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Literature summary extracted from

  • Debeche, T.; Cummings, N.; Connerton, I.; Debeire, P.; O'Donohue, M.J.
    Genetic and biochemical characterization of a highly thermostable alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus (2000), Appl. Environ. Microbiol., 66, 1734-1736.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 Triton X-100
-
 Thermobacillus xylanilyticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55
-
 Streptomyces lividans
3.2.1.55
-
 Thermoclostridium stercorarium
3.2.1.55 arfI and arfII, genes encoding alpha-L-arabinofuranosidase Bifidobacterium adolescentis
3.2.1.55 gene AbfD3 isolated, inserted into pET24 vector and expressed in Escherichia coli Thermobacillus xylanilyticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.55
-
 Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Co2+
-
 Thermobacillus xylanilyticus
3.2.1.55 Cu2+
-
 Aspergillus terreus
3.2.1.55 Cu2+
-
 Thermobacillus xylanilyticus
3.2.1.55 Cu2+
-
 Thermoclostridium stercorarium
3.2.1.55 Hg2+
-
 Aspergillus terreus
3.2.1.55 Hg2+ 1 mM HgCl2 inhibits the activity by 98% Geobacillus stearothermophilus
3.2.1.55 Hg2+
-
 Streptomyces purpurascens
3.2.1.55 Hg2+ 1 mM HgCl2 inhibits the activity by 98% Thermobacillus xylanilyticus
3.2.1.55 Hg2+
-
 Thermoclostridium stercorarium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.5
-
 p-nitrophenyl-alpha-L-arabinose 75°C Thermobacillus xylanilyticus
3.2.1.55 0.72
-
 p-nitrophenyl-alpha-L-arabinose 60°C Thermobacillus xylanilyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 56000
-
 SDS-PAGE Thermobacillus xylanilyticus
3.2.1.55 56070
-
 amino acid sequence analysis Thermobacillus xylanilyticus
3.2.1.55 56090
-
 mass spectrometry Thermobacillus xylanilyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 larchwood xylan + H2O Thermobacillus xylanilyticus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Geobacillus stearothermophilus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus terreus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus awamori
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Thermoclostridium stercorarium
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Thermobacillus xylanilyticus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus awamori IFO 4033
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O Aspergillus awamori
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O Thermobacillus xylanilyticus
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O Aspergillus awamori IFO 4033
-
 L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O Thermobacillus xylanilyticus D3
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus awamori
-
 IFO4033
-
3.2.1.55 Aspergillus awamori IFO 4033
-
 IFO4033
-
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Aspergillus terreus
-
-
-
3.2.1.55 Aureobasidium pullulans
-
-
-
3.2.1.55 Bacillus subtilis
-
 strain 3-6
-
3.2.1.55 Bacillus subtilis 03. Jun
-
 strain 3-6
-
3.2.1.55 Bifidobacterium adolescentis
-
-
-
3.2.1.55 Breznakibacter xylanolyticus
-
-
-
3.2.1.55 Geobacillus stearothermophilus
-
-
-
3.2.1.55 Raphanus sativus
-
 radish
-
3.2.1.55 Streptomyces lividans
-
 66strain 1326
-
3.2.1.55 Streptomyces lividans 66 1326
-
 66strain 1326
-
3.2.1.55 Streptomyces purpurascens
-
 IFO 3389
-
3.2.1.55 Thermobacillus xylanilyticus
-
 D3
-
3.2.1.55 Thermobacillus xylanilyticus D3
-
 D3
-
3.2.1.55 Thermoclostridium stercorarium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Geobacillus stearothermophilus
3.2.1.55
-
 Aspergillus niger
3.2.1.55
-
 Bifidobacterium adolescentis
3.2.1.55
-
 Streptomyces lividans
3.2.1.55 recombinant protein, expressed in Escherichia coli Thermobacillus xylanilyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 seed
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 350
-
 60°C Thermobacillus xylanilyticus
3.2.1.55 490
-
 75°C Thermobacillus xylanilyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 L-arabinoxylan + H2O
-
 Geobacillus stearothermophilus L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Bifidobacterium adolescentis L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces lividans L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Thermobacillus xylanilyticus L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Thermobacillus xylanilyticus D3 L-arabinose + ?
-
 ir
3.2.1.55 larchwood xylan + H2O
-
 Thermobacillus xylanilyticus L-arabinose + ?
-
 ?
3.2.1.55 additional information
-
 Bacillus subtilis ?
-
 ?
3.2.1.55 additional information
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus terreus ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces lividans ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces purpurascens ?
-
 ?
3.2.1.55 additional information does not hydrolyze p-nitrophenyl-alpha-L-arabinopyranoside or gum arabic Thermobacillus xylanilyticus ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces lividans 66 1326 ?
-
 ?
3.2.1.55 additional information
-
 Bacillus subtilis 03. Jun ?
-
 ?
3.2.1.55 additional information does not hydrolyze p-nitrophenyl-alpha-L-arabinopyranoside or gum arabic Thermobacillus xylanilyticus D3 ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Geobacillus stearothermophilus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus terreus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus awamori L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Thermobacillus xylanilyticus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus awamori IFO 4033 L-arabinose + ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus terreus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Thermobacillus xylanilyticus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis 03. Jun p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Thermobacillus xylanilyticus D3 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinopyranoside + H2O
-
 Thermobacillus xylanilyticus p-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Raphanus sativus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium adolescentis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus terreus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aureobasidium pullulans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Thermobacillus xylanilyticus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Breznakibacter xylanolyticus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori IFO 4033 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis 03. Jun L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Thermobacillus xylanilyticus D3 L-arabinose + phenol
-
 ir
3.2.1.55 wheat L-arabino-D-xylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O
-
 Aspergillus awamori L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O
-
 Thermobacillus xylanilyticus L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O
-
 Aspergillus awamori IFO 4033 L-arabinose + ?
-
 ?
3.2.1.55 wheat L-arabino-D-xylan + H2O
-
 Thermobacillus xylanilyticus D3 L-arabinose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 arabinoxylan arabinofuranohydrolase
-
 Bifidobacterium adolescentis
3.2.1.55 arabinoxylan arabinofuranohydrolase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 75
-
-
 Thermobacillus xylanilyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 90
-
 highly stable up to Thermobacillus xylanilyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 3.7
-
 strain B Aspergillus niger
3.2.1.55 5.6 6.2
-
 Thermobacillus xylanilyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4 11
-
 Thermobacillus xylanilyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 4 12
-
 Thermobacillus xylanilyticus