BRENDA - Enzyme Database

Stabilizing effect of ethyleneglycol on deoxycytidylate deaminase in relation to thermal and ultraviolet inactivation

Dosseva.I.M.; Tomov, T.H.; Dokl. Bolg. Akad. Nauk 28, 241-244 (1975)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Mus musculus
3.5.4.12
dCTP or glycerol stabilize
Mus musculus
3.5.4.12
ethyleneglycol, 20%, stabilizes against thermal and UV inactivation and denaturation factors with different mechanism of action
Mus musculus
3.5.4.12
glycerol stabilizes
Mus musculus
3.5.4.12
some free nucleotides, most effective dCTP, stabilize
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Mus musculus
one of the regulatory enzymes of DNA biosynthesis
dUMP + NH3
-
Mus musculus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Mus musculus
-
Ehrlich's ascites tumor cells and various gastrointestinal tumors
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
partial, Ehrlich's ascites tumor cells
Mus musculus
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289006
Mus musculus
dUMP + NH3
-
289006
Mus musculus
?
3.5.4.12
dCMP + H2O
one of the regulatory enzymes of DNA biosynthesis
289006
Mus musculus
dUMP + NH3
-
289006
Mus musculus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.12
additional information
-
ethyleneglycol stabilizes against thermal and UV inactivation
Mus musculus
3.5.4.12
43
-
in the presence of ethyleneglycol: 100% stable
Mus musculus
3.5.4.12
50
-
ethyleneglycol stabilizes against thermal inactivation, when temperature increases to 50°C, leads to certain increase in specific activity
Mus musculus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Mus musculus
3.5.4.12
dCTP or glycerol stabilize
Mus musculus
3.5.4.12
ethyleneglycol, 20%, stabilizes against thermal and UV inactivation and denaturation factors with different mechanism of action
Mus musculus
3.5.4.12
glycerol stabilizes
Mus musculus
3.5.4.12
some free nucleotides, most effective dCTP, stabilize
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Mus musculus
one of the regulatory enzymes of DNA biosynthesis
dUMP + NH3
-
Mus musculus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
partial, Ehrlich's ascites tumor cells
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289006
Mus musculus
dUMP + NH3
-
289006
Mus musculus
?
3.5.4.12
dCMP + H2O
one of the regulatory enzymes of DNA biosynthesis
289006
Mus musculus
dUMP + NH3
-
289006
Mus musculus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.12
additional information
-
ethyleneglycol stabilizes against thermal and UV inactivation
Mus musculus
3.5.4.12
43
-
in the presence of ethyleneglycol: 100% stable
Mus musculus
3.5.4.12
50
-
ethyleneglycol stabilizes against thermal inactivation, when temperature increases to 50°C, leads to certain increase in specific activity
Mus musculus