BRENDA - Enzyme Database

Isolation of a Saccharomyces cerevisiae mutant strain deficient in deoxycytidylate deaminase activity and partial characterization of the enzyme

McIntosh, E.M.; Haynes, R.H.; J. Bacteriol. 158, 644-649 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
severalfold activation, activation requires presence of Mg2+
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
preliminary kinetic studies and kinetic data
Saccharomyces cerevisiae
3.5.4.12
1
-
dCMP
-
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Saccharomyces cerevisiae
deoxycytidine nucleotides are shunted into dTMP biosynthetic pathway via reaction catalyzed by dCMP deaminase
dUMP + NH3
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
no activity in Escherichia coli
-
-
-
3.5.4.12
no activity in Salmonella typhimurium
-
-
-
3.5.4.12
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
partial, strain ACM-13
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
-70°C
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-bromo-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-bromo-dUMP + NH3
-
-
-
?
3.5.4.12
5-chloro-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-chloro-dUMP + NH3
-
-
-
?
3.5.4.12
5-fluoro-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-fluoro-dUMP + NH3
-
-
-
?
3.5.4.12
5-iodo-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-iodo-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
5-methyl-dCMP: very poor substrate
289000
Saccharomyces cerevisiae
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
highly specific for dCMP
289000
Saccharomyces cerevisiae
dUMP + NH3
-
289000
Saccharomyces cerevisiae
?
3.5.4.12
dCMP + H2O
deoxycytidine nucleotides are shunted into dTMP biosynthetic pathway via reaction catalyzed by dCMP deaminase
289000
Saccharomyces cerevisiae
dUMP + NH3
-
289000
Saccharomyces cerevisiae
?
3.5.4.12
additional information
substrate specificity
289000
Saccharomyces cerevisiae
?
-
-
-
-
3.5.4.12
additional information
halogenated deoxycytidylates: very poor substrates
289000
Saccharomyces cerevisiae
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
9.5
broad pH optimum
Saccharomyces cerevisiae
3.5.4.12
8.2
-
assay at
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
severalfold activation, activation requires presence of Mg2+
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
preliminary kinetic studies and kinetic data
Saccharomyces cerevisiae
3.5.4.12
1
-
dCMP
-
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Saccharomyces cerevisiae
deoxycytidine nucleotides are shunted into dTMP biosynthetic pathway via reaction catalyzed by dCMP deaminase
dUMP + NH3
-
Saccharomyces cerevisiae
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
partial, strain ACM-13
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
-70°C
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-bromo-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-bromo-dUMP + NH3
-
-
-
?
3.5.4.12
5-chloro-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-chloro-dUMP + NH3
-
-
-
?
3.5.4.12
5-fluoro-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-fluoro-dUMP + NH3
-
-
-
?
3.5.4.12
5-iodo-dCMP + H2O
very poor substrate
289000
Saccharomyces cerevisiae
5-iodo-dUMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
5-methyl-dCMP: very poor substrate
289000
Saccharomyces cerevisiae
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
highly specific for dCMP
289000
Saccharomyces cerevisiae
dUMP + NH3
-
289000
Saccharomyces cerevisiae
?
3.5.4.12
dCMP + H2O
deoxycytidine nucleotides are shunted into dTMP biosynthetic pathway via reaction catalyzed by dCMP deaminase
289000
Saccharomyces cerevisiae
dUMP + NH3
-
289000
Saccharomyces cerevisiae
?
3.5.4.12
additional information
substrate specificity
289000
Saccharomyces cerevisiae
?
-
-
-
-
3.5.4.12
additional information
halogenated deoxycytidylates: very poor substrates
289000
Saccharomyces cerevisiae
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
9.5
broad pH optimum
Saccharomyces cerevisiae
3.5.4.12
8.2
-
assay at
Saccharomyces cerevisiae