BRENDA - Enzyme Database

Analysis of competition for substrate sites in an allosteric enzyme with co-operative kinetics. Effects of dAMP and dUMP on donkey spleen deoxycytidylate aminohydrolase

Mastrantonio, S.; Nucci, R.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.; Eur. J. Biochem. 137, 421-427 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dAMP
at low substrate, dCMP, concentration: activation at higher concentration of either: inhibition
Equus asinus
3.5.4.12
dCTP
allosteric activator; allosteric activator, increases substrate affinity
Equus asinus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dAMP
at low substrate, dCMP, concentration: activation at higher concentration of either: competitive inhibition; competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor
Equus asinus
3.5.4.12
dUMP
competitive inhibition, kinetics and mechanism of inhibition
Equus asinus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic studies, steady-state kinetics, allosteric kinetics
Equus asinus
3.5.4.12
0.024
-
dCMP
in the presence of dCTP
Equus asinus
3.5.4.12
0.037
-
dCMP
in the presence of dCTP
Equus asinus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
120000
-
-
Equus asinus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Equus asinus
-
donkey
-
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; mechanism; regulation, allosteric end-product regulation
Equus asinus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289002
Equus asinus
dUMP + NH3
-
289002
Equus asinus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Equus asinus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dAMP
at low substrate, dCMP, concentration: activation at higher concentration of either: inhibition
Equus asinus
3.5.4.12
dCTP
allosteric activator; allosteric activator, increases substrate affinity
Equus asinus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dAMP
at low substrate, dCMP, concentration: activation at higher concentration of either: competitive inhibition; competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
3.5.4.12
dTTP
allosteric inhibitor
Equus asinus
3.5.4.12
dUMP
competitive inhibition, kinetics and mechanism of inhibition
Equus asinus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic studies, steady-state kinetics, allosteric kinetics
Equus asinus
3.5.4.12
0.024
-
dCMP
in the presence of dCTP
Equus asinus
3.5.4.12
0.037
-
dCMP
in the presence of dCTP
Equus asinus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
120000
-
-
Equus asinus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
289002
Equus asinus
dUMP + NH3
-
289002
Equus asinus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Equus asinus