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Literature summary extracted from

  • Gentzen, I.; Löffler, H.G.; Schneider, F.
    Aminoacylase from Aspergillus oryzae. Comparison with the pig kidney enzyme (1980), Z. Naturforsch. C, 35, 544-550.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 1,10-phenanthroline
-
 Sus scrofa
3.5.1.14 Cd2+
-
 Aspergillus oryzae
3.5.1.14 Cu2+
-
 Aspergillus oryzae
3.5.1.14 diethyldicarbonate
-
 Aspergillus oryzae
3.5.1.14 diethyldicarbonate
-
 Sus scrofa
3.5.1.14 Mn2+
-
 Aspergillus oryzae
3.5.1.14 N-alpha-p-Tosyl-L-lysine
-
 Sus scrofa
3.5.1.14 Ni2+
-
 Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Sus scrofa
3.5.1.14 Co2+ enzyme strongly activated by Co2+ ions Aspergillus oryzae
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Sus scrofa
3.5.1.14 Zn2+ enzyme contains Zn as prosthetic metal Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 36600
-
 2 * 36600, SDS-PAGE Aspergillus oryzae
3.5.1.14 73200
-
-
 Aspergillus oryzae
3.5.1.14 85500
-
-
 Sus scrofa
3.5.1.14 135000
-
 gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Aspergillus oryzae
-
-
-
3.5.1.14 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14
-
 Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.5.1.14 4°C, 0.03 M phosphate buffer, 0.4 M NaCl, pH 7.3 may be stored for several months with negligible loss of activity Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
3.5.1.14 N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
3.5.1.14 N-chloroacetylglutamine-p-aminobenzoate + H2O
-
 Aspergillus oryzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 dimer 2 * 36600, SDS-PAGE Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.14 60
-
 denaturation at 60°C Sus scrofa
3.5.1.14 60
-
 denaturation at 60°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 8
-
-
 Sus scrofa
3.5.1.14 8.5
-
-
 Sus scrofa
3.5.1.14 8.5
-
 N-chloroacetyl-alanine as substrate, Zn2+ enzyme Aspergillus oryzae