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Literature summary extracted from
- Purification of a flavoprotein having NADPH-cytochrome c reductase and transhydrogenase activities from Nitrobacter winogradskyi and its molecular and enzymatic properties (1987), Arch. Microbiol., 148, 95-99.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | spinach ferredoxin | stimulation of the NADPH-reduction of cytochrome c-550 and horse cytochrome c | Nitrobacter winogradskyi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | NAD+ | cosubstrate NADH | Nitrobacter winogradskyi |  |
| 1.6.2.4 | NADP+ | cosubstrate NADPH | Nitrobacter winogradskyi | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.4 | 0.00189 | - |
NADPH | - |
Nitrobacter winogradskyi | |
| 1.6.2.4 | 1.4 | - |
4-Nitroacetophenone | electron donor NADH, electron acceptor dichlorophenolindophenol | Nitrobacter winogradskyi | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.2.4 | cytosol | - |
Nitrobacter winogradskyi | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | 36000 | - |
2 * 36000, SDS-PAGE | Nitrobacter winogradskyi |
| 1.6.2.4 | 70000 | - |
gel filtration | Nitrobacter winogradskyi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.1.4 | Nitrobacter winogradskyi | - |
- |
- |
| 1.6.1.4 | Nitrobacter winogradskyi ATCC 14123 | - |
- |
- |
| 1.6.2.4 | Nitrobacter winogradskyi | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | - |
Nitrobacter winogradskyi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | 8.64 | - |
electron acceptor: dichlorophenolindophenol | Nitrobacter winogradskyi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.1.4 | additional information | flavoenzyme, additionally shows NADPH-cytochrome c reductase activity, EC 1.6.2.4 | Nitrobacter winogradskyi | ? | - |
? | |
| 1.6.1.4 | NADH + H+ + 2 NADP+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
Nitrobacter winogradskyi | NAD+ + 2 NADPH + 2 oxidized ferredoxin [iron-sulfur] cluster | 0.064 mol of NAD + is reduced per mol of enzyme per s with NADPH produced continuously by glucose-6-phosphate dehydrogenase system | ? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: duroquinone | Nitrobacter winogradskyi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: benzylviologen | Nitrobacter winogradskyi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: menadione | Nitrobacter winogradskyi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Nitrobacter winogradskyi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: duroquinone | Nitrobacter winogradskyi ATCC 14123 | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: benzylviologen | Nitrobacter winogradskyi ATCC 14123 | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: menadione | Nitrobacter winogradskyi ATCC 14123 | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Nitrobacter winogradskyi ATCC 14123 | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | 4-nitroacetophenone + NADPH + H+ | - |
Nitrobacter winogradskyi | ? | - |
r | |
| 1.6.2.4 | 4-nitroacetophenone + NADPH + H+ | - |
Nitrobacter winogradskyi ATCC 14123 | ? | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | dimer | 2 * 36000, SDS-PAGE | Nitrobacter winogradskyi |
| 1.6.2.4 | More | N-terminal amino acid sequence is 27% homologous to that of the Spirulina ferredoxin-NADP reductase | Nitrobacter winogradskyi |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.4 | additional information | - |
additional information | - |
Nitrobacter winogradskyi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.4 | FAD | - |
Nitrobacter winogradskyi | |
| 1.6.2.4 | FAD | 1 mol per subunit | Nitrobacter winogradskyi | |
| 1.6.2.4 | FMN | 0.87 nmol per mg protein | Nitrobacter winogradskyi | |
| 1.6.2.4 | NADH | less effective than NADPH | Nitrobacter winogradskyi | |
| 1.6.2.4 | NADPH | - |
Nitrobacter winogradskyi | |
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