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Literature summary extracted from

  • Ma, K.; Thauer, R.K.
    Purification and properties of N5,N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg) (1990), Eur. J. Biochem., 191, 187-193.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.2 0.003
-
reduced coenzyme F420
-
Methanothermobacter thermautotrophicus
1.5.98.2 0.3
-
N5,N10-methylenetetrahydromethanopterin
-
Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.2 36000
-
4 * 36000, SDS-PAGE Methanothermobacter thermautotrophicus
1.5.98.2 38000
-
8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus
1.5.98.2 150000
-
gel filtration Methanothermobacter thermautotrophicus
1.5.98.2 300000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.98.2 N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 Methanothermobacter thermautotrophicus
-
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r
1.5.98.2 N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.2 Methanothermobacter thermautotrophicus
-
-
-
1.5.98.2 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.2
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.2 163
-
-
Methanothermobacter thermautotrophicus
1.5.98.2 290
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.2 N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420
-
Methanothermobacter thermautotrophicus 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r
1.5.98.2 N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r

Subunits

EC Number Subunits Comment Organism
1.5.98.2 octamer 8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus
1.5.98.2 tetramer 4 * 36000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.2 65
-
-
Methanothermobacter thermautotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.98.2 90
-
inactivation after a few min, increased thermostability when salts are added Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.2 6.5 7
-
Methanothermobacter thermautotrophicus
1.5.98.2 7
-
-
Methanothermobacter thermautotrophicus