BRENDA - Enzyme Database

Deoxycytidylate deaminase from Bacillus subtilis. Purification, characterization, and physiological function

Mollgaard, H.; Neuhard, J.; J. Biol. Chem. 253, 3536-3542 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
2-mercaptoethanol
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
3.5.4.12
CTP
no activation
Bacillus subtilis
3.5.4.12
dCDP
100% activation
Bacillus subtilis
3.5.4.12
dCTP
dCTP not required and not activared by dCTP
Bacillusphage phiE
3.5.4.12
dCTP
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
3.5.4.12
dCTP
dCTP not required and not activared by dCTP
bacteriophage SP8
3.5.4.12
dTTP
no activation
Bacillus subtilis
3.5.4.12
dUTP
89% activation
Bacillus subtilis
3.5.4.12
additional information
no activation by ATP, UTP, CTP and dTTP
Bacillus subtilis
General Stability
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Bacillus subtilis
3.5.4.12
thiols stabilize
Bacillus subtilis
3.5.4.12
thiols, such as 2-mercaptoethanol or dithiothreitol, stabilize, in the absence of thiols the enzyme is highly unstable
Bacillus subtilis
3.5.4.12
Zn2+ or dCTP stabilize, protect against inactivation
Bacillus subtilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
no inhibition by dTTP
bacteriophage SP8
3.5.4.12
EDTA
completely
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
-
Bacillus subtilis
3.5.4.12
0.14
-
dCMP
-
Bacillus subtilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
no activation
Bacillus subtilis
3.5.4.12
Co2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
3.5.4.12
metal ion
required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
3.5.4.12
Mg2+
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+; no activation
Bacillus subtilis
3.5.4.12
Mn2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
3.5.4.12
additional information
metal ions not required
Bacillusphage phiE
3.5.4.12
additional information
metal ion required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
3.5.4.12
additional information
metal ions not required
bacteriophage SP8
3.5.4.12
Ni2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
3.5.4.12
Zn2+
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+
Bacillus subtilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
170000
-
gel filtration and sucrose density gradient centrifugation
Bacillus subtilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Bacillus subtilis
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis
?
3.5.4.12
dCMP + H2O
Bacillus subtilis ED40
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis ED40
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Bacillus subtilis
-
-
-
3.5.4.12
Bacillus subtilis ED40
-
-
-
3.5.4.12
Bacillusphage phiE
-
host Bacillus subtilis
-
3.5.4.12
bacteriophage SP8
-
host Bacillus subtilis
-
3.5.4.12
no activity in Escherichia coli
-
wild type Escherichia coli contains no dCMP deaminase
-
3.5.4.12
no activity in Salmonella typhimurium
-
do not contain dCMP deaminase
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
partial
Bacillus subtilis
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Bacillus subtilis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
6.36
-
-
Bacillus subtilis
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
-70°C, pH 7.8, 2 mM 2-mercaptoethanol
Bacillus subtilis
3.5.4.12
0°C, 2-mercaptoethanol, stable for more than 6 h
Bacillus subtilis
3.5.4.12
0°C, half-life: 30 min
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis
UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis ED40
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
3.5.4.12
dCMP + H2O
-
289005
Bacillusphage phiE
dUMP + NH3
-
289005
Bacillusphage phiE
?
3.5.4.12
dCMP + H2O
-
289005
bacteriophage SP8
dUMP + NH3
-
289005
bacteriophage SP8
?
3.5.4.12
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
3.5.4.12
dCMP + H2O
-
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
3.5.4.12
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
3.5.4.12
additional information
substrate specificity
289005
Bacillus subtilis
?
-
-
-
-
3.5.4.12
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis
?
-
-
-
-
3.5.4.12
additional information
substrate specificity
289005
Bacillus subtilis ED40
?
-
-
-
-
3.5.4.12
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis ED40
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Bacillus subtilis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.12
-
-
half-life: 30 min, Zn2+, thiols, 2-mercaptoethanol, and dCTP protect against inactivation
Bacillus subtilis
3.5.4.12
50
-
thiol, 2-mercaptoethanol, dCTP and Zn2+ protect the enzyme against heat inactivation at 50°C
Bacillus subtilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.8
-
pH optimum around
Bacillus subtilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
7
8.5
50% maximal activity at pH 7.0 and 8.5
Bacillus subtilis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
2-mercaptoethanol
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
3.5.4.12
CTP
no activation
Bacillus subtilis
3.5.4.12
dCDP
100% activation
Bacillus subtilis
3.5.4.12
dCTP
dCTP not required and not activared by dCTP
Bacillusphage phiE
3.5.4.12
dCTP
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
3.5.4.12
dCTP
dCTP not required and not activared by dCTP
bacteriophage SP8
3.5.4.12
dTTP
no activation
Bacillus subtilis
3.5.4.12
dUTP
89% activation
Bacillus subtilis
3.5.4.12
additional information
no activation by ATP, UTP, CTP and dTTP
Bacillus subtilis
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Bacillus subtilis
3.5.4.12
thiols stabilize
Bacillus subtilis
3.5.4.12
thiols, such as 2-mercaptoethanol or dithiothreitol, stabilize, in the absence of thiols the enzyme is highly unstable
Bacillus subtilis
3.5.4.12
Zn2+ or dCTP stabilize, protect against inactivation
Bacillus subtilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
no inhibition by dTTP
bacteriophage SP8
3.5.4.12
EDTA
completely
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
-
Bacillus subtilis
3.5.4.12
0.14
-
dCMP
-
Bacillus subtilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
no activation
Bacillus subtilis
3.5.4.12
Co2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
3.5.4.12
metal ion
required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
3.5.4.12
Mg2+
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+; no activation
Bacillus subtilis
3.5.4.12
Mn2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
3.5.4.12
additional information
metal ions not required
Bacillusphage phiE
3.5.4.12
additional information
metal ion required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
3.5.4.12
additional information
metal ions not required
bacteriophage SP8
3.5.4.12
Ni2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
3.5.4.12
Zn2+
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+
Bacillus subtilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
170000
-
gel filtration and sucrose density gradient centrifugation
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Bacillus subtilis
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis
?
3.5.4.12
dCMP + H2O
Bacillus subtilis ED40
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis ED40
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
partial
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
6.36
-
-
Bacillus subtilis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
-70°C, pH 7.8, 2 mM 2-mercaptoethanol
Bacillus subtilis
3.5.4.12
0°C, 2-mercaptoethanol, stable for more than 6 h
Bacillus subtilis
3.5.4.12
0°C, half-life: 30 min
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis
UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis ED40
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
3.5.4.12
dCMP + H2O
-
289005
Bacillusphage phiE
dUMP + NH3
-
289005
Bacillusphage phiE
?
3.5.4.12
dCMP + H2O
-
289005
bacteriophage SP8
dUMP + NH3
-
289005
bacteriophage SP8
?
3.5.4.12
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
3.5.4.12
dCMP + H2O
-
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
3.5.4.12
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
3.5.4.12
additional information
substrate specificity
289005
Bacillus subtilis
?
-
-
-
-
3.5.4.12
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis
?
-
-
-
-
3.5.4.12
additional information
substrate specificity
289005
Bacillus subtilis ED40
?
-
-
-
-
3.5.4.12
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis ED40
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Bacillus subtilis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.12
-
-
half-life: 30 min, Zn2+, thiols, 2-mercaptoethanol, and dCTP protect against inactivation
Bacillus subtilis
3.5.4.12
50
-
thiol, 2-mercaptoethanol, dCTP and Zn2+ protect the enzyme against heat inactivation at 50°C
Bacillus subtilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.8
-
pH optimum around
Bacillus subtilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
7
8.5
50% maximal activity at pH 7.0 and 8.5
Bacillus subtilis