BRENDA - Enzyme Database

On the regulation of a bacterial deoxycytidylate deaminase

Sergott, R.C.; Debeer, L.J.; Bessman, M.J.; J. Biol. Chem. 246, 7755-7758 (1971)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
30% activation
Lactobacillus acidophilus
3.5.4.12
dCTP
positive allosteric effector; specifically markedly activated, Km: 0.00017 mM
Lactobacillus acidophilus
3.5.4.12
dCTP
positive allosteric effector
Staphylococcus aureus
3.5.4.12
dGTP
very slight activation
Lactobacillus acidophilus
General Stability
EC Number
General Stability
Organism
3.5.4.12
enzyme is unstable
Lactobacillus acidophilus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2',3'-di-dTTP
7% inhibition
Lactobacillus acidophilus
3.5.4.12
5-bromo-dUTP
92% inhibition
Lactobacillus acidophilus
3.5.4.12
dTTP
allosteric inhibitor; specifically markedly inhibited, Ki: 0.0017 mM
Lactobacillus acidophilus
3.5.4.12
dTTP
allosteric inhibitor
Staphylococcus aureus
3.5.4.12
dUTP
79% inhibition
Lactobacillus acidophilus
3.5.4.12
additional information
no inhibition by dATP, dGTP, 5-bromo-UTP
Lactobacillus acidophilus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Staphylococcus aureus
-
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Lactobacillus acidophilus
-
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Lactobacillus acidophilus
-
-
-
3.5.4.12
Staphylococcus aureus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
-
Staphylococcus aureus
3.5.4.12
partial
Lactobacillus acidophilus
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Lactobacillus acidophilus
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Staphylococcus aureus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
0.03
-
-
Lactobacillus acidophilus
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
on ice, 0.05 M Tris, pH 8.0, 20 mM dithiothreitol, stable for weeks
Lactobacillus acidophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289011
Staphylococcus aureus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289011
Staphylococcus aureus
dUMP + NH3
-
289011
Staphylococcus aureus
?
3.5.4.12
dCMP + H2O
-
289011
Lactobacillus acidophilus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289011
Lactobacillus acidophilus
dUMP + NH3
-
289011
Lactobacillus acidophilus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Lactobacillus acidophilus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
30% activation
Lactobacillus acidophilus
3.5.4.12
dCTP
positive allosteric effector; specifically markedly activated, Km: 0.00017 mM
Lactobacillus acidophilus
3.5.4.12
dCTP
positive allosteric effector
Staphylococcus aureus
3.5.4.12
dGTP
very slight activation
Lactobacillus acidophilus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
enzyme is unstable
Lactobacillus acidophilus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
2',3'-di-dTTP
7% inhibition
Lactobacillus acidophilus
3.5.4.12
5-bromo-dUTP
92% inhibition
Lactobacillus acidophilus
3.5.4.12
dTTP
allosteric inhibitor; specifically markedly inhibited, Ki: 0.0017 mM
Lactobacillus acidophilus
3.5.4.12
dTTP
allosteric inhibitor
Staphylococcus aureus
3.5.4.12
dUTP
79% inhibition
Lactobacillus acidophilus
3.5.4.12
additional information
no inhibition by dATP, dGTP, 5-bromo-UTP
Lactobacillus acidophilus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Staphylococcus aureus
-
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Lactobacillus acidophilus
-
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
-
Staphylococcus aureus
3.5.4.12
partial
Lactobacillus acidophilus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
0.03
-
-
Lactobacillus acidophilus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
on ice, 0.05 M Tris, pH 8.0, 20 mM dithiothreitol, stable for weeks
Lactobacillus acidophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289011
Staphylococcus aureus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289011
Staphylococcus aureus
dUMP + NH3
-
289011
Staphylococcus aureus
?
3.5.4.12
dCMP + H2O
-
289011
Lactobacillus acidophilus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289011
Lactobacillus acidophilus
dUMP + NH3
-
289011
Lactobacillus acidophilus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Lactobacillus acidophilus