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Literature summary extracted from

  • Xu, Y.Z.; Plunkett, W.
    Modulation of deoxycytidylate deaminase in intact human leukemia cells. Action of 2',2'-difluorodeoxycytidine (1992), Biochem. Pharmacol., 44, 1819-1827.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.12 dCTP positive allosteric effector Homo sapiens

Application

EC Number Application Comment Organism
3.5.4.12 pharmacology elevated level of dCMPase in transformed cells and tumors: enzyme may represent another important target for cancer chemotherapy Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 2',2'-difluoro-dCTP dual mechanism of inhibition; partial purified enzyme Homo sapiens
3.5.4.12 2',2'-difluorodeoxycytidine high cellular nucleotide levels: inhibition: Concentration-dependent inhibition; inhibition by nucleotides of 2',2'-difluorodeoxycytidine, dual mechanism of inhibition Homo sapiens
3.5.4.12 2'-deoxythymidine 0.05 mM: 60% inhibition, 0.01 mM: 5% inhibition; dThd Homo sapiens
3.5.4.12 3,4,5,6-tetrahydro-2'-deoxyuridine specifc inhibition Homo sapiens
3.5.4.12 3,4,5,6-tetrahydro-2'-dUMP specific potent inhibitor Homo sapiens
3.5.4.12 dTTP allosteric inhibitor Homo sapiens
3.5.4.12 additional information little or no inhibition by 3,4,5,6-tetrahydrouridine; mechanism of inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Homo sapiens key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis dUMP + NH3
-
 ?
3.5.4.12 additional information Homo sapiens enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12 partial, leukemia cells Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.12 dCMP + H2O = dUMP + NH3 mechanism Homo sapiens
a
3.5.4.12 dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Homo sapiens
a
3.5.4.12 dCMP + H2O = dUMP + NH3 feedback regulation Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.12 CCRF-CEM cell
-
 Homo sapiens
-
3.5.4.12 KG-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 2',2'-difluoro-dCMP + H2O
-
 Homo sapiens 2',2'-difluoro-dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O
-
 Homo sapiens dUMP + NH3
-
 ?
3.5.4.12 dCMP + H2O key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis Homo sapiens dUMP + NH3
-
 ?
3.5.4.12 additional information enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells Homo sapiens ?
-
 ?