BRENDA - Enzyme Database

T2r+ bacteriophage-induced enzymes. II. The subunit structure of deoxycytidylate deaminase

Maley, G.F.; MacColl, R.; Maley, F.; J. Biol. Chem. 247, 940-945 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP; pH-dependent activation
Enterobacteria phage T2
3.5.4.12
dCTP
activation by dCTP decreases markedly as the pH is increased, pH-dependent activation; positive allosteric effector
Enterobacteria phage T2
3.5.4.12
dCTP
pH-dependent activation; positive allosteric effector
Gallus gallus
General Stability
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
3.5.4.12
guanidine hydrochloride, 1 M, complete loss in activity accompanied by disaggregation of enzyme, 6 M: denaturation, complete reactivation with 50 mM 2-mercaptoethanol
Enterobacteria phage T2
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor
Gallus gallus
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T2
3.5.4.12
guanidine hydrochloride
0.5 M: 50% inhibition, 1 M: 100% inhibition
Enterobacteria phage T2
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
20200
-
6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE
Enterobacteria phage T2
3.5.4.12
124000
-
PAGE and sedimentation equilibrium
Enterobacteria phage T2
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Gallus gallus
-
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
-
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Enterobacteria phage T2
-
T2r+-infected Escherichia coli B
-
3.5.4.12
Gallus gallus
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Gallus gallus
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Enterobacteria phage T2
Renatured (Commentary)
EC Number
Commentary
Organism
3.5.4.12
after treatment with guanidine hydrochloride, 6 M, complete reactivation with 50 mM 2-mercaptoethanol, original hexameric structure restored
Enterobacteria phage T2
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
embryo
-
Gallus gallus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289010
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289010
Gallus gallus
dUMP + NH3
-
289010
Gallus gallus
?
3.5.4.12
dCMP + H2O
-
289010
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289010
Enterobacteria phage T2
dUMP + NH3
-
289010
Enterobacteria phage T2
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE; 6 identical subunits; hexamer is the active form
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Gallus gallus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP; pH-dependent activation
Enterobacteria phage T2
3.5.4.12
dCTP
activation by dCTP decreases markedly as the pH is increased, pH-dependent activation; positive allosteric effector
Enterobacteria phage T2
3.5.4.12
dCTP
pH-dependent activation; positive allosteric effector
Gallus gallus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
3.5.4.12
guanidine hydrochloride, 1 M, complete loss in activity accompanied by disaggregation of enzyme, 6 M: denaturation, complete reactivation with 50 mM 2-mercaptoethanol
Enterobacteria phage T2
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor
Gallus gallus
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T2
3.5.4.12
guanidine hydrochloride
0.5 M: 50% inhibition, 1 M: 100% inhibition
Enterobacteria phage T2
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
20200
-
6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE
Enterobacteria phage T2
3.5.4.12
124000
-
PAGE and sedimentation equilibrium
Enterobacteria phage T2
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Gallus gallus
-
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
-
dUMP + NH3
-
-
?
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
after treatment with guanidine hydrochloride, 6 M, complete reactivation with 50 mM 2-mercaptoethanol, original hexameric structure restored
Enterobacteria phage T2
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
embryo
-
Gallus gallus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289010
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289010
Gallus gallus
dUMP + NH3
-
289010
Gallus gallus
?
3.5.4.12
dCMP + H2O
-
289010
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289010
Enterobacteria phage T2
dUMP + NH3
-
289010
Enterobacteria phage T2
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE; 6 identical subunits; hexamer is the active form
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Gallus gallus