EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.98.2 | 0.003 | - |
reduced coenzyme F420 | - |
Methanothermobacter thermautotrophicus | |
1.5.98.2 | 0.3 | - |
N5,N10-methylenetetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.98.2 | 36000 | - |
4 * 36000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.5.98.2 | 38000 | - |
8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.5.98.2 | 150000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
1.5.98.2 | 300000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.98.2 | N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | Methanothermobacter thermautotrophicus | - |
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r | |
1.5.98.2 | N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.98.2 | Methanothermobacter thermautotrophicus | - |
- |
- |
1.5.98.2 | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.98.2 | - |
Methanothermobacter thermautotrophicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.98.2 | 163 | - |
- |
Methanothermobacter thermautotrophicus |
1.5.98.2 | 290 | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.98.2 | N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | - |
Methanothermobacter thermautotrophicus | 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r | |
1.5.98.2 | N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420 | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.98.2 | octamer | 8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
1.5.98.2 | tetramer | 4 * 36000, SDS-PAGE | Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.98.2 | 65 | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.98.2 | 90 | - |
inactivation after a few min, increased thermostability when salts are added | Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.98.2 | 6.5 | 7 | - |
Methanothermobacter thermautotrophicus |
1.5.98.2 | 7 | - |
- |
Methanothermobacter thermautotrophicus |