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Literature summary extracted from

  • Sakane, F.; Takahashi, K.; Koyama, J.
    Purification and characterization of a membrane-bound NADPH-cytochrome c reductase capable of catalyzing menadione-dependent O2- formation in guinea pig polymorphonuclear leukocytes (1984), J. Biochem., 96, 671-678.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 2,6-dichlorophenolindophenol formation of superoxide anion, in the presence of menadione Cavia porcellus
1.6.2.4 EDTA reduction of cytochrome c and formation of superoxide anion Cavia porcellus
1.6.2.4 N-ethylmaleimide reduction of cytochrome c and formation of superoxide anion Cavia porcellus
1.6.2.4 quercetin reduction of cytochrome c and formation of superoxide anion Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 additional information
-
 additional information O2-generation Cavia porcellus
1.6.2.4 0.0053
-
 menadione
-
 Cavia porcellus
1.6.2.4 0.025
-
 NADPH
-
 Cavia porcellus
1.6.2.4 0.23
-
 NADH
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 plasma membrane
-
 Cavia porcellus 5886
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 solubilization with 0.2% Triton X-100, gel filtration on Sephacryl S-300 and affinity chromatography on 2',5'-ADP agarose Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.68
-
-
 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: nitroblue tetrazolium salt Cavia porcellus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: menadione Cavia porcellus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Cavia porcellus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 NADH + H+ + ferricytochrome c
-
 Cavia porcellus NAD+ + ferrocytochrome c
-
 r
1.6.2.4 NADPH + H+ + menadione
-
 Cavia porcellus NADP+ + menadiol
-
 r
1.6.2.4 NADPH + O2 slow reaction, presence of menadione, or duroquinone, or vitamin K3 essential Cavia porcellus NADP+ + O2- replacement of NADPH by NADH lowers the O2- generation ?
1.6.2.4 NADPH + O2 slow reaction, presence of menadione, or duroquinone, or vitamin K3 essential Cavia porcellus NADP+ + O2- superoxide anion ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 100
-
 10 min, inhibition of O2-formation Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7 7.4 O2- generation Cavia porcellus