BRENDA - Enzyme Database

Probing the infra-structure of thymidylate synthase and deoxycytidylate deaminase

Maley, F.; Belfort, M.; Maley, G.; Adv. Enzyme Regul. 22, 413-430 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Gallus gallus
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.5.4.12
-
Enterobacteria phage T2
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
-
Enterobacteria phage T2
3.5.4.12
additional information
-
additional information
-
Gallus gallus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Gallus gallus
enzyme involved in formation of dTMP
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
enzyme involved in formation of dTMP
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Gallus gallus
enzyme within pyrimidine deoxynucleotide pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
enzyme within pyrimidine deoxynucleotide pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Gallus gallus
enzyme plays an important if not rate controlling step in cell division
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
enzyme plays an important if not rate controlling step in cell division
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Gallus gallus
regulating dTMP for DNA synthesis
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
regulating dTMP for DNA synthesis
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Enterobacteria phage T2
-
T2-phage infected Escherichia coli
-
3.5.4.12
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
T2-phage infected Escherichia coli
Enterobacteria phage T2
Reaction
EC Number
Reaction
Commentary
Organism
3.5.4.12
dCMP + H2O = dUMP + NH3
mechanism; regulation, allosteric end-product regulation
Enterobacteria phage T2
3.5.4.12
dCMP + H2O = dUMP + NH3
regulation, allosteric end-product regulation
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
embryo
-
Gallus gallus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
288997
Gallus gallus
dUMP + NH3
-
288997
Gallus gallus
?
3.5.4.12
dCMP + H2O
-
288997
Enterobacteria phage T2
dUMP + NH3
-
288997
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
enzyme involved in formation of dTMP
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme involved in formation of dTMP
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme within pyrimidine deoxynucleotide pathway
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme within pyrimidine deoxynucleotide pathway
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme plays an important if not rate controlling step in cell division
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme plays an important if not rate controlling step in cell division
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
regulating dTMP for DNA synthesis
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
regulating dTMP for DNA synthesis
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Gallus gallus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
5-hydroxymethyl-dCTP
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Enterobacteria phage T2
3.5.4.12
dCTP
positive allosteric effector
Gallus gallus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.5.4.12
-
Enterobacteria phage T2
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
-
Enterobacteria phage T2
3.5.4.12
additional information
-
additional information
-
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Gallus gallus
enzyme involved in formation of dTMP
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
enzyme involved in formation of dTMP
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Gallus gallus
enzyme within pyrimidine deoxynucleotide pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
enzyme within pyrimidine deoxynucleotide pathway
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Gallus gallus
enzyme plays an important if not rate controlling step in cell division
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
enzyme plays an important if not rate controlling step in cell division
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Gallus gallus
regulating dTMP for DNA synthesis
dUMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Enterobacteria phage T2
regulating dTMP for DNA synthesis
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
T2-phage infected Escherichia coli
Enterobacteria phage T2
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
embryo
-
Gallus gallus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
288997
Gallus gallus
dUMP + NH3
-
288997
Gallus gallus
?
3.5.4.12
dCMP + H2O
-
288997
Enterobacteria phage T2
dUMP + NH3
-
288997
Enterobacteria phage T2
?
3.5.4.12
dCMP + H2O
enzyme involved in formation of dTMP
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme involved in formation of dTMP
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme within pyrimidine deoxynucleotide pathway
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme within pyrimidine deoxynucleotide pathway
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme plays an important if not rate controlling step in cell division
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
enzyme plays an important if not rate controlling step in cell division
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
regulating dTMP for DNA synthesis
288997
Gallus gallus
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
regulating dTMP for DNA synthesis
288997
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 identical subunits
Enterobacteria phage T2
3.5.4.12
hexamer
6 identical subunits
Gallus gallus