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Literature summary extracted from

  • Betz, G.; Roper, M.; Tsai, P.
    Steroid 17,20-lyase from testis microsomes: participation of NADPH cytochrome c reductase (1974), Arch. Biochem. Biophys., 163, 318-323.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Rattus norvegicus
-
 Sprague-Dawley strain
-
1.6.2.4 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 liver microsomes, column chromatography on Sephadex G-150 and hydroxylapatatite Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 liver phenobarbital-treated animals Rattus norvegicus
-
1.6.2.4 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.676
-
 purified enzyme, liver Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: cytochrome P450 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: cytochrome P450 Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 additional information component of the electron transport chain required for activity of the 17,20-lyase in testis microsomes, removal of 2-carbon side chain from 17-position of 21-carbon steroids Rattus norvegicus ?
-
 ?
1.6.2.4 additional information component of the electron transport chain required for activity of the 17,20-lyase in testis microsomes, removal of 2-carbon side chain from 17-position of 21-carbon steroids Rattus norvegicus Sprague-Dawley ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 50
-
 40% activity remaining after 3 min Rattus norvegicus