BRENDA - Enzyme Database

Deoxycytidylate deaminase. Properties of the enzyme from cultured kidney cells of baby hamster

Rolton, H.A.; Keir, H.M.; Biochem. J. 141, 211-217 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
maximum activation at 0.04 mM, dCTP activation requires presence of Mg2+ or Mn2+; positive allosteric effector
Cricetinae
General Stability
EC Number
General Stability
Organism
3.5.4.12
dCTP and Mg2+ stabilize
Cricetinae
3.5.4.12
dCTP, 0.125 mM, or ethyleneglycol, 20% v/v, stabilize
Cricetinae
3.5.4.12
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v
Cricetinae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor; maximum inhibition at 0.015 mM, inhibition requires presence of Mg2+ or Mn2+
Cricetinae
3.5.4.12
EDTA
1 mM: 90% inhibition, restored to maximum by 1-5 mM Mg2+ or Mn2+
Cricetinae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.91
-
dCMP
-
Cricetinae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+; required for activation by dCTP and for inhibition by dTTP; required for activation by dCTP, Mg2+ in the absence of dCTP: no effect; required for complete activation by dCTP
Cricetinae
3.5.4.12
Mn2+
dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+
Cricetinae
3.5.4.12
additional information
bound bivalent metal cations probably present; metal ions not required
Cricetinae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
115000
-
BHK-21/C13 cells, sucrose density gradient centrifugation
Cricetinae
3.5.4.12
130000
-
BHK-21/C13 cells, gel filtration
Cricetinae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Cricetinae
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
dUMP + NH3
-
Cricetinae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Cricetinae
-
BHK-21/C13 cells, baby-hamster
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
partial, BHK-21/C13 cells, baby-hamster
Cricetinae
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Cricetinae
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
BHK-21 cell
baby-hamster kidney
Cricetinae
-
3.5.4.12
kidney
BHK-21/C13 cells, baby-hamster kidney cells
Cricetinae
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.4.12
0.37
-
-
Cricetinae
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
-70C
Cricetinae
3.5.4.12
-70C, 20% v/v ethyleneglycol, stable for 2 months
Cricetinae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-hydroxymethyl-dCMP + H2O
-
289008
Cricetinae
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
-
289008
Cricetinae
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289008
Cricetinae
dUMP + NH3
-
289008
Cricetinae
?
3.5.4.12
dCMP + H2O
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
289008
Cricetinae
dUMP + NH3
-
289008
Cricetinae
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.12
37
-
assay at
Cricetinae
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.4.12
additional information
-
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v
Cricetinae
3.5.4.12
50
-
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and Mg2+, or by increasing protein concentration
Cricetinae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8.4
-
BHK-21/C13 cells
Cricetinae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
7
9
pH 7.0: 60%, pH 8.2: 98% and pH 9.0 96% of maximum activity
Cricetinae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
3.5.4.12
additional information
no coenzyme required
Cricetinae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
maximum activation at 0.04 mM, dCTP activation requires presence of Mg2+ or Mn2+; positive allosteric effector
Cricetinae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
3.5.4.12
additional information
no coenzyme required
Cricetinae
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
dCTP and Mg2+ stabilize
Cricetinae
3.5.4.12
dCTP, 0.125 mM, or ethyleneglycol, 20% v/v, stabilize
Cricetinae
3.5.4.12
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v
Cricetinae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor; maximum inhibition at 0.015 mM, inhibition requires presence of Mg2+ or Mn2+
Cricetinae
3.5.4.12
EDTA
1 mM: 90% inhibition, restored to maximum by 1-5 mM Mg2+ or Mn2+
Cricetinae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.91
-
dCMP
-
Cricetinae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+; required for activation by dCTP and for inhibition by dTTP; required for activation by dCTP, Mg2+ in the absence of dCTP: no effect; required for complete activation by dCTP
Cricetinae
3.5.4.12
Mn2+
dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+
Cricetinae
3.5.4.12
additional information
bound bivalent metal cations probably present; metal ions not required
Cricetinae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
115000
-
BHK-21/C13 cells, sucrose density gradient centrifugation
Cricetinae
3.5.4.12
130000
-
BHK-21/C13 cells, gel filtration
Cricetinae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Cricetinae
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
dUMP + NH3
-
Cricetinae
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
partial, BHK-21/C13 cells, baby-hamster
Cricetinae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
BHK-21 cell
baby-hamster kidney
Cricetinae
-
3.5.4.12
kidney
BHK-21/C13 cells, baby-hamster kidney cells
Cricetinae
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.4.12
0.37
-
-
Cricetinae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
-70C
Cricetinae
3.5.4.12
-70C, 20% v/v ethyleneglycol, stable for 2 months
Cricetinae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-hydroxymethyl-dCMP + H2O
-
289008
Cricetinae
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
-
289008
Cricetinae
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289008
Cricetinae
dUMP + NH3
-
289008
Cricetinae
?
3.5.4.12
dCMP + H2O
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
289008
Cricetinae
dUMP + NH3
-
289008
Cricetinae
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.12
37
-
assay at
Cricetinae
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.4.12
additional information
-
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v
Cricetinae
3.5.4.12
50
-
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and Mg2+, or by increasing protein concentration
Cricetinae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8.4
-
BHK-21/C13 cells
Cricetinae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
7
9
pH 7.0: 60%, pH 8.2: 98% and pH 9.0 96% of maximum activity
Cricetinae