BRENDA - Enzyme Database

Allosteric modifier and substrate binding of donkey deoxycytidylate aminohydrolase (EC 3.5.4.12)

Nucci, R.; Raia, C.A.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.; Arch. Biochem. Biophys. 289, 19-25 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
positive allosteric effector
Equus asinus
3.5.4.12
additional information
mechanism of activation
Equus asinus
General Stability
EC Number
General Stability
Organism
3.5.4.12
glycerol stabilizes
Equus asinus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dAMP
kinetics of dAMP inhibition
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor; cooperative inhibition, reversed by dCTP, mechanism of inhibition
Equus asinus
3.5.4.12
additional information
mechanism of inhibition
Equus asinus
3.5.4.12
UTP-adipic hydrazide
cooperative inhibition, reversed by dCTP
Equus asinus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
cooperative kinetics, kinetic affinities and effects, mechanism
Equus asinus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
18842
-
6 * 18842, amino acid analysis
Equus asinus
3.5.4.12
113100
-
amino acid analysis
Equus asinus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Equus asinus
-
donkey
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; mechanism; regulation, allosteric end-product regulation
Equus asinus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
730
-
-
Equus asinus
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
-20°C, 0.1 M Na-phosphate, pH 7.5, 50% glycerol, 12 months, no activity lost
Equus asinus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
-
289012
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289012
Equus asinus
dUMP + NH3
-
289012
Equus asinus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 18842, amino acid analysis; 6 identical subunits
Equus asinus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
38
-
assay at
Equus asinus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
1400
-
dCMP
-
Equus asinus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
positive allosteric effector
Equus asinus
3.5.4.12
additional information
mechanism of activation
Equus asinus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
glycerol stabilizes
Equus asinus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dAMP
kinetics of dAMP inhibition
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor; cooperative inhibition, reversed by dCTP, mechanism of inhibition
Equus asinus
3.5.4.12
additional information
mechanism of inhibition
Equus asinus
3.5.4.12
UTP-adipic hydrazide
cooperative inhibition, reversed by dCTP
Equus asinus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
cooperative kinetics, kinetic affinities and effects, mechanism
Equus asinus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
18842
-
6 * 18842, amino acid analysis
Equus asinus
3.5.4.12
113100
-
amino acid analysis
Equus asinus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
730
-
-
Equus asinus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
-20°C, 0.1 M Na-phosphate, pH 7.5, 50% glycerol, 12 months, no activity lost
Equus asinus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
-
289012
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289012
Equus asinus
dUMP + NH3
-
289012
Equus asinus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 18842, amino acid analysis; 6 identical subunits
Equus asinus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
38
-
assay at
Equus asinus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
1400
-
dCMP
-
Equus asinus