BRENDA - Enzyme Database

Reversal of the effect of the allosteric ligands of dCMP-aminohydrolase and stabilization of the enzyme in the T form

Raia, C.A.; Nucci, R.; Vaccaro, C.; Sepe, S.; Rella, R.; J. Mol. Biol. 157, 557-570 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
ammonium sulfate
substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP
Equus asinus
3.5.4.12
dCTP
allosteric activator; allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Equus asinus
3.5.4.12
Glutaraldehyde
substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
3.5.4.12
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition
Equus asinus
General Stability
EC Number
General Stability
Organism
3.5.4.12
dCTP or glycerol stabilize
Equus asinus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
5-Hg-dCMP
irreversible competitive inhibitor, in the absence of mercaptoethanol
Equus asinus
3.5.4.12
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
3.5.4.12
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
dGMP
competitive inhibitor
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
dUMP
competitive inhibitor
Equus asinus
3.5.4.12
Glutaraldehyde
25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
3.5.4.12
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP
Equus asinus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic experiments; kinetic properties and parameters
Equus asinus
3.5.4.12
0.26
-
dCMP-Hg-S-CH2-CH2-OH
-
Equus asinus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
120000
-
-
Equus asinus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
dUMP + NH3
-
Equus asinus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Equus asinus
-
donkey; three conformational isomers of dCMPase, the conformation of the enzyme with no ligands, the activated form, R form: dCTP-Mg complex and the inhibited form, T form: dTTP-Mg complex
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme
Equus asinus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
-
Equus asinus
3.5.4.12
23
-
substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
820
-
substrate: dCMP
Equus asinus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-bromo-dCMP + H2O
-
289003
Equus asinus
5-bromo-dUMP + NH3
-
-
-
?
3.5.4.12
5-fluoro-dCMP + H2O
-
289003
Equus asinus
5-fluoro-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
-
289003
Equus asinus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-iodo-dCMP + H2O
-
289003
Equus asinus
5-iodo-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
-
289003
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
arabinosyl-CMP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
at a very low rate
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
effective substrate in presence of dCTP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
poor substrate
289003
Equus asinus
UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
at a low rare, strongly activated by dCTP
289003
Equus asinus
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
not a substrate of the T form, dTTP-induced form of the enzyme
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
3.5.4.12
dCMP + H2O
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
3.5.4.12
dCMP-Hg-S-CH2-CH2-OH + H2O
better substrate of T form: in the presence of dTTP
289003
Equus asinus
dUMP-Hg-S-CH2-CH2-OH + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Equus asinus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
38
-
assay at
Equus asinus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8
-
assay at, substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
ammonium sulfate
substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP
Equus asinus
3.5.4.12
dCTP
allosteric activator; allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Equus asinus
3.5.4.12
Glutaraldehyde
substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
3.5.4.12
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition
Equus asinus
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
dCTP or glycerol stabilize
Equus asinus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
5-Hg-dCMP
irreversible competitive inhibitor, in the absence of mercaptoethanol
Equus asinus
3.5.4.12
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
3.5.4.12
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
dGMP
competitive inhibitor
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
dUMP
competitive inhibitor
Equus asinus
3.5.4.12
Glutaraldehyde
25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
3.5.4.12
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP
Equus asinus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic experiments; kinetic properties and parameters
Equus asinus
3.5.4.12
0.26
-
dCMP-Hg-S-CH2-CH2-OH
-
Equus asinus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
120000
-
-
Equus asinus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
dUMP + NH3
-
Equus asinus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
-
Equus asinus
3.5.4.12
23
-
substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus
3.5.4.12
820
-
substrate: dCMP
Equus asinus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-bromo-dCMP + H2O
-
289003
Equus asinus
5-bromo-dUMP + NH3
-
-
-
?
3.5.4.12
5-fluoro-dCMP + H2O
-
289003
Equus asinus
5-fluoro-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
3.5.4.12
5-hydroxymethyl-dCMP + H2O
-
289003
Equus asinus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
3.5.4.12
5-iodo-dCMP + H2O
-
289003
Equus asinus
5-iodo-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
-
289003
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
arabinosyl-CMP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
at a very low rate
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
effective substrate in presence of dCTP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
poor substrate
289003
Equus asinus
UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
at a low rare, strongly activated by dCTP
289003
Equus asinus
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
not a substrate of the T form, dTTP-induced form of the enzyme
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
3.5.4.12
dCMP + H2O
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
3.5.4.12
dCMP-Hg-S-CH2-CH2-OH + H2O
better substrate of T form: in the presence of dTTP
289003
Equus asinus
dUMP-Hg-S-CH2-CH2-OH + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Equus asinus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
38
-
assay at
Equus asinus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8
-
assay at, substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus