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Literature summary extracted from
- Mammalian endonuclease, DNase V. Purification and properties of enzyme of calf thymus (1977), J. Biol. Chem., 252, 116-124.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.7 | (NH4)2SO4 | 50% inhibition at 25 mM, inhibition of formation of acid-soluble products | Bos taurus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.21.7 | nucleus | - |
Bos taurus | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.7 | Mg2+ | strong requirement, completely inactive if absent | Bos taurus | |
| 3.1.21.7 | Mn2+ | at 2 mM 50% of the Mg2+-enhanced activity | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.7 | 13000 | - |
4 * 13000, SDS-PAGE | Bos taurus |
| 3.1.21.7 | 53000 | - |
sucrose density gradient centrifugation | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.7 | DNA + H2O | Bos taurus | cleavage of both denatured and native eukaryotic DNA | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.7 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.7 | to homogeneity, chromatography techniques | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.21.7 | thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.7 | 0.52 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.7 | DNA + H2O | cleavage of both denatured and native eukaryotic DNA | Bos taurus | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.7 | tetramer | 4 * 13000, SDS-PAGE | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.7 | 6.6 | - |
- |
Bos taurus |
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Release 2023.1 (January 2023)
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