BRENDA - Enzyme Database

Interaction of the high-affinity inhibitor tetrahydro-dUMP with the allosteric enzyme deoxycytidylate aminohydrolase

Nucci, R.; Febbraio, F.; Piccialli, G.; de Napoli, L.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.; Arch. Biochem. Biophys. 310, 49-53 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
high-affinity inhibitor, rapid, reversible inhibition, kinetics of inhibition. At low substrate or competitor concentrations: activation. Tetrahydro-dUMP activates enzyme at low substrate concentrations
Equus asinus
3.5.4.12
dCTP
positive allosteric effector
Equus asinus
Application
EC Number
Application
Commentary
Organism
3.5.4.12
pharmacology
might have applications in cancer chemotherapy. Enzyme may be an inhibitor target for antitumor agents
Equus asinus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
3,4,5,6-tetrahydro-2'-deoxyuridine
-
Equus asinus
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
high-affinity inhibitor, rapid, reversible inhibition, kinetics of inhibition. At low substrate or competitor concentrations: activation. Tetrahydro-dUMP activates enzyme at low substrate concentrations
Equus asinus
3.5.4.12
additional information
kinetics of inhibition; mechanism of inhibition
Equus asinus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetics of inhibition
Equus asinus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Equus asinus
-
donkey
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; mechanism; regulation, allosteric end-product regulation
Equus asinus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289014
Equus asinus
dUMP + NH3
-
289014
Equus asinus
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
high-affinity inhibitor, rapid, reversible inhibition, kinetics of inhibition. At low substrate or competitor concentrations: activation. Tetrahydro-dUMP activates enzyme at low substrate concentrations
Equus asinus
3.5.4.12
dCTP
positive allosteric effector
Equus asinus
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.4.12
pharmacology
might have applications in cancer chemotherapy. Enzyme may be an inhibitor target for antitumor agents
Equus asinus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
3,4,5,6-tetrahydro-2'-deoxyuridine
-
Equus asinus
3.5.4.12
3,4,5,6-tetrahydro-2'-dUMP
high-affinity inhibitor, rapid, reversible inhibition, kinetics of inhibition. At low substrate or competitor concentrations: activation. Tetrahydro-dUMP activates enzyme at low substrate concentrations
Equus asinus
3.5.4.12
additional information
kinetics of inhibition; mechanism of inhibition
Equus asinus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetics of inhibition
Equus asinus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289014
Equus asinus
dUMP + NH3
-
289014
Equus asinus
?