EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.2.4 | expression in Escherichia coli | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.6.2.4 | Y140D | substitution does not eliminate FMN binding but reduces cytochrome c reductase activity, Km value for cytochrome c or NADPH similar to wild type | Rattus norvegicus |
1.6.2.4 | Y140D/178D | substitution abolishes FMN binding and cytochrome c reductase activity | Rattus norvegicus |
1.6.2.4 | Y140F | substitution has no effect on FMN content or catalytic activity, Km value for cytochrome c or NADPH similar to wild type | Rattus norvegicus |
1.6.2.4 | Y140F/178F | substitution has no effect on FMN content or catalytic activity, slightly decreases Km for cytochrome c, NADPH Km value slightly higher than wild type | Rattus norvegicus |
1.6.2.4 | Y178D | substitution abolishes FMN binding and cytochrome c reductase activity, Km value for cytochrome c similar to wild type, NADPH Km value slightly higher than wild type | Rattus norvegicus |
1.6.2.4 | Y178F | substitution has no effect on FMN content or catalytic activity, slightly decreases Km for cytochrome c, NADPH Km value similar to wild type | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.4 | 0.0064 | - |
NADPH | wild type, cosubstrate cytochrome c | Rattus norvegicus | |
1.6.2.4 | 0.0066 | - |
NADPH | native enzyme, liver, cosubstrate cytochrome c | Rattus norvegicus | |
1.6.2.4 | 0.0066 | - |
NADPH | Y140D mutant, cosubstrate cytochrome c | Rattus norvegicus | |
1.6.2.4 | 0.0071 | - |
NADPH | Y178F mutant, cosubstrate cytochrome c | Rattus norvegicus | |
1.6.2.4 | 0.0078 | - |
NADPH | Y140F mutant, cosubstrate cytochrome c | Rattus norvegicus | |
1.6.2.4 | 0.0095 | - |
NADPH | Y178D mutant, cosubstrate cytochrome c | Rattus norvegicus | |
1.6.2.4 | 0.0114 | - |
NADPH | Y140F/178F double mutant, cosubstrate cytochrome c | Rattus norvegicus | |
1.6.2.4 | 0.0131 | - |
cytochrome c | Y140F/178F double mutant | Rattus norvegicus | |
1.6.2.4 | 0.014 | - |
cytochrome c | Y178F mutant | Rattus norvegicus | |
1.6.2.4 | 0.0178 | - |
cytochrome c | native enzyme, liver | Rattus norvegicus | |
1.6.2.4 | 0.0185 | - |
cytochrome c | Y178D mutant | Rattus norvegicus | |
1.6.2.4 | 0.0188 | - |
cytochrome c | Y140F mutant | Rattus norvegicus | |
1.6.2.4 | 0.0191 | - |
cytochrome c | Y140D mutant | Rattus norvegicus | |
1.6.2.4 | 0.0211 | - |
cytochrome c | wild type | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 80000 | - |
wild type, x * 80000, SDS-PAGE | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.4 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.4 | expressed in Escherichia coli | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.2.4 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 0.28 | - |
Y140D/178D double mutant, electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 0.44 | - |
Y178D mutant, electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 11 | - |
Y140D mutant, electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 46 | - |
Y140F/178F double mutant, electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 47.4 | - |
Y178F mutant, electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 48.1 | - |
Y178D mutant, electron acceptor: ferricyanide | Rattus norvegicus |
1.6.2.4 | 51 | - |
Y140D/178D double mutant, electron acceptor: ferricyanide | Rattus norvegicus |
1.6.2.4 | 51.5 | - |
bacterially expressed reductase protein, electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 52.9 | - |
electron acceptor: ferricyanide | Rattus norvegicus |
1.6.2.4 | 53.3 | - |
electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 55.2 | - |
Y140F mutant, electron acceptor: cytochrome c | Rattus norvegicus |
1.6.2.4 | 94.2 | - |
Y140F/178F double mutant, electron acceptor: ferricyanide | Rattus norvegicus |
1.6.2.4 | 94.8 | - |
Y140F mutant, electron acceptor: ferricyanide | Rattus norvegicus |
1.6.2.4 | 102 | - |
bacterially expressed reductase protein, electron acceptor: ferricyanide | Rattus norvegicus |
1.6.2.4 | 102 | - |
Y140D mutant, electron acceptor: ferricyanide | Rattus norvegicus |
1.6.2.4 | 113 | - |
Y178F mutant, electron acceptor: ferricyanide | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Rattus norvegicus | 2 ferrocytochrome c + NADP+ + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.6.2.4 | ? | wild type, x * 80000, SDS-PAGE | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.4 | FAD | - |
Rattus norvegicus | |
1.6.2.4 | FMN | - |
Rattus norvegicus |