BRENDA - Enzyme Database

Modulation of deoxycytidylate deaminase in intact human leukemia cells. Action of 2',2'-difluorodeoxycytidine

Xu, Y.Z.; Plunkett, W.; Biochem. Pharmacol. 44, 1819-1827 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
positive allosteric effector
Homo sapiens
Application
EC Number
Application
Commentary
Organism
3.5.4.12
pharmacology
elevated level of dCMPase in transformed cells and tumors: enzyme may represent another important target for cancer chemotherapy
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2',2'-difluoro-dCTP
dual mechanism of inhibition; partial purified enzyme
Homo sapiens
3.5.4.12
2',2'-difluorodeoxycytidine
high cellular nucleotide levels: inhibition: Concentration-dependent inhibition; inhibition by nucleotides of 2',2'-difluorodeoxycytidine, dual mechanism of inhibition
Homo sapiens
3.5.4.12
2'-deoxythymidine
0.05 mM: 60% inhibition, 0.01 mM: 5% inhibition; dThd
Homo sapiens
3.5.4.12
3,4,5,6-tetrahydro-2'-deoxyuridine
specifc inhibition
Homo sapiens
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
specific potent inhibitor
Homo sapiens
3.5.4.12
dTTP
allosteric inhibitor
Homo sapiens
3.5.4.12
additional information
little or no inhibition by 3,4,5,6-tetrahydrouridine; mechanism of inhibition
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis
dUMP + NH3
-
Homo sapiens
-
3.5.4.12
additional information
Homo sapiens
enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Homo sapiens
-
human
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
partial, leukemia cells
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
feedback regulation; mechanism; regulation, allosteric end-product regulation
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
CCRF-CEM cell
-
Homo sapiens
-
3.5.4.12
KG-1 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
2',2'-difluoro-dCMP + H2O
-
289016
Homo sapiens
2',2'-difluoro-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289016
Homo sapiens
dUMP + NH3
-
289016
Homo sapiens
-
3.5.4.12
dCMP + H2O
key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis
289016
Homo sapiens
dUMP + NH3
-
289016
Homo sapiens
-
3.5.4.12
additional information
enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells
289016
Homo sapiens
?
-
-
-
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
positive allosteric effector
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.4.12
pharmacology
elevated level of dCMPase in transformed cells and tumors: enzyme may represent another important target for cancer chemotherapy
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2',2'-difluoro-dCTP
dual mechanism of inhibition; partial purified enzyme
Homo sapiens
3.5.4.12
2',2'-difluorodeoxycytidine
high cellular nucleotide levels: inhibition: Concentration-dependent inhibition; inhibition by nucleotides of 2',2'-difluorodeoxycytidine, dual mechanism of inhibition
Homo sapiens
3.5.4.12
2'-deoxythymidine
0.05 mM: 60% inhibition, 0.01 mM: 5% inhibition; dThd
Homo sapiens
3.5.4.12
3,4,5,6-tetrahydro-2'-deoxyuridine
specifc inhibition
Homo sapiens
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
specific potent inhibitor
Homo sapiens
3.5.4.12
dTTP
allosteric inhibitor
Homo sapiens
3.5.4.12
additional information
little or no inhibition by 3,4,5,6-tetrahydrouridine; mechanism of inhibition
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis
dUMP + NH3
-
Homo sapiens
-
3.5.4.12
additional information
Homo sapiens
enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
partial, leukemia cells
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
CCRF-CEM cell
-
Homo sapiens
-
3.5.4.12
KG-1 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
2',2'-difluoro-dCMP + H2O
-
289016
Homo sapiens
2',2'-difluoro-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289016
Homo sapiens
dUMP + NH3
-
289016
Homo sapiens
-
3.5.4.12
dCMP + H2O
key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis
289016
Homo sapiens
dUMP + NH3
-
289016
Homo sapiens
-
3.5.4.12
additional information
enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells
289016
Homo sapiens
?
-
-
-
-