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Literature summary extracted from

  • Shimizu, S.; Hattori, S.; Hata, H.; Yamada, H.
    A novel fungal enzyme, NADPH-dependent carbonyl reductase, showing high specificity to conjugated polyketones (1988), Eur. J. Biochem., 174, 37-44.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.6 1,3-cyclopentadione 1.0 mM Mucor ambiguus
1.6.5.6 3-methyl-1,2-cyclohexadione 1.0 mM Mucor ambiguus
1.6.5.6 5,5'-dithiobis(2-nitrobenzoic acid) 0.1 mM Mucor ambiguus
1.6.5.6 8-hydroxyquinoline 0.1 mM Mucor ambiguus
1.6.5.6 CdCl2 1.0 mM Mucor ambiguus
1.6.5.6 cyclohexadione 1.0 mM Mucor ambiguus
1.6.5.6 dicoumarol 0.1 mM Mucor ambiguus
1.6.5.6 FeCl2 1.0 mM Mucor ambiguus
1.6.5.6 additional information overview Mucor ambiguus
1.6.5.6 NADP+ 1.0 mM Mucor ambiguus
1.6.5.6 o-phenanthroline 1.0 mM Mucor ambiguus
1.6.5.6 quercetin 0.01 mM Mucor ambiguus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.6 0.0123
-
1-methylisatin
-
Mucor ambiguus
1.6.5.6 0.05
-
isatin
-
Mucor ambiguus
1.6.5.6 0.053
-
beta-naphthoquinone
-
Mucor ambiguus
1.6.5.6 0.135
-
NADPH
-
Mucor ambiguus
1.6.5.6 0.21
-
acenaphthenequinone
-
Mucor ambiguus
1.6.5.6 0.24
-
dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione
-
Mucor ambiguus
1.6.5.6 0.38
-
dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione
-
Mucor ambiguus
1.6.5.6 0.41
-
p-toluquinone
-
Mucor ambiguus
1.6.5.6 0.58
-
dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione
-
Mucor ambiguus
1.6.5.6 0.71
-
ketopantoyl lactone
-
Mucor ambiguus
1.6.5.6 1.61
-
dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione
-
Mucor ambiguus
1.6.5.6 2.5
-
alloxan
-
Mucor ambiguus
1.6.5.6 3.3
-
dihydro-4-methyl-4-propyl-2,3-furanedione
-
Mucor ambiguus
1.6.5.6 4.17
-
dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione
-
Mucor ambiguus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.6 27500
-
2 * 27500, SDS-PAGE Mucor ambiguus
1.6.5.6 44000
-
high-performance gel-permeation liquid chromatography, TSK G-3000 SW Mucor ambiguus
1.6.5.6 54000
-
gel filtration, Sephadex G-100 Mucor ambiguus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.6 Mucor ambiguus
-
-
-
1.6.5.6 Mucor ambiguus AKU 3006
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.6 purification to apparent homogenity Mucor ambiguus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.6 1-methylisatin + NADPH
-
Mucor ambiguus NADP+ + ?
-
ir
1.6.5.6 5-bromoisatin + NADPH
-
Mucor ambiguus NADP+ + ?
-
ir
1.6.5.6 5-methylisatin + NADPH
-
Mucor ambiguus NADP+ + ?
-
ir
1.6.5.6 acenaphthenequinone + NADPH reduced velocity Mucor ambiguus NADP+ + acenaphthylene-1,2-diol
-
ir
1.6.5.6 alloxan + NADPH
-
Mucor ambiguus NADP+ + ?
-
ir
1.6.5.6 alpha-naphthoquinone + NADPH
-
Mucor ambiguus NADP+ + naphthalene-1,4-diol
-
ir
1.6.5.6 alpha-naphthoquinone + NADPH
-
Mucor ambiguus AKU 3006 NADP+ + naphthalene-1,4-diol
-
ir
1.6.5.6 beta-naphthoquinone + NADPH
-
Mucor ambiguus NADP+ + naphthalene-1,2-diol
-
ir
1.6.5.6 beta-naphthoquinone + NADPH
-
Mucor ambiguus AKU 3006 NADP+ + naphthalene-1,2-diol
-
ir
1.6.5.6 dihydro-4-methyl-4-propyl-2,3-furanedione + NADPH
-
Mucor ambiguus NADP+ + 4-methyl-4-propyl-4,5-dihydrofuran-2,3-diol
-
ir
1.6.5.6 dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione + NADPH reduced velocity Mucor ambiguus NADP+ + 5-sec-butyl-4-ethyl-4-methyl-4,5-dihydrofuran-2,3-diol
-
ir
1.6.5.6 dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH
-
Mucor ambiguus NADP+ + 4,4-dimethyl-5-(1-methylbutyl)-4,5-dihydrofuran-2,3-diol
-
ir
1.6.5.6 dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione + NADPH reduced velocity Mucor ambiguus NADP+ + 4-methyl-5-(1-methylbutyl)-4-propyl-4,5-dihydrofuran-2,3-diol
-
ir
1.6.5.6 dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH
-
Mucor ambiguus NADP+ + 5-(1-ethylpropyl)-4,4-dimethyl-4,5-dihydrofuran-2,3-diol
-
ir
1.6.5.6 dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione + NADPH
-
Mucor ambiguus NADP+ + 5-isopropyl-4,4-dimethyl-4,5-dihydrofuran-2,3-diol
-
ir
1.6.5.6 isatin + NADPH
-
Mucor ambiguus NADP+ + 1-methyl-1H-indole-2,3-diol
-
ir
1.6.5.6 ketopantoyl lactone + NADPH
-
Mucor ambiguus NADP+ + ?
-
ir
1.6.5.6 ninhydrin + NADPH
-
Mucor ambiguus NADP+ + ?
-
ir
1.6.5.6 p-benzoquinone + NADPH
-
Mucor ambiguus NADP+ + hydroquinone
-
ir
1.6.5.6 p-benzoquinone + NADPH
-
Mucor ambiguus AKU 3006 NADP+ + hydroquinone
-
ir
1.6.5.6 p-toluquinone + NADPH + H+
-
Mucor ambiguus NADP+ + 2-methylbenzene-1,4-diol
-
ir
1.6.5.6 p-toluquinone + NADPH + H+
-
Mucor ambiguus AKU 3006 NADP+ + 2-methylbenzene-1,4-diol
-
ir
1.6.5.6 phenanthrenequinone + NADPH
-
Mucor ambiguus NADP+ + ?
-
ir
1.6.5.6 phenanthrenequinone + NADPH
-
Mucor ambiguus AKU 3006 NADP+ + ?
-
ir

Subunits

EC Number Subunits Comment Organism
1.6.5.6 dimer 2 * 27500, SDS-PAGE Mucor ambiguus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.5.6 40
-
reduction of ketopantoyl lactone Mucor ambiguus
1.6.5.6 45
-
reduction of isatin Mucor ambiguus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.6.5.6 15 50 for isatin and ketopantoyl lactone as substrates Mucor ambiguus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.5.6
-
40 15% of control activity at 50°C Mucor ambiguus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.6 6 7 for isatin reduction Mucor ambiguus
1.6.5.6 6
-
for ketopantoyl lactone reduction Mucor ambiguus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.5.6 5 9 with different buffers Mucor ambiguus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.6.5.6 6 7 91% of control activity at pH 9, 79% of control activity at pH 12, no activity at pH 4.0 Mucor ambiguus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.6 NADPH
-
Mucor ambiguus