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Literature summary extracted from

  • Uchida, Y.; Izai, K.; Orii, T.; Hashimoto, T.
    Novel fatty acid beta-oxidation enzymes in rat liver mitochondria. II. Purification and properties of enoyl-coenzyme A (CoA) hydratase/3-hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase trifunctional protein (1992), J. Biol. Chem., 267, 1034-1041.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.211 0.05
-
 NADH
-
 Rattus norvegicus
1.1.1.211 0.06
-
 NAD+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.211 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.1.16 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.1.155 glyoxysome
-
 Cucumis sativus 9514
-
2.3.1.155 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.211 51000
-
 alpha4beta4, 4 * 79000 + 4 * 51000, SDS-PAGE Rattus norvegicus
1.1.1.211 79000
-
 alpha4beta4, 4 * 79000 + 4 * 51000, SDS-PAGE Rattus norvegicus
1.1.1.211 460000
-
 gel filtration Rattus norvegicus
2.3.1.16 51000
-
 alpha4beta4, 4 * 79000 + 4 * 51000, SDS-PAGE Rattus norvegicus
2.3.1.16 79000
-
 alpha4beta4, 4 * 79000 + 4 * 51000, SDS-PAGE Rattus norvegicus
2.3.1.16 460000
-
 gel filtration Rattus norvegicus
2.3.1.16 460000
-
 multifunctional enzyme: 2-enoyl-CoA hydratase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA dehydrogenase, gel filtration Rattus norvegicus
2.3.1.155 41000
-
 2 * 41000, SDS-PAGE Rattus norvegicus
2.3.1.155 78000
-
 peroxisomal bifunctional protein, gel filtration Rattus norvegicus
2.3.1.155 89000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.16 CoA + 3-oxoacyl-CoA Rattus norvegicus
-
 acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxoacyl-CoA Rattus norvegicus Wistar
-
 acyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.211 Rattus norvegicus Q64428
-
-
2.3.1.16 Rattus norvegicus
-
-
-
2.3.1.16 Rattus norvegicus Q64428
-
-
2.3.1.155 Cucumis sativus
-
 cucumber
-
2.3.1.155 Escherichia coli
-
-
-
2.3.1.155 Homo sapiens
-
 human
-
2.3.1.155 Pseudomonas fragi
-
-
-
2.3.1.155 Rattus norvegicus
-
 Wistar
-
2.3.1.155 Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.211
-
 Rattus norvegicus
2.3.1.16
-
 Rattus norvegicus
2.3.1.155
-
 Rattus norvegicus
2.3.1.155
-
 Pseudomonas fragi

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.16 acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA multifunctional enzyme: 2-enoyl-CoA hydratase, 3-ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA dehydrogenase Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.211 liver
-
 Rattus norvegicus
-
2.3.1.16 liver
-
 Rattus norvegicus
-
2.3.1.155 liver
-
 Homo sapiens
-
2.3.1.155 liver
-
 Rattus norvegicus
-
2.3.1.155 seed
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.211 additional information
-
-
 Rattus norvegicus
2.3.1.16 additional information
-
-
 Rattus norvegicus
2.3.1.16 26.3
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.211 3-ketohexadecanoyl-CoA + NADH
-
 Rattus norvegicus 3-hydroxyhexadecanoyl-CoA + NAD+
-
 ?
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Rattus norvegicus acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Rattus norvegicus Wistar acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxooctanoyl-CoA
-
 Rattus norvegicus acetyl-CoA + hexanoyl-CoA
-
 ?
2.3.1.155 3-oxooctanoyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + hexanoyl-CoA
-
 ?
2.3.1.155 3-oxooctanoyl-CoA + CoA
-
 Rattus norvegicus Wistar acetyl-CoA + hexanoyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Escherichia coli acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Homo sapiens acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Cucumis sativus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Pseudomonas fragi acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Rattus norvegicus Wistar acetyl-CoA + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.211 octamer alpha4beta4, 4 * 79000 + 4 * 51000, SDS-PAGE Rattus norvegicus
2.3.1.16 octamer alpha4beta4, 4 * 79000 + 4 * 51000, SDS-PAGE Rattus norvegicus
2.3.1.155 dimer 2 * 41000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.211 enoyl-coenzyme A (CoA) hydratase/3-hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase trifunctional protein
-
 Rattus norvegicus
2.3.1.16 enoyl-coenzyme A (CoA) hydratase/3-hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase trifunctional protein
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.211 NADH
-
 Rattus norvegicus