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Literature summary extracted from

  • Ferre, H.; Borberg, A.; Duus, J.OE.; Thomsen, K.K.
    A novel type of arabinoxylan arabinofuranohydrolase isolated from germinated barley. Analysis of substrate preference and specificity by nano-probe NMR (2000), Eur. J. Biochem., 267, 6633-6641.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.5
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 60000
-
 isoenzyme Ara1, SDS-PAGE Hordeum vulgare
3.2.1.55 66000
-
 isoenzyme Ara1, SDS-PAGE Hordeum vulgare
3.2.1.55 69000
-
 isoenzyme Ara3, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus awamori
-
-
-
3.2.1.55 Bifidobacterium adolescentis
-
-
-
3.2.1.55 Hordeum vulgare
-
 barley, cv. Maud
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 3 isoenzymes, Ara1, Ara2 and Ara3 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 seedling malt Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 4.184
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 L-arabinoxylan + H2O
-
 Hordeum vulgare L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Bifidobacterium adolescentis L-arabinose + ?
-
 ir
3.2.1.55 additional information no activity against p-nitrophenyl alpha-L-arabinopyranoside or p-nitrophenyl beta-D-xylopyranoside, inactive against linear or branched-chain arabinan Hordeum vulgare ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Hordeum vulgare L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium adolescentis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori L-arabinose + phenol
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 arabinoxylan arabinofuranohydrolase
-
 Hordeum vulgare
3.2.1.55 arabinoxylan arabinofuranohydrolase
-
 Bifidobacterium adolescentis
3.2.1.55 arabinoxylan arabinofuranohydrolase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 60
-
-
 Hordeum vulgare
3.2.1.55 60
-
-
 Aspergillus awamori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 30 80
-
 Hordeum vulgare
3.2.1.55 50 70
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 60
-
 half-life 8 min Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4.2
-
-
 Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 3.5 5
-
 Hordeum vulgare