EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.52 | DTT | mouse organ-derived PNGase requires at least 1 mM DTT for maximal activity | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.52 | Cu2+ | - |
Mus musculus | |
3.5.1.52 | Fe3+ | - |
Mus musculus | |
3.5.1.52 | Glycoasparagine | - |
Mus musculus | |
3.5.1.52 | Monoiodoacetic acid | 2 mM, significant inhibition by thiol-modification | Mus musculus | |
3.5.1.52 | additional information | no effect: free peptides formed during reaction, mannose, alpha-methyl-D-mannoside, 5 mM Na2SO4 | Mus musculus | |
3.5.1.52 | N-ethylmaleimide | 2 mM, significant inhibition by thiol-modification, alkylation | Mus musculus | |
3.5.1.52 | Zn2+ | - |
Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.52 | cytosol | - |
Mus musculus | 5829 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.52 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.52 | - |
Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.52 | brain | - |
Mus musculus | - |
3.5.1.52 | kidney | - |
Mus musculus | - |
3.5.1.52 | liver | - |
Mus musculus | - |
3.5.1.52 | spleen | - |
Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.52 | bovine fetuin glycopeptide + H2O | Leu-Asn(Man3Gal3GlcNAc5)-Asp-Ser-Arg | Mus musculus | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.52 | 6.6 | - |
liver PNGase | Mus musculus |
3.5.1.52 | 6.8 | - |
spleen PNGase | Mus musculus |
3.5.1.52 | 7 | - |
L-929 PNGase, brain PNGase, kidney PNGase | Mus musculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.52 | 6.5 | 7 | no activity below 5.5 | Mus musculus |