BRENDA - Enzyme Database

Deoxycytidylate deaminase from T2-infected Escherichia coli

Maley, G.F.; Methods Enzymol. 51, 412-418 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
-
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Enterobacteria phage T2
General Stability
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
3.5.4.12
dCTP, or to a lesser extent dTTP, stabilize against heat inactivation, protein denaturants and proteolytic enzymes
Enterobacteria phage T2
3.5.4.12
thiols stabilize
Enterobacteria phage T2
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T2
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic properties and parameters
Enterobacteria phage T2
3.5.4.12
0.1
-
5-methyl-dCMP
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
Enterobacteria phage T2
3.5.4.12
0.1
-
dCMP
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
Enterobacteria phage T2
3.5.4.12
10
-
CMP
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
Enterobacteria phage T2
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Enterobacteria phage T2
3.5.4.12
divalent cations
reaction completely dependent on divalent cations
Enterobacteria phage T2
3.5.4.12
Mg2+
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Enterobacteria phage T2
3.5.4.12
Mn2+
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Enterobacteria phage T2
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
124000
-
PAGE and sedimentation equilibrium
Enterobacteria phage T2
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
true substrate probably dCMP-Mg complex
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Enterobacteria phage T2
-
T2-phage infected Escherichia coli
-
3.5.4.12
no activity in Escherichia coli
-
enzyme not detectable in Escherichia coli until after infection with T-even bacteriophage
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
T2-phage infected Escherichia coli
Enterobacteria phage T2
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
regulation, allosteric end-product regulation
Enterobacteria phage T2
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.4.12
430
-
-
Enterobacteria phage T2
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
0-4░C, neutral pH, 0.1 M 2-mercaptoethanol, stable for several months
Enterobacteria phage T2
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-bromo-dCMP + H2O
-
289004
Enterobacteria phage T2
5-bromo-dUMP + NH3
-
-
-
?
3.5.4.12
5-chloro-dCMP + H2O
-
289004
Enterobacteria phage T2
5-chloro-dUMP + NH3
-
-
-
?
3.5.4.12
5-fluoro-dCMP + H2O
-
289004
Enterobacteria phage T2
5-fluoro-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
very poor substrate
289004
Enterobacteria phage T2
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-iodo-dCMP + H2O
very poor substrate
289004
Enterobacteria phage T2
5-iodo-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
-
289004
Enterobacteria phage T2
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
-
289004
Enterobacteria phage T2
UMP + NH3
-
-
-
-
3.5.4.12
dCMP + H2O
true substrate probably dCMP-Mg complex
289004
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
true substrate probably dCMP-Mg complex
289004
Enterobacteria phage T2
dUMP + NH3
-
289004
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP
289004
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
substrate specificity
289004
Enterobacteria phage T2
?
-
-
-
-
3.5.4.12
additional information
catalyzes deamination of halogenated derivatives of dCMP, but appears to be restricted in its ability to deaminate substrates with a large group in the 5-position
289004
Enterobacteria phage T2
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.5.4.12
additional information
-
dCTP, and to a lesser extent dTTP, stabilizes against heat inactivation
Enterobacteria phage T2
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
5.3
-
in the absence of dCTP
Enterobacteria phage T2
3.5.4.12
8
-
assay at
Enterobacteria phage T2
3.5.4.12
8.3
-
in the presence of dCTP or 5-hydroxymethyl-dCTP
Enterobacteria phage T2
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
-
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Enterobacteria phage T2
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
2-mercaptoethanol stabilizes
Enterobacteria phage T2
3.5.4.12
dCTP, or to a lesser extent dTTP, stabilize against heat inactivation, protein denaturants and proteolytic enzymes
Enterobacteria phage T2
3.5.4.12
thiols stabilize
Enterobacteria phage T2
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
allosteric inhibitor
Enterobacteria phage T2
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic properties and parameters
Enterobacteria phage T2
3.5.4.12
0.1
-
5-methyl-dCMP
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
Enterobacteria phage T2
3.5.4.12
0.1
-
dCMP
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
Enterobacteria phage T2
3.5.4.12
10
-
CMP
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
Enterobacteria phage T2
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Enterobacteria phage T2
3.5.4.12
divalent cations
reaction completely dependent on divalent cations
Enterobacteria phage T2
3.5.4.12
Mg2+
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Enterobacteria phage T2
3.5.4.12
Mn2+
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Enterobacteria phage T2
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
124000
-
PAGE and sedimentation equilibrium
Enterobacteria phage T2
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
true substrate probably dCMP-Mg complex
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
T2-phage infected Escherichia coli
Enterobacteria phage T2
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.4.12
430
-
-
Enterobacteria phage T2
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
0-4░C, neutral pH, 0.1 M 2-mercaptoethanol, stable for several months
Enterobacteria phage T2
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-bromo-dCMP + H2O
-
289004
Enterobacteria phage T2
5-bromo-dUMP + NH3
-
-
-
?
3.5.4.12
5-chloro-dCMP + H2O
-
289004
Enterobacteria phage T2
5-chloro-dUMP + NH3
-
-
-
?
3.5.4.12
5-fluoro-dCMP + H2O
-
289004
Enterobacteria phage T2
5-fluoro-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
very poor substrate
289004
Enterobacteria phage T2
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-iodo-dCMP + H2O
very poor substrate
289004
Enterobacteria phage T2
5-iodo-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
-
289004
Enterobacteria phage T2
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
-
289004
Enterobacteria phage T2
UMP + NH3
-
-
-
-
3.5.4.12
dCMP + H2O
true substrate probably dCMP-Mg complex
289004
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
true substrate probably dCMP-Mg complex
289004
Enterobacteria phage T2
dUMP + NH3
-
289004
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP
289004
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
substrate specificity
289004
Enterobacteria phage T2
?
-
-
-
-
3.5.4.12
additional information
catalyzes deamination of halogenated derivatives of dCMP, but appears to be restricted in its ability to deaminate substrates with a large group in the 5-position
289004
Enterobacteria phage T2
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.5.4.12
additional information
-
dCTP, and to a lesser extent dTTP, stabilizes against heat inactivation
Enterobacteria phage T2
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
5.3
-
in the absence of dCTP
Enterobacteria phage T2
3.5.4.12
8
-
assay at
Enterobacteria phage T2
3.5.4.12
8.3
-
in the presence of dCTP or 5-hydroxymethyl-dCTP
Enterobacteria phage T2