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Literature summary extracted from

  • Yamamoto, S.; Nagata, S.; Kusaba, K.
    Purification and characterization of homospermidine synthase in Acinetobacter tartarogenes ATCC 31105 (1993), J. Biochem., 114, 45-49.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.44 iodoacetamide preincubation, 1 mM, 30 min prior to assay causes 50% inhibition Acinetobacter tartarogenes
2.5.1.44 N-ethylmaleimide preincubation, 1 mM, 30 min prior to assay causes 91% inhibition Acinetobacter tartarogenes
2.5.1.44 NADH Ki: 0.006 mM Acinetobacter tartarogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.44 0.28
-
 putrescine
-
 Acinetobacter tartarogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.44 K+ required for full activity, optimal activity at 50 mM Acinetobacter tartarogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.44 52000
-
 2 * 52000, SDS-PAGE Acinetobacter tartarogenes
2.5.1.44 102000
-
 gel filtration Acinetobacter tartarogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.44 2 putrescine Acinetobacter tartarogenes
-
 sym-homospermidine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.44 Acinetobacter tartarogenes
-
 ATCC 31105
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.44
-
 Acinetobacter tartarogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.44 8.41
-
-
 Acinetobacter tartarogenes

Storage Stability

EC Number Storage Stability Organism
2.5.1.44 1°C, 20 mM, Tris/HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM putrescine, 0.02% NaN3 Acinetobacter tartarogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.44 2 putrescine
-
 Acinetobacter tartarogenes sym-homospermidine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.44 dimer 2 * 52000, SDS-PAGE Acinetobacter tartarogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.44 4
-
-
 Acinetobacter tartarogenes

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.44 NAD+
-
 Acinetobacter tartarogenes