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Literature summary extracted from

  • Maley, G.F.; Lobo, A.P.; Maley, F.
    Properties of an affinity-column-purified human deoxycytidylate deaminase (1993), Biochim. Biophys. Acta, 1162, 161-170.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 dCTP allosteric activator Homo sapiens
3.5.4.12 dCTP positive allosteric effector Homo sapiens
3.5.4.12 dCTP activation by dCTP is reversed by addition of dTTP Homo sapiens
3.5.4.12 dTTP allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.12 cDNA from HeLa cells can be expressed in an active form Homo sapiens

General Stability

EC Number General Stability Organism
3.5.4.12 2-mercaptoethanol stabilizes Homo sapiens
3.5.4.12 dCTP and MgCl2 stabilize Homo sapiens
3.5.4.12 enzyme extremely labile, stabilized by dCTP /Mg2+ Homo sapiens
3.5.4.12 ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol Homo sapiens
3.5.4.12 ethyleneglycol, 10-20% stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate competitive inhibition, Ki: 0.000012 mM Homo sapiens
3.5.4.12 3,4,5,6-tetrahydro-2'-dUMP potent inhibitor, Ki: 0.00001 mM Homo sapiens
3.5.4.12 5-(acryloylamino-pentanol-1)2'-beta-D-dUMP
-
Homo sapiens
3.5.4.12 5-Hg-dCMP potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition Homo sapiens
3.5.4.12 5-Hg-dUMP potent inhibitor, 0.0001 mM: 54% inhibition Homo sapiens
3.5.4.12 dAMP
-
Homo sapiens
3.5.4.12 dCTP allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH Homo sapiens
3.5.4.12 dGMP
-
Homo sapiens
3.5.4.12 dTTP 0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor Homo sapiens
3.5.4.12 dUMP
-
Homo sapiens
3.5.4.12 Hg(CH3COO)2 Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.12 Ca2+ divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective Homo sapiens
3.5.4.12 Co2+ divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective Homo sapiens
3.5.4.12 divalent cations divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective Homo sapiens
3.5.4.12 Mg2+ divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective Homo sapiens
3.5.4.12 Mg2+ required for activation by dCTP and for inhibition by dTTP Homo sapiens
3.5.4.12 Mg2+ required for activation by dCTP, Mg2+ in the absence of dCTP: no effect Homo sapiens
3.5.4.12 Mn2+ divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective Homo sapiens
3.5.4.12 additional information divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective Homo sapiens
3.5.4.12 Zn2+ divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.12 20000
-
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence Homo sapiens
3.5.4.12 20000
-
6 * 20000, SDS-PAGE Homo sapiens
3.5.4.12 117000
-
in the presence of dCTP and Mg2+, 4°C, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Homo sapiens enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division dUMP + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Homo sapiens
-
human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12 HeLa cells Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Homo sapiens
3.5.4.12 dCMP + H2O = dUMP + NH3 most dramatic allosteric response at substrate levels 0.1 mM dCMP or less Homo sapiens
3.5.4.12 dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.12 HeLa cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.12 481
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.5.4.12 -20°C, 10-20% ethyleneglycol, 0.01 mM dCTP, 1 mM MgCl2, 20 mM 2-mercaptoethanol and 0.05% Triton X-100, 1 year, no loss in activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 5-Hg-dCMP + H2O very poor substrate in the absence of mercaptoethanol Homo sapiens 5-Hg-dUMP + NH3
-
?
3.5.4.12 5-Hg-dCMP + H2O deamination activated by dTTP and inhibited by dCTP Homo sapiens 5-Hg-dUMP + NH3
-
?
3.5.4.12 5-Hg-dCMP + H2O 5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol Homo sapiens 5-Hg-dUMP + NH3
-
?
3.5.4.12 5-hydroxymethyl-dCMP + H2O HeLa cells, good substrate Homo sapiens 5-hydroxymethyl-dUMP + NH3
-
?
3.5.4.12 5-methyl-dCMP + H2O HeLa cells, good substrate Homo sapiens 5-methyl-dUMP + NH3
-
?
3.5.4.12 CMP + H2O HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate Homo sapiens UMP + NH3
-
?
3.5.4.12 dCMP + H2O most dramatic allosteric response at substrate levels 0.1 mM dCMP or less Homo sapiens dUMP + NH3
-
?
3.5.4.12 dCMP + H2O enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division Homo sapiens dUMP + NH3
-
?
3.5.4.12 additional information substrate specificity Homo sapiens ?
-
?
3.5.4.12 additional information HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+ Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.5.4.12 hexamer 6 identical subunits Homo sapiens
3.5.4.12 hexamer 6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence Homo sapiens
3.5.4.12 hexamer 6 * 20000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.12 30
-
optimum condition Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.12 960
-
dCMP
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.12 7.5 8.5 HeLa cells, in presence or absence of dCTP and Mg2+ Homo sapiens
3.5.4.12 8
-
assay at Homo sapiens