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Literature summary extracted from

  • Sakslund, H.; Hammerich, O.
    Effects of pH, temperature and reaction products on the performance of an immobilized creatininase-creatinase-sarcosine oxidase enzyme system for creatinine determination (1992), Anal. Chim. Acta, 268, 331-345.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.2.10 glycine
-
 Flavobacterium sp.

General Stability

EC Number General Stability Organism
3.5.2.10 enzyme immobilized have a long-term stability, unused 6 month Flavobacterium sp.
3.5.3.3 unused enzyme in the immobilized creatininase-creatinase-sarcosine oxidase enzyme system maintains activity for at least 6 months. Activity remains unchanged after heavy daily use of 20-30 days Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.10 formaldehyde
-
 Flavobacterium sp.
3.5.2.10 methanol
-
 Flavobacterium sp.
3.5.3.3 sarcosine
-
 Flavobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.10 creatinine + H2O Flavobacterium sp. creatine metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.10 Flavobacterium sp.
-
 Sigma, Cat. No.C-7399, lot 20 Ho349
-
3.5.3.3 Flavobacterium sp.
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.5.2.10 4°C, 0.1 M phosphate buffer, pH 7.7, enzymes controlled-pore glass immobilized Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.10 creatinine + H2O
-
 Flavobacterium sp. creatine
-
 r
3.5.2.10 creatinine + H2O creatine metabolism Flavobacterium sp. ?
-
 ?
3.5.3.3 creatine + H2O
-
 Flavobacterium sp. sarcosine + urea
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.10 25
-
 immobilized coupled enzyme system Flavobacterium sp.
3.5.3.3 25
-
 immobilized creatininase-creatinase-sarcosine oxidase enzyme system Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.3 30
-
 irreversible loss of some of the activity above Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.10 7.7
-
 immobilized coupled enzyme system Flavobacterium sp.
3.5.2.10 8
-
 immobilized Flavobacterium sp.
3.5.3.3 7.7
-
 immobilized creatininase-creatinase-sarcosine oxidase enzyme system Flavobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.10 7.5 8.5 immobilized Flavobacterium sp.