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Literature summary extracted from

  • De Ioannes, P.; Peirano, A.; Steiner, J.; Eyzaguirre, J.
    An alpha-L-arabinofuranosidase from Penicillium purpurogenum: Production, purification and properties (2000), J. Biotechnol., 76, 253-258.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 arabitol best inducer Talaromyces purpureogenus
3.2.1.55 arabitol best inducer Aspergillus terreus

Application

EC Number Application Comment Organism
3.2.1.55 paper production
-
 Aspergillus niger
3.2.1.55 paper production
-
 Aspergillus terreus
3.2.1.55 paper production enzyme may take part in the cellulose pulp biobleaching process Talaromyces purpureogenus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 ABF2 gene cloned and expressed in Saccharomyces cerevisiae Aspergillus niger
3.2.1.55 abfB gene, nucleotide sequence determined Aspergillus nidulans
3.2.1.55 alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.48
-
 p-nitrophenyl-alpha-L-arabinofuranoside strain B Aspergillus niger
3.2.1.55 1.2
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Trichoderma reesei
3.2.1.55 1.23
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Talaromyces purpureogenus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular
-
 Aspergillus nidulans
-
-
3.2.1.55 extracellular
-
 Aspergillus niger
-
-
3.2.1.55 extracellular
-
 Talaromyces purpureogenus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 51000
-
 gel filtration Talaromyces purpureogenus
3.2.1.55 53000
-
-
 Aspergillus niger
3.2.1.55 53000
-
-
 Trichoderma reesei
3.2.1.55 58000
-
 SDS-PAGE Talaromyces purpureogenus
3.2.1.55 60000
-
-
 Aspergillus niger
3.2.1.55 62000
-
-
 Aspergillus awamori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 beet arabinan + H2O Aspergillus nidulans
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Talaromyces purpureogenus
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Aspergillus terreus
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Aspergillus niger B
-
 L-arabinose + ?
-
 ir
3.2.1.55 birchwood xylan + H2O Aspergillus nidulans
-
 L-arabinose + ?
-
 ?
3.2.1.55 birchwood xylan + H2O Talaromyces purpureogenus
-
 L-arabinose + ?
-
 ?
3.2.1.55 larchwood arabinogalactan + H2O Talaromyces purpureogenus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Talaromyces purpureogenus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus terreus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Trichoderma reesei
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus awamori
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus awamori IFO 4033
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat bran + H2O Talaromyces purpureogenus
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat bran + H2O Aspergillus terreus
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat straw xylan + H2O Talaromyces purpureogenus
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus awamori
-
 IFO4033
-
3.2.1.55 Aspergillus awamori IFO 4033
-
 IFO4033
-
3.2.1.55 Aspergillus nidulans
-
-
-
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Aspergillus niger B
-
-
-
3.2.1.55 Aspergillus terreus
-
-
-
3.2.1.55 Aureobasidium pullulans
-
-
-
3.2.1.55 Penicillium capsulatum
-
-
-
3.2.1.55 Talaromyces purpureogenus
-
-
-
3.2.1.55 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Talaromyces purpureogenus
3.2.1.55
-
 Aspergillus terreus
3.2.1.55
-
 Aspergillus awamori
3.2.1.55
-
 Aureobasidium pullulans
3.2.1.55 2 isoenzymes Penicillium capsulatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 58.4
-
-
 Talaromyces purpureogenus

Storage Stability

EC Number Storage Stability Organism
3.2.1.55 4°C, at pH 2.5-7.5 retains more than 60% activity after 3 days, at pH 9 loses 90% of its activity in 20 h Talaromyces purpureogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 aryl-alpha-arabinofuranoside + H2O
-
 Talaromyces purpureogenus L-arabinose + aryl alcohol
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Aspergillus nidulans L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Talaromyces purpureogenus L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Aspergillus terreus L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Aspergillus niger B L-arabinose + ?
-
 ir
3.2.1.55 birchwood xylan + H2O
-
 Aspergillus nidulans L-arabinose + ?
-
 ?
3.2.1.55 birchwood xylan + H2O
-
 Talaromyces purpureogenus L-arabinose + ?
-
 ?
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Talaromyces purpureogenus L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger B L-arabinose + ?
-
 ir
3.2.1.55 larchwood arabinogalactan + H2O
-
 Talaromyces purpureogenus L-arabinose + ?
-
 ?
3.2.1.55 methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
 Talaromyces purpureogenus methylumbelliferone
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus terreus ?
-
 ?
3.2.1.55 additional information less than 2% activity with p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-alpha-D-galactopyranoside Talaromyces purpureogenus ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger B ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Talaromyces purpureogenus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus terreus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus awamori L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus awamori IFO 4033 L-arabinose + ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Talaromyces purpureogenus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus terreus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger B p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus nidulans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Talaromyces purpureogenus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus terreus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Penicillium capsulatum L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aureobasidium pullulans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori IFO 4033 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger B L-arabinose + phenol
-
 ir
3.2.1.55 wheat bran + H2O
-
 Talaromyces purpureogenus L-arabinose + ?
-
 ?
3.2.1.55 wheat bran + H2O
-
 Aspergillus terreus L-arabinose + ?
-
 ?
3.2.1.55 wheat straw xylan + H2O
-
 Talaromyces purpureogenus L-arabinose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 monomer
-
 Aspergillus niger
3.2.1.55 monomer
-
 Trichoderma reesei
3.2.1.55 monomer 1 * 58000, SDS-PAGE Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 50
-
-
 Aspergillus niger
3.2.1.55 50
-
-
 Talaromyces purpureogenus
3.2.1.55 50
-
-
 Aspergillus terreus
3.2.1.55 60
-
-
 Aspergillus niger
3.2.1.55 60
-
-
 Aspergillus terreus
3.2.1.55 60
-
-
 Trichoderma reesei
3.2.1.55 60
-
-
 Aspergillus awamori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 50 55 half-life of 2 h at 50°C and of 25 min at 55°C Talaromyces purpureogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 3.7
-
-
 Aspergillus niger
3.2.1.55 4
-
-
 Aspergillus niger
3.2.1.55 4
-
-
 Talaromyces purpureogenus
3.2.1.55 4
-
-
 Trichoderma reesei