BRENDA - Enzyme Database

Deoxycytidylate deaminase. Evidence for a new enzyme in cells infected by the virus of Herpes simplex

Rolton, H.A.; Keir, H.M.; Biochem. J. 143, 403-409 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells
Cricetinae
3.5.4.12
dCTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells
Herpes simplex virus
3.5.4.12
Herpes antiserum
activation of host-cell enzyme
Cricetinae
3.5.4.12
additional information
no activation by KCl up to 0.3 M
Cricetinae
3.5.4.12
additional information
no activation by KCl up to 0.3 M
Herpes simplex virus
3.5.4.12
pre-immune serum
activation of host-cell enzyme, twice as effective as Herpes antiserum
Cricetinae
General Stability
EC Number
General Stability
Organism
3.5.4.12
dCTP and Mg2+ stabilize
Cricetinae
3.5.4.12
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
Herpes simplex virus
3.5.4.12
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
Cricetinae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
Cricetinae
3.5.4.12
dTTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
Herpes simplex virus
3.5.4.12
Herpes antiserum
inhibition of Herpes infected-cell enzyme
Herpes simplex virus
3.5.4.12
additional information
no inhibition by KCl up to 0.3 M
Cricetinae
3.5.4.12
additional information
no inhibition by KCl up to 0.3 M
Herpes simplex virus
3.5.4.12
pre-immune serum
inhibition of Herpes infected-cell enzyme
Herpes simplex virus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.32
-
dCMP
in the presence of dCTP and presence of 2 mM Mg2+
Cricetinae
3.5.4.12
0.32
-
dCMP
in the presence of dCTP and presence of 2 mM Mg2+
Herpes simplex virus
3.5.4.12
0.91
-
dCMP
in the presence of dCTP and absence of 2 mM Mg2+
Cricetinae
3.5.4.12
0.91
-
dCMP
in the presence of dCTP and absence of 2 mM Mg2+
Herpes simplex virus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP; required for complete activation by dCTP
Cricetinae
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP; required for complete activation by dCTP
Herpes simplex virus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Herpes simplex virus
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Cricetinae
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Cricetinae
-
BHK-21/C13 cells, baby-hamster, enzyme from non-infected cells and cells infected by Herpes simplex
-
3.5.4.12
Herpes simplex virus
-
host baby-hamster, BHK-21/C13 cells; induced by
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
BHK-21/C13 cells , enzyme from non-infected cells and cells infected by Herpes simplex
Cricetinae
3.5.4.12
host baby-hamster, BHK-21/C13 cells
Herpes simplex virus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
BHK-21 cell
baby-hamster kidney
Cricetinae
-
3.5.4.12
kidney
BHK-21/C13 cells, baby-hamster kidney cells
Cricetinae
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
289007
Herpes simplex virus
dUMP + NH3
-
289007
Herpes simplex virus
?
3.5.4.12
dCMP + H2O
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
289007
Cricetinae
dUMP + NH3
-
289007
Cricetinae
?
3.5.4.12
dCMP + H2O
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
289007
Herpes simplex virus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
289007
Cricetinae
dUMP + NH3
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Cricetinae
3.5.4.12
37
-
assay at
Herpes simplex virus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.12
additional information
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme
Cricetinae
3.5.4.12
additional information
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme
Herpes simplex virus
3.5.4.12
37
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C
Cricetinae
3.5.4.12
37
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C
Herpes simplex virus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
additional information
-
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum
Cricetinae
3.5.4.12
additional information
-
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum
Herpes simplex virus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells
Cricetinae
3.5.4.12
dCTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells
Herpes simplex virus
3.5.4.12
Herpes antiserum
activation of host-cell enzyme
Cricetinae
3.5.4.12
additional information
no activation by KCl up to 0.3 M
Cricetinae
3.5.4.12
additional information
no activation by KCl up to 0.3 M
Herpes simplex virus
3.5.4.12
pre-immune serum
activation of host-cell enzyme, twice as effective as Herpes antiserum
Cricetinae
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
dCTP and Mg2+ stabilize
Cricetinae
3.5.4.12
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
Herpes simplex virus
3.5.4.12
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
Cricetinae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
Cricetinae
3.5.4.12
dTTP
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
Herpes simplex virus
3.5.4.12
Herpes antiserum
inhibition of Herpes infected-cell enzyme
Herpes simplex virus
3.5.4.12
additional information
no inhibition by KCl up to 0.3 M
Cricetinae
3.5.4.12
additional information
no inhibition by KCl up to 0.3 M
Herpes simplex virus
3.5.4.12
pre-immune serum
inhibition of Herpes infected-cell enzyme
Herpes simplex virus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.32
-
dCMP
in the presence of dCTP and presence of 2 mM Mg2+
Cricetinae
3.5.4.12
0.32
-
dCMP
in the presence of dCTP and presence of 2 mM Mg2+
Herpes simplex virus
3.5.4.12
0.91
-
dCMP
in the presence of dCTP and absence of 2 mM Mg2+
Cricetinae
3.5.4.12
0.91
-
dCMP
in the presence of dCTP and absence of 2 mM Mg2+
Herpes simplex virus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP; required for complete activation by dCTP
Cricetinae
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP; required for complete activation by dCTP
Herpes simplex virus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Herpes simplex virus
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Cricetinae
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
BHK-21/C13 cells , enzyme from non-infected cells and cells infected by Herpes simplex
Cricetinae
3.5.4.12
host baby-hamster, BHK-21/C13 cells
Herpes simplex virus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
BHK-21 cell
baby-hamster kidney
Cricetinae
-
3.5.4.12
kidney
BHK-21/C13 cells, baby-hamster kidney cells
Cricetinae
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
289007
Herpes simplex virus
dUMP + NH3
-
289007
Herpes simplex virus
?
3.5.4.12
dCMP + H2O
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
289007
Cricetinae
dUMP + NH3
-
289007
Cricetinae
?
3.5.4.12
dCMP + H2O
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
289007
Herpes simplex virus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
289007
Cricetinae
dUMP + NH3
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Cricetinae
3.5.4.12
37
-
assay at
Herpes simplex virus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.12
additional information
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme
Cricetinae
3.5.4.12
additional information
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme
Herpes simplex virus
3.5.4.12
37
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C
Cricetinae
3.5.4.12
37
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C
Herpes simplex virus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
additional information
-
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum
Cricetinae
3.5.4.12
additional information
-
enzyme from host-cell and Herpes virus-induced enzyme have a similar pH-optimum
Herpes simplex virus