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Literature summary extracted from

  • Tholl, D.; Ober, D.; Martin, W.; Kellermann, J.; Hartmann, T.
    Purification, molecular cloning and expression in Escherichia coli of homospermidine synthase from Rhodopseudomonas viridis (1996), Eur. J. Biochem., 240, 373-379.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.44 overexpressed in Escherichia coli BL21, which originally does not possess HSS activity. 40-50% of the soluble protein in crude extracts are detected as homospermidine synthase Blastochloris viridis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.44 0.018
-
 NAD+
-
 Blastochloris viridis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.44 52000
-
 2 * 52000, SDS-PAGE Blastochloris viridis
2.5.1.44 52600
-
 2 * 52600, calculated from the amino acid sequence Blastochloris viridis
2.5.1.44 100000
-
 gel filtration Blastochloris viridis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.44 2 putrescine Blastochloris viridis
-
 sym-homospermidine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.44 Blastochloris viridis
-
 expressed in Escherichia coli strain BL21/pHsRvT72.2
-
2.5.1.44 Blastochloris viridis
-
 DSM 134
-
2.5.1.44 Blastochloris viridis
-
 accession no. L77975
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.44 224fold Blastochloris viridis
2.5.1.44 overexpressed Escherichia coli strain BL21 Blastochloris viridis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.44 culture medium
-
 Blastochloris viridis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.44 4.97
-
-
 Blastochloris viridis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.44 2 putrescine
-
 Blastochloris viridis sym-homospermidine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.44 dimer 2 * 52000, SDS-PAGE Blastochloris viridis
2.5.1.44 dimer 2 * 52600, calculated from the amino acid sequence Blastochloris viridis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.44 NAD+
-
 Blastochloris viridis