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Literature summary extracted from

  • Schumann, J.; Boehm, G.; Schumacher, G.; Rudolph, R.; Jaenicke, R.
    Stabilization of creatinase from Pseudomonas putida by random mutagenesis (1993), Protein Sci., 2, 1612-1620.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.3 A109V enhanced thermal stability compared to wild-type enzyme Pseudomonas putida
3.5.3.3 A109V/V355M enhanced thermal stability compared to wild-type enzyme, contributions of single point mutations to the thermal stability are additive Pseudomonas putida
3.5.3.3 A109V/V355M/V182I enhanced thermal stability compared to wild-type enzyme, contributions of single point mutations to the thermal stability are additive Pseudomonas putida
3.5.3.3 V182I no thermal stabilization Pseudomonas putida
3.5.3.3 V355M enhanced thermal stability compared to wild-type enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.3 12.5
-
 Creatine mutant A109V Pseudomonas putida
3.5.3.3 13.3
-
 Creatine wild type enzyme Pseudomonas putida
3.5.3.3 13.9
-
 Creatine mutant V355M Pseudomonas putida
3.5.3.3 14.7
-
 Creatine mutant V182I Pseudomonas putida
3.5.3.3 15.6
-
 Creatine mutant A109V/V355M/V182I Pseudomonas putida
3.5.3.3 16.1
-
 Creatine mutant A109V/V355M Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.3 43000
-
 2 * 43000, SDS-PAGE Pseudomonas putida
3.5.3.3 92000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.3 Pseudomonas putida
-
 wild-type enzyme and mutant enzymes A109V, V355M, V181I, A109V/V355M, A109V/V355M/V182I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.3
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.3 creatine + H2O
-
 Pseudomonas putida sarcosine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.3 dimer 2 * 43000, SDS-PAGE Pseudomonas putida