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Literature summary extracted from

  • Cooper, A.J.L.; Duffy, T.E.; Meister, A.
    alpha-Keto acid omega-amidase from rat liver (1985), Methods Enzymol., 113, 350-358.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.3 analysis use of soluble omega-amidase-glutamate dehydrogenase to determine alpha-ketoglutaramate in biological samples Homo sapiens
3.5.1.3 analysis use of soluble omega-amidase-glutamate dehydrogenase to determine alpha-ketoglutaramate in biological samples Rattus norvegicus
3.5.1.3 medicine in patients with liver disease and encephalopathy there is a good correlation between degree of neurological dysfunction and increase in alpha-ketoglutaramate in cerebrospinal fluid, omega-amidase is suitable for determination of alpha-ketoglutaramate Homo sapiens

General Stability

EC Number General Stability Organism
3.5.1.3 20-30% glycerol or 2-mercaptoethanol stabilize Rattus norvegicus
3.5.1.3 enzyme quite stable Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.3 5,5'-dithiobis-(2-nitrobenzoic acid) Ellman's reagent Rattus norvegicus
3.5.1.3 glycylglycine competitive inhibitor, Ki: 5 mM Rattus norvegicus
3.5.1.3 iodoacetamide
-
Rattus norvegicus
3.5.1.3 iodoacetate
-
Rattus norvegicus
3.5.1.3 N-ethylmaleimide
-
Rattus norvegicus
3.5.1.3 NH4+ Ki: 10 mM Rattus norvegicus
3.5.1.3 p-mercuribenzoate
-
Rattus norvegicus
3.5.1.3 sulfhydryl reagents
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.3 0.01
-
2-Oxoglutaramate true Km of omega-amidase for alpha-ketoglutaramate open-chain structure may be of the order of 0.01 mM Rattus norvegicus
3.5.1.3 3
-
2-Oxoglutaramate pH 8.5, only 0.3% of alpha-ketoglutaramate exists in open-chain structure Rattus norvegicus
3.5.1.3 3.8
-
2-oxosuccinamate
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.3 cytoplasm
-
Homo sapiens 5737
-
3.5.1.3 cytoplasm
-
Rattus norvegicus 5737
-
3.5.1.3 mitochondrion
-
Rattus norvegicus 5739
-
3.5.1.3 additional information
-
Homo sapiens
-
-
3.5.1.3 additional information liver, contains isozymic forms, mitochondrial and cytosolic, of omega-amidase Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.3 additional information no effect of Ca2+, no activation with 50 mM CaCl2, toward substrates alpha-ketoglutaramate or alpha-ketosuccinamate Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.3 28000
-
liver, cytoplasmic enzyme, 2 * 28000 Rattus norvegicus
3.5.1.3 58000
-
liver, cytoplasmic enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O Mus musculus alpha-ketoglutaramate 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O Homo sapiens alpha-ketoglutaramate 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O Rattus norvegicus alpha-ketoglutaramate 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O Saccharomyces cerevisiae alpha-ketoglutaramate 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O Embryophyta alpha-ketoglutaramate 2-oxoglutarate + NH3
-
?
3.5.1.3 additional information Mus musculus
-
?
-
?
3.5.1.3 additional information Homo sapiens enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine ?
-
?
3.5.1.3 additional information Rattus norvegicus enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.3 Embryophyta
-
-
-
3.5.1.3 Homo sapiens
-
human
-
3.5.1.3 Mus musculus
-
-
-
3.5.1.3 Rattus norvegicus
-
Wistar rat
-
3.5.1.3 Rattus norvegicus
-
liver, contains isozymic forms, mitochondrial and cytosolic, of omega-amidase
-
3.5.1.3 Saccharomyces cerevisiae
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.1.3 soluble enzyme, sensitive to oxidation, full activity easily regenerated by addition of 5 mM 2-mercaptoethanol Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.3 liver, cytosolic and mitochondrial enzyme Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.3 a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 catalytic sequence involves two steps and an acyl-enzyme intermediate Rattus norvegicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.3 enzyme sensitive to oxidation, full activity easily regenerated in a few min by addition of 5 mM 2-mercaptoethanol Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.3 brain
-
Mus musculus
-
3.5.1.3 brain
-
Homo sapiens
-
3.5.1.3 brain
-
Rattus norvegicus
-
3.5.1.3 cerebrospinal fluid
-
Homo sapiens
-
3.5.1.3 kidney
-
Homo sapiens
-
3.5.1.3 kidney
-
Rattus norvegicus
-
3.5.1.3 liver
-
Mus musculus
-
3.5.1.3 liver
-
Rattus norvegicus
-
3.5.1.3 additional information
-
Mus musculus
-
3.5.1.3 additional information
-
Homo sapiens
-
3.5.1.3 additional information omega-amidase is widespread, detected in various rat organs Rattus norvegicus
-
3.5.1.3 Novikoff hepatoma cell
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.3 17.67
-
liver, mitochondrial enzyme Rattus norvegicus
3.5.1.3 42.4
-
liver, soluble, cytoplasmic, enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.5.1.3 cytoplasmic and mitochondrial enzyme, 20% glycerol, 5 mM 2-mercaptoethanol, pH 7.2 Rattus norvegicus
3.5.1.3 enzyme with high specific activity, 30% glycerol, 0.8 mg protein/ml, 11 years, still active Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O alpha-ketoglutaramate Mus musculus 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O alpha-ketoglutaramate Homo sapiens 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O alpha-ketoglutaramate Rattus norvegicus 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O alpha-ketoglutaramate Saccharomyces cerevisiae 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxoglutaramate + H2O alpha-ketoglutaramate Embryophyta 2-oxoglutarate + NH3
-
?
3.5.1.3 2-oxosuccinamate + H2O alpha-ketosuccinamate Rattus norvegicus 2-oxosuccinate + NH3
-
?
3.5.1.3 delta-ethyl 2-oxoglutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 delta-methyl 2-oxoglutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 gamma-ethyl 2-oxoglutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 gamma-methyl 2-oxoglutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 glutaramate + H2O
-
Rattus norvegicus glutarate + NH3
-
?
3.5.1.3 glutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 additional information
-
Mus musculus ?
-
?
3.5.1.3 additional information substrate specificity Rattus norvegicus ?
-
?
3.5.1.3 additional information hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate Rattus norvegicus ?
-
?
3.5.1.3 additional information catalytic sequence involves two steps and an acyl enzyme intermediate Rattus norvegicus ?
-
?
3.5.1.3 additional information catalyzes hydroxaminolysis and transamidation reactions with esters and amides Rattus norvegicus ?
-
?
3.5.1.3 additional information enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine Homo sapiens ?
-
?
3.5.1.3 additional information enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine Rattus norvegicus ?
-
?
3.5.1.3 p-chlorophenyl glutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 p-methylphenyl glutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 phenyl glutarate + H2O
-
Rattus norvegicus ?
-
?
3.5.1.3 succinamate + H2O
-
Rattus norvegicus succinic acid + NH3
-
?
3.5.1.3 succinate + H2O
-
Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.3 dimer liver, cytoplasmic enzyme, 2 * 28000 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.3 additional information
-
mitochondrial enzyme is more heat labile than the soluble enzyme Rattus norvegicus
3.5.1.3 50
-
purified mitochondrial enzyme loses activity with t1/2 of 10 min, purified soluble enzyme loses activity with t1/2 of 3 h Rattus norvegicus
3.5.1.3 60
-
cytoplasmic and mitochondrial enzyme, 10 min: rapid loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.3 8.5
-
assay at Homo sapiens
3.5.1.3 8.5
-
assay at Rattus norvegicus