H2O
most dramatic allosteric response at substrate levels 0.1 mM dCMP or less
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
3.5.4.12
dCMP + H2O
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
3.5.4.12
additional information
substrate specificity
289018
Homo sapiens
?
-
-
-
-
3.5.4.12
additional information
HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+
289018
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE; 6 identical subunits
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
30
-
optimum condition
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
960
-
dCMP
-
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
8.5
HeLa cells, in presence or absence of dCTP and Mg2+
Homo sapiens
3.5.4.12
8
-
assay at
Homo sapiens