BRENDA - Enzyme Database

A T4-phage deoxycytidylate deaminase mutant that no longer requires deoxycytidine 5'-triphosphate for activation

Keefe, R.G.; Maley, G.F.; Saxl, R.L.; Maley, F.; J. Biol. Chem. 275, 12598-12602 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Enterobacteria phage T2
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Enterobacteria phage T6
3.5.4.12
5-hydroxymethyl-dCTP
natural activator, required for activity; natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Escherichia virus T4
3.5.4.12
dCTP
positive allosteric effector; severalfold activation, activation requires presence of Mg2+
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector; severalfold activation, activation requires presence of Mg2+
Enterobacteria phage T6
3.5.4.12
dCTP
hexameric form of enzyme is activated by dCTP, while the dimer is not; mutants R115E and R115Q: dCTP not required. R115Q: slight, 30-40% activation by 0.02 mM dCTP; positive allosteric effector; required for activity; severalfold activation, activation requires presence of Mg2+; wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+
Escherichia virus T4
3.5.4.12
additional information
the phage dCMP deaminase expression is increased upon infection of Escherichia coli
Escherichia virus T4
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.12
expression of wild-type and mutant enzymes in Escherichia coli
Escherichia virus T4
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
F112A
molecular weight analysis using HPLC gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, F112A varies from hexamer to dimer; site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme
Escherichia virus T4
3.5.4.12
R115E
mutant enzyme active in the absence of dCTP and Mg2+, little if any activation by dCTP, possessing turnover number or kcat that is about 15% that of wild-type enzyme, specific activity about 40-50% of wild-type enzyme. Molecular weight analysis using HPLC gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, R115E a dimer; site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme, but no longer requires deoxycytidine 5-triphosphate for activation in contrast to the wild-type enzyme
Escherichia virus T4
3.5.4.12
R115Q
mutant enzyme active in the absence of dCTP and Mg2+, possessing turnover number or kcat that is about 15% that of wild-type enzyme, specific activity about 40-50% of wild-type enzyme. Molecular weight analysis using HPLC size gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, R115Q varies from hexamer to dimer; site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme, but no longer requires deoxycytidine 5-triphosphate for activation in contrast to the wild-type enzyme
Escherichia virus T4
General Stability
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Escherichia virus T4
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
3.5.4.12
EDTA: denaturation, EDTA removes the two resident Zn2+ atoms /subunit
Escherichia virus T4
3.5.4.12
guanidine-HCl, 6 M, denaturation, complete restoration of activity on removal of denaturant by dilution or dialysis
Escherichia virus T4
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T2
3.5.4.12
dTTP
allosteric inhibitor; wild-type, 0.1 mM dTTP: 20% inhibition, mutants R115E and R115Q: 0.1 mM dTTP: no inhibition. 0.3 mM dTTP: wild-type 90% inhibition, mutants R115E and R115Q: 30% inhibition
Escherichia virus T4
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T6
3.5.4.12
EDTA
-
Escherichia virus T4
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
-
Enterobacteria phage T2
3.5.4.12
additional information
-
additional information
kinetic analysis, data and characterization of wild-type and mutants R115E, R115Q and F112A
Escherichia virus T4
3.5.4.12
0.021
-
dCMP
recombinant wild-type enzyme, pH 8.0, 30°C; wild-type, presence of dCTP and Mg2+
Escherichia virus T4
3.5.4.12
0.127
-
dCMP
R115Q, absence of dCTP; recombinant mutant R115Q, pH 8.0, 30°C
Escherichia virus T4
3.5.4.12
0.137
-
dCMP
R115E, absence of dCTP; recombinant mutant R115E, pH 8.0, 30°C
Escherichia virus T4
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP
Enterobacteria phage T2
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP
Enterobacteria phage T6
3.5.4.12
Mg2+
; required for activation by dCTP and for inhibition by dTTP
Escherichia virus T4
3.5.4.12
Zn2+
two resident Zn2+ atoms /subunit
Escherichia virus T4
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
21200
-
2 * 21200, mutant R115E: dimer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS; 6 * 21200, wild-type, particularly in the presence of dCTP: hexamer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS
Escherichia virus T4
3.5.4.12
38200
113000
recombinant mutant F112A, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
39800
118000
recombinant mutant R115Q, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
41600
45400
recombinant mutant R115E, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
44300
-
R115E, HPLC gel filtration
Escherichia virus T4
3.5.4.12
110000
122000
recombinant wild-type enzyme, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
111900
-
F112A, HPLC gel filtration
Escherichia virus T4
3.5.4.12
124500
-
R115Q, HPLC gel filtration
Escherichia virus T4
3.5.4.12
127000
-
wild-type, HPLC gel filtration
Escherichia virus T4
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Escherichia virus T4
-
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
-
dUMP + NH3
-
Enterobacteria phage T2
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Enterobacteria phage T2
-
T2-, T4-, and T6-phage induced enzyme in Escherichia coli
-
3.5.4.12
Enterobacteria phage T6
-
T2-, T4-, and T6-phage induced enzyme in Escherichia coli
-
3.5.4.12
Escherichia virus T4
-
; T2-, T4-, and T6-phage induced enzyme in Escherichia coli; T4-phage infected Escherichia coli; wild-type and R115E and R115Q, mutant enzymes that no longer requires dCTP for activation, and mutant F112A
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
recombinant wild-type and mutant enzymes from Escherichia coli; wild-type and mutants R115E, R115Q and F112A
Escherichia virus T4
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Enterobacteria phage T2
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Escherichia virus T4
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Enterobacteria phage T6
Renatured (Commentary)
EC Number
Commentary
Organism
3.5.4.12
after denaturation with EDTA, mutants R115E and R115Q restored 54% and 60% of original activities, wild-type enzyme only marginally restored; after treatment with guanidine-HCl, 6 M, complete reactivation on removal of denaturant by dilution or dialysis
Escherichia virus T4
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
mutants R115E and R115Q possessing specific activity about 40-50% of wild-type enzyme
Escherichia virus T4
3.5.4.12
90
100
mutant R115E
Escherichia virus T4
3.5.4.12
121
-
mutant R115Q
Escherichia virus T4
3.5.4.12
220
-
wild-type
Escherichia virus T4
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
-40°C, solid ammonium sulfate to 80% saturation, stable for longer periods of storage
Escherichia virus T4
3.5.4.12
0-4°C, 0.1 M 2-mercaptoethanol, stable for extended periods
Escherichia virus T4
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289015
Escherichia virus T4
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289015
Escherichia virus T4
dUMP + NH3
-
289015
Escherichia virus T4
?
3.5.4.12
dCMP + H2O
-
289015
Enterobacteria phage T2
dUMP + NH3
-
289015
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
-
289015
Enterobacteria phage T6
dUMP + NH3
-
289015
Enterobacteria phage T6
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
dimer
2 * 21200, mutant R115E: dimer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS; mutants R115Q and R115E, mutant F112A changes between dimeric and hexameric forms
Escherichia virus T4
3.5.4.12
hexamer
6 * 21200, wild-type, particularly in the presence of dCTP: hexamer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS; 6 identical subunits; wild-type enzyme, mutant F112A changes between dimeric and hexameric forms
Escherichia virus T4
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T6
3.5.4.12
More
hexameric form of enzyme is activated by dCTP, while the dimer is not
Escherichia virus T4
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
30
-
assay at
Escherichia virus T4
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
mutants R115E and R115Q possessing turnover number or kcat that is about 15% that of wild-type enzyme
Escherichia virus T4
3.5.4.12
69
-
dCMP
mutant R115E, pH 8.0, 30°C; R115E
Escherichia virus T4
3.5.4.12
85
-
dCMP
mutant R115Q, pH 8.0, 30°C; R115Q dimer
Escherichia virus T4
3.5.4.12
255
-
dCMP
R115Q hexamer
Escherichia virus T4
3.5.4.12
466
-
dCMP
wild-type; wild-type enzyme, pH 8.0, 30°C
Escherichia virus T4
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8
-
assay at
Enterobacteria phage T2
3.5.4.12
8
-
assay at
Escherichia virus T4
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Enterobacteria phage T2
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Enterobacteria phage T6
3.5.4.12
5-hydroxymethyl-dCTP
natural activator, required for activity; natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Escherichia virus T4
3.5.4.12
dCTP
positive allosteric effector; severalfold activation, activation requires presence of Mg2+
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector; severalfold activation, activation requires presence of Mg2+
Enterobacteria phage T6
3.5.4.12
dCTP
hexameric form of enzyme is activated by dCTP, while the dimer is not; mutants R115E and R115Q: dCTP not required. R115Q: slight, 30-40% activation by 0.02 mM dCTP; positive allosteric effector; required for activity; severalfold activation, activation requires presence of Mg2+; wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+
Escherichia virus T4
3.5.4.12
additional information
the phage dCMP deaminase expression is increased upon infection of Escherichia coli
Escherichia virus T4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
expression of wild-type and mutant enzymes in Escherichia coli
Escherichia virus T4
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
F112A
molecular weight analysis using HPLC gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, F112A varies from hexamer to dimer; site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme
Escherichia virus T4
3.5.4.12
R115E
mutant enzyme active in the absence of dCTP and Mg2+, little if any activation by dCTP, possessing turnover number or kcat that is about 15% that of wild-type enzyme, specific activity about 40-50% of wild-type enzyme. Molecular weight analysis using HPLC gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, R115E a dimer; site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme, but no longer requires deoxycytidine 5-triphosphate for activation in contrast to the wild-type enzyme
Escherichia virus T4
3.5.4.12
R115Q
mutant enzyme active in the absence of dCTP and Mg2+, possessing turnover number or kcat that is about 15% that of wild-type enzyme, specific activity about 40-50% of wild-type enzyme. Molecular weight analysis using HPLC size gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, R115Q varies from hexamer to dimer; site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme, but no longer requires deoxycytidine 5-triphosphate for activation in contrast to the wild-type enzyme
Escherichia virus T4
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Escherichia virus T4
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
3.5.4.12
EDTA: denaturation, EDTA removes the two resident Zn2+ atoms /subunit
Escherichia virus T4
3.5.4.12
guanidine-HCl, 6 M, denaturation, complete restoration of activity on removal of denaturant by dilution or dialysis
Escherichia virus T4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T2
3.5.4.12
dTTP
allosteric inhibitor; wild-type, 0.1 mM dTTP: 20% inhibition, mutants R115E and R115Q: 0.1 mM dTTP: no inhibition. 0.3 mM dTTP: wild-type 90% inhibition, mutants R115E and R115Q: 30% inhibition
Escherichia virus T4
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T6
3.5.4.12
EDTA
-
Escherichia virus T4
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
-
Enterobacteria phage T2
3.5.4.12
additional information
-
additional information
kinetic analysis, data and characterization of wild-type and mutants R115E, R115Q and F112A
Escherichia virus T4
3.5.4.12
0.021
-
dCMP
recombinant wild-type enzyme, pH 8.0, 30°C; wild-type, presence of dCTP and Mg2+
Escherichia virus T4
3.5.4.12
0.127
-
dCMP
R115Q, absence of dCTP; recombinant mutant R115Q, pH 8.0, 30°C
Escherichia virus T4
3.5.4.12
0.137
-
dCMP
R115E, absence of dCTP; recombinant mutant R115E, pH 8.0, 30°C
Escherichia virus T4
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP
Enterobacteria phage T2
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP
Enterobacteria phage T6
3.5.4.12
Mg2+
; required for activation by dCTP and for inhibition by dTTP
Escherichia virus T4
3.5.4.12
Zn2+
two resident Zn2+ atoms /subunit
Escherichia virus T4
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
21200
-
2 * 21200, mutant R115E: dimer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS; 6 * 21200, wild-type, particularly in the presence of dCTP: hexamer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS
Escherichia virus T4
3.5.4.12
38200
113000
recombinant mutant F112A, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
39800
118000
recombinant mutant R115Q, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
41600
45400
recombinant mutant R115E, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
44300
-
R115E, HPLC gel filtration
Escherichia virus T4
3.5.4.12
110000
122000
recombinant wild-type enzyme, gel filtration, dependent on protein concentration, in presence and absence of fdCTP
Escherichia virus T4
3.5.4.12
111900
-
F112A, HPLC gel filtration
Escherichia virus T4
3.5.4.12
124500
-
R115Q, HPLC gel filtration
Escherichia virus T4
3.5.4.12
127000
-
wild-type, HPLC gel filtration
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Escherichia virus T4
-
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
-
dUMP + NH3
-
Enterobacteria phage T2
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
recombinant wild-type and mutant enzymes from Escherichia coli; wild-type and mutants R115E, R115Q and F112A
Escherichia virus T4
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
after denaturation with EDTA, mutants R115E and R115Q restored 54% and 60% of original activities, wild-type enzyme only marginally restored; after treatment with guanidine-HCl, 6 M, complete reactivation on removal of denaturant by dilution or dialysis
Escherichia virus T4
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
mutants R115E and R115Q possessing specific activity about 40-50% of wild-type enzyme
Escherichia virus T4
3.5.4.12
90
100
mutant R115E
Escherichia virus T4
3.5.4.12
121
-
mutant R115Q
Escherichia virus T4
3.5.4.12
220
-
wild-type
Escherichia virus T4
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
-40°C, solid ammonium sulfate to 80% saturation, stable for longer periods of storage
Escherichia virus T4
3.5.4.12
0-4°C, 0.1 M 2-mercaptoethanol, stable for extended periods
Escherichia virus T4
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289015
Escherichia virus T4
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289015
Escherichia virus T4
dUMP + NH3
-
289015
Escherichia virus T4
?
3.5.4.12
dCMP + H2O
-
289015
Enterobacteria phage T2
dUMP + NH3
-
289015
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
-
289015
Enterobacteria phage T6
dUMP + NH3
-
289015
Enterobacteria phage T6
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
dimer
2 * 21200, mutant R115E: dimer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS; mutants R115Q and R115E, mutant F112A changes between dimeric and hexameric forms
Escherichia virus T4
3.5.4.12
hexamer
6 * 21200, wild-type, particularly in the presence of dCTP: hexamer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS; 6 identical subunits; wild-type enzyme, mutant F112A changes between dimeric and hexameric forms
Escherichia virus T4
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T6
3.5.4.12
More
hexameric form of enzyme is activated by dCTP, while the dimer is not
Escherichia virus T4
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
30
-
assay at
Escherichia virus T4
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
mutants R115E and R115Q possessing turnover number or kcat that is about 15% that of wild-type enzyme
Escherichia virus T4
3.5.4.12
69
-
dCMP
mutant R115E, pH 8.0, 30°C; R115E
Escherichia virus T4
3.5.4.12
85
-
dCMP
mutant R115Q, pH 8.0, 30°C; R115Q dimer
Escherichia virus T4
3.5.4.12
255
-
dCMP
R115Q hexamer
Escherichia virus T4
3.5.4.12
466
-
dCMP
wild-type; wild-type enzyme, pH 8.0, 30°C
Escherichia virus T4
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8
-
assay at
Enterobacteria phage T2
3.5.4.12
8
-
assay at
Escherichia virus T4