BRENDA - Enzyme Database

Deoxycytidylate deaminase. Purification and some properties of the enzyme isolated from human spleen

Ellims, P.H.; Kao, A.Y.; Chabner, B.A.; J. Biol. Chem. 256, 6335-6340 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
1-beta-D-arabinofuranosylcytosine 5'-triphosphate
low activation, allosteric activator
Homo sapiens
3.5.4.12
CDP
low activation, allosteric activator
Homo sapiens
3.5.4.12
CTP
low activation, allosteric activator
Homo sapiens
3.5.4.12
dAMP
0.025 mM: low activation
Homo sapiens
3.5.4.12
dCDP
low activation, allosteric activator
Homo sapiens
3.5.4.12
dCTP
activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP; allosteric activator; less than 0.001 mM: very high activation
Homo sapiens
3.5.4.12
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition
Homo sapiens
General Stability
EC Number
General Stability
Organism
3.5.4.12
dCTP and MgCl2 stabilize
Homo sapiens
3.5.4.12
dithiothreitol, 2 mM, stabilizes
Homo sapiens
3.5.4.12
glycerol, 15%, stabilizes
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
ADP
slight inhibition
Homo sapiens
3.5.4.12
AMP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
Co2+
1 mM: 75% inhibition
Homo sapiens
3.5.4.12
Cu2+
1 mM: 98% inhibition
Homo sapiens
3.5.4.12
dGDP
slight inhibition
Homo sapiens
3.5.4.12
dGMP
effective competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
dUMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
dUTP
slight inhibition
Homo sapiens
3.5.4.12
EDTA
no inhibition by EDTA
Homo sapiens
3.5.4.12
GMP
slight inhibition
Homo sapiens
3.5.4.12
additional information
no inhibition by EDTA
Homo sapiens
3.5.4.12
TDP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
TMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP
Homo sapiens
3.5.4.12
UTP
slight inhibition
Homo sapiens
3.5.4.12
Zn2+
1 mM: 94% inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic behavior and data
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
3.5.4.12
Mg2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
3.5.4.12
Mn2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
3.5.4.12
additional information
metal ions not required
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
53000
-
2 * 53000, SDS-PAGE
Homo sapiens
3.5.4.12
109500
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
important role in regulation of intracellular TTP levels
dUMP + NH3
-
-
ir
3.5.4.12
dCMP + H2O
Homo sapiens
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
dUMP + NH3
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Homo sapiens
-
human
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
-
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.4.12
10
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
-50░C, 0.05 mM dCTP, 1 mM MgCl2 or 2 mM dithiothreitol, 18 h, 40% activity lost
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
ara-CMP + H2O
arabinosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
1-beta-D-arabinofuranosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
effective substrate in presence of dCTP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
poor substrate
288999
Homo sapiens
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
high degree of specificity of dCMP
288999
Homo sapiens
dUMP + NH3
-
288999
Homo sapiens
ir
3.5.4.12
dCMP + H2O
important role in regulation of intracellular TTP levels
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
3.5.4.12
dCMP + H2O
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
3.5.4.12
additional information
substrate specificity
288999
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
dimer
2 * 53000, SDS-PAGE
Homo sapiens
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.5.4.12
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
additional information
-
pI: 5.10
Homo sapiens
3.5.4.12
6.5
7.3
broad pH optimum
Homo sapiens
3.5.4.12
7.2
-
assay at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
1-beta-D-arabinofuranosylcytosine 5'-triphosphate
low activation, allosteric activator
Homo sapiens
3.5.4.12
CDP
low activation, allosteric activator
Homo sapiens
3.5.4.12
CTP
low activation, allosteric activator
Homo sapiens
3.5.4.12
dAMP
0.025 mM: low activation
Homo sapiens
3.5.4.12
dCDP
low activation, allosteric activator
Homo sapiens
3.5.4.12
dCTP
activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP; allosteric activator; less than 0.001 mM: very high activation
Homo sapiens
3.5.4.12
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.12
dCTP and MgCl2 stabilize
Homo sapiens
3.5.4.12
dithiothreitol, 2 mM, stabilizes
Homo sapiens
3.5.4.12
glycerol, 15%, stabilizes
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
ADP
slight inhibition
Homo sapiens
3.5.4.12
AMP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
Co2+
1 mM: 75% inhibition
Homo sapiens
3.5.4.12
Cu2+
1 mM: 98% inhibition
Homo sapiens
3.5.4.12
dGDP
slight inhibition
Homo sapiens
3.5.4.12
dGMP
effective competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
dUMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
dUTP
slight inhibition
Homo sapiens
3.5.4.12
EDTA
no inhibition by EDTA
Homo sapiens
3.5.4.12
GMP
slight inhibition
Homo sapiens
3.5.4.12
additional information
no inhibition by EDTA
Homo sapiens
3.5.4.12
TDP
slight inhibition, competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
TMP
poor competitive inhibitor, mechanism of inhibition
Homo sapiens
3.5.4.12
TTP
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP
Homo sapiens
3.5.4.12
UTP
slight inhibition
Homo sapiens
3.5.4.12
Zn2+
1 mM: 94% inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic behavior and data
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
3.5.4.12
Mg2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
3.5.4.12
Mn2+
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Homo sapiens
3.5.4.12
additional information
metal ions not required
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
53000
-
2 * 53000, SDS-PAGE
Homo sapiens
3.5.4.12
109500
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Homo sapiens
important role in regulation of intracellular TTP levels
dUMP + NH3
-
-
ir
3.5.4.12
dCMP + H2O
Homo sapiens
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
dUMP + NH3
-
-
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.4.12
10
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
-50░C, 0.05 mM dCTP, 1 mM MgCl2 or 2 mM dithiothreitol, 18 h, 40% activity lost
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
ara-CMP + H2O
arabinosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
1-beta-D-arabinofuranosyl-CMP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
effective substrate in presence of dCTP
288999
Homo sapiens
ara-UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
poor substrate
288999
Homo sapiens
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
high degree of specificity of dCMP
288999
Homo sapiens
dUMP + NH3
-
288999
Homo sapiens
ir
3.5.4.12
dCMP + H2O
important role in regulation of intracellular TTP levels
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
3.5.4.12
dCMP + H2O
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
288999
Homo sapiens
dUMP + NH3
-
-
-
ir
3.5.4.12
additional information
substrate specificity
288999
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
dimer
2 * 53000, SDS-PAGE
Homo sapiens
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.5.4.12
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
additional information
-
pI: 5.10
Homo sapiens
3.5.4.12
6.5
7.3
broad pH optimum
Homo sapiens
3.5.4.12
7.2
-
assay at
Homo sapiens