EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.3 | analysis | use of soluble omega-amidase-glutamate dehydrogenase to determine alpha-ketoglutaramate in biological samples | Homo sapiens |
3.5.1.3 | analysis | use of soluble omega-amidase-glutamate dehydrogenase to determine alpha-ketoglutaramate in biological samples | Rattus norvegicus |
3.5.1.3 | medicine | in patients with liver disease and encephalopathy there is a good correlation between degree of neurological dysfunction and increase in alpha-ketoglutaramate in cerebrospinal fluid, omega-amidase is suitable for determination of alpha-ketoglutaramate | Homo sapiens |
EC Number | General Stability | Organism |
---|---|---|
3.5.1.3 | 20-30% glycerol or 2-mercaptoethanol stabilize | Rattus norvegicus |
3.5.1.3 | enzyme quite stable | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.3 | 5,5'-dithiobis-(2-nitrobenzoic acid) | Ellman's reagent | Rattus norvegicus | |
3.5.1.3 | glycylglycine | competitive inhibitor, Ki: 5 mM | Rattus norvegicus | |
3.5.1.3 | iodoacetamide | - |
Rattus norvegicus | |
3.5.1.3 | iodoacetate | - |
Rattus norvegicus | |
3.5.1.3 | N-ethylmaleimide | - |
Rattus norvegicus | |
3.5.1.3 | NH4+ | Ki: 10 mM | Rattus norvegicus | |
3.5.1.3 | p-mercuribenzoate | - |
Rattus norvegicus | |
3.5.1.3 | sulfhydryl reagents | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.3 | 0.01 | - |
2-Oxoglutaramate | true Km of omega-amidase for alpha-ketoglutaramate open-chain structure may be of the order of 0.01 mM | Rattus norvegicus | |
3.5.1.3 | 3 | - |
2-Oxoglutaramate | pH 8.5, only 0.3% of alpha-ketoglutaramate exists in open-chain structure | Rattus norvegicus | |
3.5.1.3 | 3.8 | - |
2-oxosuccinamate | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.3 | cytoplasm | - |
Homo sapiens | 5737 | - |
3.5.1.3 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
3.5.1.3 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
3.5.1.3 | additional information | - |
Homo sapiens | - |
- |
3.5.1.3 | additional information | liver, contains isozymic forms, mitochondrial and cytosolic, of omega-amidase | Rattus norvegicus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.3 | additional information | no effect of Ca2+, no activation with 50 mM CaCl2, toward substrates alpha-ketoglutaramate or alpha-ketosuccinamate | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 28000 | - |
liver, cytoplasmic enzyme, 2 * 28000 | Rattus norvegicus |
3.5.1.3 | 58000 | - |
liver, cytoplasmic enzyme | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.3 | 2-oxoglutaramate + H2O | Mus musculus | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | Homo sapiens | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | Rattus norvegicus | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | Saccharomyces cerevisiae | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | Embryophyta | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | additional information | Mus musculus | - |
? | - |
? | |
3.5.1.3 | additional information | Homo sapiens | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | ? | - |
? | |
3.5.1.3 | additional information | Rattus norvegicus | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.3 | Embryophyta | - |
- |
- |
3.5.1.3 | Homo sapiens | - |
human | - |
3.5.1.3 | Mus musculus | - |
- |
- |
3.5.1.3 | Rattus norvegicus | - |
Wistar rat | - |
3.5.1.3 | Rattus norvegicus | - |
liver, contains isozymic forms, mitochondrial and cytosolic, of omega-amidase | - |
3.5.1.3 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
3.5.1.3 | soluble enzyme, sensitive to oxidation, full activity easily regenerated by addition of 5 mM 2-mercaptoethanol | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.3 | liver, cytosolic and mitochondrial enzyme | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.1.3 | a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | catalytic sequence involves two steps and an acyl-enzyme intermediate | Rattus norvegicus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.5.1.3 | enzyme sensitive to oxidation, full activity easily regenerated in a few min by addition of 5 mM 2-mercaptoethanol | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.3 | brain | - |
Mus musculus | - |
3.5.1.3 | brain | - |
Homo sapiens | - |
3.5.1.3 | brain | - |
Rattus norvegicus | - |
3.5.1.3 | cerebrospinal fluid | - |
Homo sapiens | - |
3.5.1.3 | kidney | - |
Homo sapiens | - |
3.5.1.3 | kidney | - |
Rattus norvegicus | - |
3.5.1.3 | liver | - |
Mus musculus | - |
3.5.1.3 | liver | - |
Rattus norvegicus | - |
3.5.1.3 | additional information | - |
Mus musculus | - |
3.5.1.3 | additional information | - |
Homo sapiens | - |
3.5.1.3 | additional information | omega-amidase is widespread, detected in various rat organs | Rattus norvegicus | - |
3.5.1.3 | Novikoff hepatoma cell | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 17.67 | - |
liver, mitochondrial enzyme | Rattus norvegicus |
3.5.1.3 | 42.4 | - |
liver, soluble, cytoplasmic, enzyme | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.3 | cytoplasmic and mitochondrial enzyme, 20% glycerol, 5 mM 2-mercaptoethanol, pH 7.2 | Rattus norvegicus |
3.5.1.3 | enzyme with high specific activity, 30% glycerol, 0.8 mg protein/ml, 11 years, still active | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.3 | 2-oxoglutaramate + H2O | alpha-ketoglutaramate | Mus musculus | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | alpha-ketoglutaramate | Homo sapiens | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | alpha-ketoglutaramate | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | alpha-ketoglutaramate | Saccharomyces cerevisiae | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxoglutaramate + H2O | alpha-ketoglutaramate | Embryophyta | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxosuccinamate + H2O | alpha-ketosuccinamate | Rattus norvegicus | 2-oxosuccinate + NH3 | - |
? | |
3.5.1.3 | delta-ethyl 2-oxoglutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | delta-methyl 2-oxoglutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | gamma-ethyl 2-oxoglutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | gamma-methyl 2-oxoglutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | glutaramate + H2O | - |
Rattus norvegicus | glutarate + NH3 | - |
? | |
3.5.1.3 | glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | additional information | - |
Mus musculus | ? | - |
? | |
3.5.1.3 | additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
3.5.1.3 | additional information | hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate | Rattus norvegicus | ? | - |
? | |
3.5.1.3 | additional information | catalytic sequence involves two steps and an acyl enzyme intermediate | Rattus norvegicus | ? | - |
? | |
3.5.1.3 | additional information | catalyzes hydroxaminolysis and transamidation reactions with esters and amides | Rattus norvegicus | ? | - |
? | |
3.5.1.3 | additional information | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | Homo sapiens | ? | - |
? | |
3.5.1.3 | additional information | enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine | Rattus norvegicus | ? | - |
? | |
3.5.1.3 | p-chlorophenyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | p-methylphenyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | phenyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.5.1.3 | succinamate + H2O | - |
Rattus norvegicus | succinic acid + NH3 | - |
? | |
3.5.1.3 | succinate + H2O | - |
Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.3 | dimer | liver, cytoplasmic enzyme, 2 * 28000 | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | additional information | - |
mitochondrial enzyme is more heat labile than the soluble enzyme | Rattus norvegicus |
3.5.1.3 | 50 | - |
purified mitochondrial enzyme loses activity with t1/2 of 10 min, purified soluble enzyme loses activity with t1/2 of 3 h | Rattus norvegicus |
3.5.1.3 | 60 | - |
cytoplasmic and mitochondrial enzyme, 10 min: rapid loss of activity | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 8.5 | - |
assay at | Homo sapiens |
3.5.1.3 | 8.5 | - |
assay at | Rattus norvegicus |