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Literature summary extracted from

  • Schwarz, W.H.; Bronnenmeier, K.; Krause, B.; Lottspeich, F.; Staudenbauer, W.L.
    Debranching of arabinoxylan: Properties of the thermoactive recombinant alpha-L-arabinofuranosidase from Clostridium stercoarium (ArfB) (1995), Appl. Microbiol. Biotechnol., 43, 856-860.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.55 analysis
-
Aspergillus niger
3.2.1.55 analysis enzymatic tool for structural analysis of oligo- and polysaccharides Thermoclostridium stercorarium
3.2.1.55 degradation application potential for industrial purposes, biomass degradation and refining, extremely low end-product inhibition by arabinose further increases its applicability Thermoclostridium stercorarium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 gene, encoding AF, heterologously expressed in Escherichia coli JM83 Thermoclostridium stercorarium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Cu2+
-
Thermoclostridium stercorarium
3.2.1.55 ethanol slight inhibition Thermoclostridium stercorarium
3.2.1.55 Hg2+
-
Geobacillus stearothermophilus
3.2.1.55 Hg2+
-
Streptomyces purpurascens
3.2.1.55 Hg2+
-
Thermoclostridium stercorarium
3.2.1.55 p-hydroxymercuribenzoate
-
Thermoclostridium stercorarium
3.2.1.55 SDS
-
Thermoclostridium stercorarium
3.2.1.55 Zn2+
-
Thermoclostridium stercorarium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular
-
Geobacillus stearothermophilus
-
-
3.2.1.55 extracellular
-
Aspergillus niger
-
-
3.2.1.55 extracellular
-
Ruminococcus albus
-
-
3.2.1.55 extracellular
-
Thermoclostridium stercorarium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 52000
-
zymogram staining Thermoclostridium stercorarium
3.2.1.55 75000
-
4 * 75000, SDS-PAGE Ruminococcus albus
3.2.1.55 195000
-
gel filtration Thermoclostridium stercorarium
3.2.1.55 310000
-
-
Ruminococcus albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 oat spelt xylan + H2O Geobacillus stearothermophilus
-
L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O Aspergillus niger
-
L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O Butyrivibrio fibrisolvens
-
L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O Thermoclostridium stercorarium
-
L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O Butyrivibrio fibrisolvens GS113
-
L-arabinose + ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.55 Ethanol resistance to ethanol, neutral detergents and NaCl Thermoclostridium stercorarium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Butyrivibrio fibrisolvens
-
GS113
-
3.2.1.55 Butyrivibrio fibrisolvens GS113
-
GS113
-
3.2.1.55 Geobacillus stearothermophilus
-
-
-
3.2.1.55 Ruminococcus albus
-
8
-
3.2.1.55 Ruminococcus albus 8
-
8
-
3.2.1.55 Streptomyces purpurascens
-
-
-
3.2.1.55 Thermoclostridium stercorarium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
Ruminococcus albus
3.2.1.55 recombinant protein, arfB gene product Thermoclostridium stercorarium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 883
-
recombinant arfB gene product Thermoclostridium stercorarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl beta-D-glucopyranoside + H2O 0.26% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside Thermoclostridium stercorarium 4-nitrophenol + D-glucopyranose
-
?
3.2.1.55 alkyl-alpha-arabinofuranoside + H2O
-
Thermoclostridium stercorarium L-arabinose + alkyl alcohol
-
?
3.2.1.55 aryl-alpha-arabinofuranoside + H2O
-
Thermoclostridium stercorarium L-arabinose + aryl alcohol
-
?
3.2.1.55 L-arabinogalactan + H2O
-
Geobacillus stearothermophilus L-arabinose + ?
-
?
3.2.1.55 L-arabinogalactan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
3.2.1.55 L-arabinogalactan + H2O
-
Thermoclostridium stercorarium L-arabinose + ?
-
?
3.2.1.55 L-arabinoxylan + H2O
-
Geobacillus stearothermophilus L-arabinose + ?
-
ir
3.2.1.55 L-arabinoxylan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
3.2.1.55 L-arabinoxylan + H2O
-
Thermoclostridium stercorarium L-arabinose + ?
-
ir
3.2.1.55 methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens methylumbelliferone
-
?
3.2.1.55 methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
Thermoclostridium stercorarium methylumbelliferone
-
?
3.2.1.55 methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens GS113 methylumbelliferone
-
?
3.2.1.55 oat spelt xylan + H2O
-
Geobacillus stearothermophilus L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O
-
Butyrivibrio fibrisolvens L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O
-
Thermoclostridium stercorarium L-arabinose + ?
-
?
3.2.1.55 oat spelt xylan + H2O
-
Butyrivibrio fibrisolvens GS113 L-arabinose + ?
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus p-nitrophenol + L-arabinose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus niger p-nitrophenol + L-arabinose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Ruminococcus albus p-nitrophenol + L-arabinose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Thermoclostridium stercorarium p-nitrophenol + L-arabinose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens p-nitrophenol + L-arabinose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Ruminococcus albus 8 p-nitrophenol + L-arabinose
-
?
3.2.1.55 p-nitrophenyl-beta-D-galactopyranoside + H2O 0.33% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside Thermoclostridium stercorarium 4-nitrophenol + beta-D-galactopyranose
-
?
3.2.1.55 p-nitrophenyl-beta-D-xylopyranoside + H2O 0.44% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside Thermoclostridium stercorarium 4-nitrophenol + beta-D-xylopyranose
-
?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus L-arabinose + phenol
-
ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Aspergillus niger L-arabinose + phenol
-
ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Ruminococcus albus L-arabinose + phenol
-
ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens L-arabinose + phenol
-
ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Thermoclostridium stercorarium L-arabinose + phenol
-
ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens L-arabinose + phenol
-
ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Ruminococcus albus 8 L-arabinose + phenol
-
ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens GS113 L-arabinose + phenol
-
ir

Subunits

EC Number Subunits Comment Organism
3.2.1.55 tetramer
-
Geobacillus stearothermophilus
3.2.1.55 tetramer 4 * 52000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.55 tetramer 4 * 75000, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 70
-
-
Geobacillus stearothermophilus
3.2.1.55 70
-
-
Thermoclostridium stercorarium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 30 90
-
Thermoclostridium stercorarium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 70 90 remarkably thermostable, half of its activity remains after 0.7 h at 90°C Thermoclostridium stercorarium
3.2.1.55 85
-
recombinant arfB gene product, stable up to Thermoclostridium stercorarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
-
Aspergillus niger
3.2.1.55 5
-
-
Thermoclostridium stercorarium