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Literature summary extracted from
- Purification and partial characterization from the acidophilic sulphur oxidizer Thiobacillus acidophilus (1992), Eur. J. Biochem., 209, 367-374.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.12.1.1 | sulfate | maximum activity at 1.4-2.0 M | Acidiphilium acidophilum |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.12.1.1 | HgCl2 | - |
Acidiphilium acidophilum | |
| 3.12.1.1 | N-ethylmaleimide | - |
Acidiphilium acidophilum | |
| 3.12.1.1 | tetrathionate | - |
Acidiphilium acidophilum | |
| 3.12.1.1 | thiosulfate | - |
Acidiphilium acidophilum | |
| 3.12.1.1 | trithionate | substrate inhibition, Ki: 6 mM | Acidiphilium acidophilum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.12.1.1 | 0.07 | - |
trithionate | at 30°C | Acidiphilium acidophilum | |
| 3.12.1.1 | 0.27 | - |
trithionate | at 70°C | Acidiphilium acidophilum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.12.1.1 | 34000 | - |
SDS-PAGE | Acidiphilium acidophilum |
| 3.12.1.1 | 99000 | - |
gel filtration | Acidiphilium acidophilum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.12.1.1 | trithionate + H2O | Acidiphilium acidophilum | involved in cytochrome c reduction | sulfate + thiosulfate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.12.1.1 | Acidiphilium acidophilum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.12.1.1 | - |
Acidiphilium acidophilum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.12.1.1 | trithionate + H2O | - |
Acidiphilium acidophilum | sulfate + thiosulfate | - |
? | |
| 3.12.1.1 | trithionate + H2O | involved in cytochrome c reduction | Acidiphilium acidophilum | sulfate + thiosulfate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.12.1.1 | tetramer | 4 * 34000 | Acidiphilium acidophilum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.12.1.1 | 70 | - |
- |
Acidiphilium acidophilum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.12.1.1 | 90 | - |
20% of maximum activity | Acidiphilium acidophilum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.12.1.1 | additional information | - |
no loss of activity by frequent freezing and thawing | Acidiphilium acidophilum |
| 3.12.1.1 | - |
- |
activity lost after 36 days | Acidiphilium acidophilum |
| 3.12.1.1 | 30 | - |
40% activity after 97 days | Acidiphilium acidophilum |
| 3.12.1.1 | 70 | - |
activity lost after 27 days | Acidiphilium acidophilum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.12.1.1 | 3.5 | 4.5 | - |
Acidiphilium acidophilum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.12.1.1 | 1 | 7.5 | active above pH 1 and below pH 7.5 | Acidiphilium acidophilum |
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Release 2023.1 (January 2023)
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