BRENDA - Enzyme Database

Freezing of dCMP aminohydrolase in the activated conformation by glutaraldehyde

Nucci, R.; Raia, C.A.; Vaccaro, C.; Sepe, S.; Scarano, E.; Rossi, M.; J. Mol. Biol. 124, 133-145 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate, dAMP activates dCMPase 4fold at low substrate concentration, no activating effect on the modified enzyme, glutaraldehyde-dCMPase
Equus asinus
3.5.4.12
dCTP
activate form: dCTP-enzyme; allosteric activator; dCMPase is activated 15.3fold, glutaraldehyde-dCMPase only 1.8fold; glutaraldehyde fixes dCMPase in the activated conformation induced by dCTP
Equus asinus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
3.5.4.12
dTTP
0.01 mM: 100% inhibition. In the presence of glutaraldehyde enzyme is less sensitive to inhibitory effect of dTTP; allosteric inhibitor; inhibited form: dTTP-enzyme; kinetics of inhibition
Equus asinus
3.5.4.12
Glutaraldehyde
25% glutaraldehyde: rapid inhibition; dCTP protects enzyme to a certain extent. Glutaraldehyde fixes dCMPase in the activated conformation induced by dCTP
Equus asinus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic data, kinetics typical of activated enzyme; kinetic experiments; Km-value of modified enzyme, glutaraldehyde-dCMPase, is lower than that of native enzyme
Equus asinus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP
Equus asinus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
20000
-
6 * 20000, SDS-PAGE
Equus asinus
3.5.4.12
120000
-
PAGE
Equus asinus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Equus asinus
-
donkey; three conformational isomers of dCMPase, the conformation of the enzyme with no ligands, the activated form, R form: dCTP-Mg complex and the inhibited form, T form: dTTP-Mg complex
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Equus asinus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
-
Equus asinus
3.5.4.12
720
-
-
Equus asinus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
-
288998
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
deaminated only in the presence of dCTP, strongly activated by dCTP
288998
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
arabinosyl-CMP
288998
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
deaminated only in the presence of dCTP, strongly activated by dCTP
288998
Equus asinus
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
288998
Equus asinus
dUMP + NH3
-
288998
Equus asinus
?
3.5.4.12
additional information
substrate specificity
288998
Equus asinus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 20000, SDS-PAGE; 6 identical subunits
Equus asinus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Equus asinus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
-
assay at
Equus asinus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate, dAMP activates dCMPase 4fold at low substrate concentration, no activating effect on the modified enzyme, glutaraldehyde-dCMPase
Equus asinus
3.5.4.12
dCTP
activate form: dCTP-enzyme; allosteric activator; dCMPase is activated 15.3fold, glutaraldehyde-dCMPase only 1.8fold; glutaraldehyde fixes dCMPase in the activated conformation induced by dCTP
Equus asinus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
3.5.4.12
dTTP
0.01 mM: 100% inhibition. In the presence of glutaraldehyde enzyme is less sensitive to inhibitory effect of dTTP; allosteric inhibitor; inhibited form: dTTP-enzyme; kinetics of inhibition
Equus asinus
3.5.4.12
Glutaraldehyde
25% glutaraldehyde: rapid inhibition; dCTP protects enzyme to a certain extent. Glutaraldehyde fixes dCMPase in the activated conformation induced by dCTP
Equus asinus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetic data, kinetics typical of activated enzyme; kinetic experiments; Km-value of modified enzyme, glutaraldehyde-dCMPase, is lower than that of native enzyme
Equus asinus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required for activation by dCTP and for inhibition by dTTP
Equus asinus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
20000
-
6 * 20000, SDS-PAGE
Equus asinus
3.5.4.12
120000
-
PAGE
Equus asinus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Equus asinus
-
dUMP + NH3
-
Equus asinus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
-
Equus asinus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.12
additional information
-
-
Equus asinus
3.5.4.12
720
-
-
Equus asinus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
-
288998
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
deaminated only in the presence of dCTP, strongly activated by dCTP
288998
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
ara-CMP + H2O
arabinosyl-CMP
288998
Equus asinus
ara-UMP + NH3
-
-
-
?
3.5.4.12
CMP + H2O
deaminated only in the presence of dCTP, strongly activated by dCTP
288998
Equus asinus
UMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
288998
Equus asinus
dUMP + NH3
-
288998
Equus asinus
?
3.5.4.12
additional information
substrate specificity
288998
Equus asinus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 20000, SDS-PAGE; 6 identical subunits
Equus asinus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Equus asinus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
-
assay at
Equus asinus