BRENDA - Enzyme Database

T2r+ bacteriophage-induced enzymes. I. The purification and properties of deoxycytidylate deaminase

Maley, G.F.; Guarino, D.U.; J. Biol. Chem. 247, 931-939 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP; pH-dependent activation
Escherichia virus T4
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP; pH-dependent activation
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Gallus gallus
3.5.4.12
dCTP
activation by dCTP decreases markedly as the pH is increased, pH-dependent activation; positive allosteric effector
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Escherichia virus T4
General Stability
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Escherichia virus T4
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor; inhibition by dTTP increases markedly as the pH is raised, pH-dependent inhibition
Enterobacteria phage T2
3.5.4.12
dTTP
allosteric inhibitor
Gallus gallus
3.5.4.12
dTTP
allosteric inhibitor
Escherichia virus T4
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.05
-
dCMP
pH 6.0
Enterobacteria phage T2
3.5.4.12
0.1
-
dCMP
pH 8.0
Enterobacteria phage T2
3.5.4.12
0.5
-
CMP
pH 6.0
Enterobacteria phage T2
3.5.4.12
12
-
CMP
pH 8.0, absence of effector
Enterobacteria phage T2
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
124000
-
PAGE and sedimentation equilibrium
Enterobacteria phage T2
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Gallus gallus
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Escherichia virus T4
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Enterobacteria phage T2
-
T2r+ or T4r+ bacteriophage-induced enzyme, host Escherichia coli B
-
3.5.4.12
Escherichia virus T4
-
T2r+ or T4r+ bacteriophage-induced enzyme, host Escherichia coli B
-
3.5.4.12
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
T2-phage infected Escherichia coli
Enterobacteria phage T2
3.5.4.12
T4-phage infected Escherichia coli
Escherichia virus T4
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Enterobacteria phage T2
3.5.4.12
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Escherichia virus T4
3.5.4.12
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
embryo
-
Gallus gallus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
430
-
-
Enterobacteria phage T2
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
0-4°C, neutral pH, 0.1 M 2-mercaptoethanol, stable for several months
Enterobacteria phage T2
3.5.4.12
frozen in absence of 2-mercaptoethanol, indefinitely stable
Enterobacteria phage T2
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-hydroxymethyl-dCMP + H2O
-
289009
Gallus gallus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
very poor substrate
289009
Escherichia virus T4
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
very poor substrate
289009
Enterobacteria phage T2
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
-
289009
Escherichia virus T4
UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
-
289009
Enterobacteria phage T2
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289009
Gallus gallus
dUMP + NH3
-
289009
Gallus gallus
?
3.5.4.12
dCMP + H2O
-
289009
Escherichia virus T4
dUMP + NH3
-
289009
Escherichia virus T4
?
3.5.4.12
dCMP + H2O
-
289009
Enterobacteria phage T2
dUMP + NH3
-
289009
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
289009
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
289009
Escherichia virus T4
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
289009
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Escherichia virus T4
3.5.4.12
hexamer
6 identical subunits
Gallus gallus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Enterobacteria phage T2
3.5.4.12
37
-
assay at
Escherichia virus T4
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
additional information
-
dCTP increases pH-optimum by approximately 3 pH units
Enterobacteria phage T2
3.5.4.12
additional information
-
dCTP increases pH-optimum by approximately 0.6 unit
Gallus gallus
3.5.4.12
5.3
-
in the absence of dCTP
Enterobacteria phage T2
3.5.4.12
8.3
-
in the presence of dCTP or 5-hydroxymethyl-dCTP
Enterobacteria phage T2
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP; pH-dependent activation
Escherichia virus T4
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP; pH-dependent activation
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Gallus gallus
3.5.4.12
dCTP
activation by dCTP decreases markedly as the pH is increased, pH-dependent activation; positive allosteric effector
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Escherichia virus T4
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Escherichia virus T4
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor; inhibition by dTTP increases markedly as the pH is raised, pH-dependent inhibition
Enterobacteria phage T2
3.5.4.12
dTTP
allosteric inhibitor
Gallus gallus
3.5.4.12
dTTP
allosteric inhibitor
Escherichia virus T4
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.05
-
dCMP
pH 6.0
Enterobacteria phage T2
3.5.4.12
0.1
-
dCMP
pH 8.0
Enterobacteria phage T2
3.5.4.12
0.5
-
CMP
pH 6.0
Enterobacteria phage T2
3.5.4.12
12
-
CMP
pH 8.0, absence of effector
Enterobacteria phage T2
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
124000
-
PAGE and sedimentation equilibrium
Enterobacteria phage T2
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Gallus gallus
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Escherichia virus T4
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
T2-phage infected Escherichia coli
Enterobacteria phage T2
3.5.4.12
T4-phage infected Escherichia coli
Escherichia virus T4
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
embryo
-
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
430
-
-
Enterobacteria phage T2
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
0-4°C, neutral pH, 0.1 M 2-mercaptoethanol, stable for several months
Enterobacteria phage T2
3.5.4.12
frozen in absence of 2-mercaptoethanol, indefinitely stable
Enterobacteria phage T2
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-hydroxymethyl-dCMP + H2O
-
289009
Gallus gallus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
very poor substrate
289009
Escherichia virus T4
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
very poor substrate
289009
Enterobacteria phage T2
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
-
289009
Escherichia virus T4
UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
-
289009
Enterobacteria phage T2
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289009
Gallus gallus
dUMP + NH3
-
289009
Gallus gallus
?
3.5.4.12
dCMP + H2O
-
289009
Escherichia virus T4
dUMP + NH3
-
289009
Escherichia virus T4
?
3.5.4.12
dCMP + H2O
-
289009
Enterobacteria phage T2
dUMP + NH3
-
289009
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
289009
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
289009
Escherichia virus T4
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
289009
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Escherichia virus T4
3.5.4.12
hexamer
6 identical subunits
Gallus gallus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Enterobacteria phage T2
3.5.4.12
37
-
assay at
Escherichia virus T4
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
additional information
-
dCTP increases pH-optimum by approximately 3 pH units
Enterobacteria phage T2
3.5.4.12
additional information
-
dCTP increases pH-optimum by approximately 0.6 unit
Gallus gallus
3.5.4.12
5.3
-
in the absence of dCTP
Enterobacteria phage T2
3.5.4.12
8.3
-
in the presence of dCTP or 5-hydroxymethyl-dCTP
Enterobacteria phage T2