BRENDA - Enzyme Database

Identification of a site necessary for allosteric regulation in T4-phage deoxycytidylate deaminase

Moore, J.T.; Ciesla, J.M.; Changchien, L.M.; Maley, G.F.; Maley, F.; Biochemistry 33, 2104-2112 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Escherichia virus T4
3.5.4.12
dCTP
mutant F112A, dCTP not required; positive allosteric effector; wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+
Escherichia virus T4
3.5.4.12
dCTP
positive allosteric effector
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.12
cloned and amplified in a high-expression system, expression in Escherichia coli strain BL21-DE3/pLysS
Escherichia virus T4
3.5.4.12
cloned and overexpressed in Escherichia coli strain BL21-DE3/pLysS
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
F112A
as active as wild-type even in the absence of dCTP and only weakly inhibited by dTTP, loss of allosteric regulation by dCTP and dTTP, no activation by dCTP, no inhibition by dTTP
Escherichia virus T4
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor; mutant F112A, no or very slight inhibition, high concentration, 0.24 mM: slight inhibition
Escherichia virus T4
3.5.4.12
dTTP
allosteric inhibitor
Homo sapiens
3.5.4.12
dUMP
-
Escherichia virus T4
3.5.4.12
dUMP
-
Homo sapiens
3.5.4.12
dUTP
allosteric inhibitor
Escherichia virus T4
3.5.4.12
dUTP
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Zn2+
involved in catalytic mechanism involving His104, and Cys132 and Cys135
Escherichia virus T4
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
-
dUMP + NH3
-
-
-
3.5.4.12
dCMP + H2O
Escherichia virus T4
critical branchpoint in pyrimidine deoxynucleotide metabolism
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Escherichia virus T4
-
T4-phage infected Escherichia coli; wild-type and mutant F112A
-
3.5.4.12
Homo sapiens
-
human
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
cloned wild-type and mutant F112A
Escherichia virus T4
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Escherichia virus T4
3.5.4.12
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289017
Homo sapiens
dUMP + NH3
-
-
-
-
3.5.4.12
dCMP + H2O
-
289017
Homo sapiens
dUMP + NH3
-
289017
Homo sapiens
-
3.5.4.12
dCMP + H2O
-
289017
Escherichia virus T4
dUMP + NH3
-
289017
Escherichia virus T4
?
3.5.4.12
dCMP + H2O
critical branchpoint in pyrimidine deoxynucleotide metabolism
289017
Escherichia virus T4
dUMP + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Homo sapiens
3.5.4.12
hexamer
6 identical subunits
Escherichia virus T4
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.12
30
-
assay at
Escherichia virus T4
3.5.4.12
30
-
assay at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Escherichia virus T4
3.5.4.12
dCTP
mutant F112A, dCTP not required; positive allosteric effector; wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+
Escherichia virus T4
3.5.4.12
dCTP
positive allosteric effector
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
cloned and amplified in a high-expression system, expression in Escherichia coli strain BL21-DE3/pLysS
Escherichia virus T4
3.5.4.12
cloned and overexpressed in Escherichia coli strain BL21-DE3/pLysS
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
F112A
as active as wild-type even in the absence of dCTP and only weakly inhibited by dTTP, loss of allosteric regulation by dCTP and dTTP, no activation by dCTP, no inhibition by dTTP
Escherichia virus T4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor; mutant F112A, no or very slight inhibition, high concentration, 0.24 mM: slight inhibition
Escherichia virus T4
3.5.4.12
dTTP
allosteric inhibitor
Homo sapiens
3.5.4.12
dUMP
-
Escherichia virus T4
3.5.4.12
dUMP
-
Homo sapiens
3.5.4.12
dUTP
allosteric inhibitor
Escherichia virus T4
3.5.4.12
dUTP
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Zn2+
involved in catalytic mechanism involving His104, and Cys132 and Cys135
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
-
dUMP + NH3
-
-
-
3.5.4.12
dCMP + H2O
Escherichia virus T4
critical branchpoint in pyrimidine deoxynucleotide metabolism
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
cloned wild-type and mutant F112A
Escherichia virus T4
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289017
Homo sapiens
dUMP + NH3
-
-
-
-
3.5.4.12
dCMP + H2O
-
289017
Homo sapiens
dUMP + NH3
-
289017
Homo sapiens
-
3.5.4.12
dCMP + H2O
-
289017
Escherichia virus T4
dUMP + NH3
-
289017
Escherichia virus T4
?
3.5.4.12
dCMP + H2O
critical branchpoint in pyrimidine deoxynucleotide metabolism
289017
Escherichia virus T4
dUMP + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Homo sapiens
3.5.4.12
hexamer
6 identical subunits
Escherichia virus T4
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.12
30
-
assay at
Escherichia virus T4
3.5.4.12
30
-
assay at
Homo sapiens