EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.12 | ammonium sulfate | substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP | Equus asinus | |
3.5.4.12 | dCTP | allosteric activator | Equus asinus | |
3.5.4.12 | dCTP | allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH | Equus asinus | |
3.5.4.12 | dTTP | allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration | Equus asinus | |
3.5.4.12 | Glutaraldehyde | substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation | Equus asinus | |
3.5.4.12 | glycerol | substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition | Equus asinus |
EC Number | General Stability | Organism |
---|---|---|
3.5.4.12 | dCTP or glycerol stabilize | Equus asinus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.12 | 5-Hg-dCMP | irreversible competitive inhibitor, in the absence of mercaptoethanol | Equus asinus | |
3.5.4.12 | dAMP | competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate | Equus asinus | |
3.5.4.12 | dCTP | allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH | Equus asinus | |
3.5.4.12 | dGMP | competitive inhibitor | Equus asinus | |
3.5.4.12 | dTTP | allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH | Equus asinus | |
3.5.4.12 | dUMP | competitive inhibitor | Equus asinus | |
3.5.4.12 | Glutaraldehyde | 25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation | Equus asinus | |
3.5.4.12 | glycerol | substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP | Equus asinus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.12 | additional information | - |
additional information | kinetic properties and parameters | Equus asinus | |
3.5.4.12 | additional information | - |
additional information | kinetic experiments | Equus asinus | |
3.5.4.12 | 0.26 | - |
dCMP-Hg-S-CH2-CH2-OH | - |
Equus asinus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.12 | 120000 | - |
- |
Equus asinus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.12 | dCMP + H2O | Equus asinus | catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis | dUMP + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.12 | Equus asinus | - |
donkey | - |
3.5.4.12 | Equus asinus | - |
three conformational isomers of dCMPase, the conformation of the enzyme with no ligands, the activated form, R form: dCTP-Mg complex and the inhibited form, T form: dTTP-Mg complex | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.12 | - |
Equus asinus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.4.12 | dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Equus asinus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.4.12 | spleen | - |
Equus asinus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.4.12 | additional information | - |
- |
Equus asinus |
3.5.4.12 | 23 | - |
substrate: dCMP-Hg-S-CH2-CH2-OH | Equus asinus |
3.5.4.12 | 820 | - |
substrate: dCMP | Equus asinus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.12 | 5-bromo-dCMP + H2O | - |
Equus asinus | 5-bromo-dUMP + NH3 | - |
? | |
3.5.4.12 | 5-fluoro-dCMP + H2O | - |
Equus asinus | 5-fluoro-dUMP + NH3 | - |
? | |
3.5.4.12 | 5-Hg-dCMP + H2O | deamination activated by dTTP and inhibited by dCTP | Equus asinus | 5-Hg-dUMP + NH3 | - |
? | |
3.5.4.12 | 5-Hg-dCMP + H2O | 5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol | Equus asinus | 5-Hg-dUMP + NH3 | - |
? | |
3.5.4.12 | 5-hydroxymethyl-dCMP + H2O | - |
Equus asinus | 5-hydroxymethyl-dUMP + NH3 | - |
? | |
3.5.4.12 | 5-iodo-dCMP + H2O | - |
Equus asinus | 5-iodo-dUMP + NH3 | - |
? | |
3.5.4.12 | 5-methyl-dCMP + H2O | - |
Equus asinus | 5-methyl-dUMP + NH3 | - |
? | |
3.5.4.12 | ara-CMP + H2O | arabinosyl-CMP | Equus asinus | ara-UMP + NH3 | - |
? | |
3.5.4.12 | ara-CMP + H2O | at a very low rate | Equus asinus | ara-UMP + NH3 | - |
? | |
3.5.4.12 | ara-CMP + H2O | effective substrate in presence of dCTP | Equus asinus | ara-UMP + NH3 | - |
? | |
3.5.4.12 | CMP + H2O | poor substrate | Equus asinus | UMP + NH3 | - |
? | |
3.5.4.12 | CMP + H2O | at a low rare, strongly activated by dCTP | Equus asinus | UMP + NH3 | - |
? | |
3.5.4.12 | dCMP + H2O | not a substrate of the T form, dTTP-induced form of the enzyme | Equus asinus | dUMP + NH3 | - |
? | |
3.5.4.12 | dCMP + H2O | catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis | Equus asinus | dUMP + NH3 | - |
? | |
3.5.4.12 | dCMP-Hg-S-CH2-CH2-OH + H2O | better substrate of T form: in the presence of dTTP | Equus asinus | dUMP-Hg-S-CH2-CH2-OH + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.12 | hexamer | 6 identical subunits | Equus asinus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.12 | 38 | - |
assay at | Equus asinus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.12 | 8 | - |
assay at, substrate: dCMP-Hg-S-CH2-CH2-OH | Equus asinus |