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Literature summary extracted from

  • Kitajima, K.; Suzuki, T.; Kouchi, Z.; Inoue, S.; Inoue, Y.
    Identification and distribution of peptide:N-glycanase (PNGase) in mouse organs (1995), Arch. Biochem. Biophys., 319, 393-401.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.52 DTT mouse organ-derived PNGase requires at least 1 mM DTT for maximal activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.52 Cu2+
-
Mus musculus
3.5.1.52 Fe3+
-
Mus musculus
3.5.1.52 Glycoasparagine
-
Mus musculus
3.5.1.52 Monoiodoacetic acid 2 mM, significant inhibition by thiol-modification Mus musculus
3.5.1.52 additional information no effect: free peptides formed during reaction, mannose, alpha-methyl-D-mannoside, 5 mM Na2SO4 Mus musculus
3.5.1.52 N-ethylmaleimide 2 mM, significant inhibition by thiol-modification, alkylation Mus musculus
3.5.1.52 Zn2+
-
Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.52 cytosol
-
Mus musculus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.52 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.52
-
Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.52 brain
-
Mus musculus
-
3.5.1.52 kidney
-
Mus musculus
-
3.5.1.52 liver
-
Mus musculus
-
3.5.1.52 spleen
-
Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.52 bovine fetuin glycopeptide + H2O Leu-Asn(Man3Gal3GlcNAc5)-Asp-Ser-Arg Mus musculus ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.52 6.6
-
liver PNGase Mus musculus
3.5.1.52 6.8
-
spleen PNGase Mus musculus
3.5.1.52 7
-
L-929 PNGase, brain PNGase, kidney PNGase Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.52 6.5 7 no activity below 5.5 Mus musculus