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Literature summary extracted from

  • Romanov, V.; Hausinger, R.P.
    NADPH-dependent reductive ortho dehalogenation of 2,4-dichlorobenzoic acid in Corynebacterium sepedonicum KZ-4 and Coryneform Bacterium strain NBT-1 via 2,4-dichlorobenzoyl coenzym A (1996), J. Bacteriol., 178, 2656-2661.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.1.2 dithiothreitol accumulation of 13% 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
1.21.1.2 glutathione accumulation of 14% 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
1.21.1.2 MgATP2- conversation of 2,4-dichlorobenzoyl-CoA in cytosolic fraction Clavibacter michiganensis subsp. sepedonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH Clavibacter michiganensis subsp. sepedonicus
-
4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH Coryneform bacterium pathway for 2,4-dichlorobenzoate catabolism 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH Coryneform bacterium NTB-1 pathway for 2,4-dichlorobenzoate catabolism 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH Clavibacter michiganensis subsp. sepedonicus KZ-4
-
4-chlorobenzoyl-CoA + NADP+ + chloride
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.21.1.2 Clavibacter michiganensis subsp. sepedonicus
-
-
-
1.21.1.2 Clavibacter michiganensis subsp. sepedonicus KZ-4
-
-
-
1.21.1.2 Coryneform bacterium
-
formerly named Alcaligenes denitrificans
-
1.21.1.2 Coryneform bacterium NTB-1
-
formerly named Alcaligenes denitrificans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.1.2 0.0008
-
-
Clavibacter michiganensis subsp. sepedonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH
-
Clavibacter michiganensis subsp. sepedonicus 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH
-
Coryneform bacterium 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH pathway for 2,4-dichlorobenzoate catabolism Coryneform bacterium 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH
-
Coryneform bacterium NTB-1 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH pathway for 2,4-dichlorobenzoate catabolism Coryneform bacterium NTB-1 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?
1.21.1.2 2,4-dichlorobenzoyl-CoA + NADPH
-
Clavibacter michiganensis subsp. sepedonicus KZ-4 4-chlorobenzoyl-CoA + NADP+ + chloride
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.1.2 CoA conversion of 2,4-dichlorobenzoyl-CoA in cytosolic fraction Clavibacter michiganensis subsp. sepedonicus
1.21.1.2 NADH 10% accumulation of 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
1.21.1.2 NADH 2% accumulation of 4-chlorobenzoyl-CoA in cell extract Coryneform bacterium
1.21.1.2 NADPH 100% accumulation of 4-chlorobenzoyl-CoA in cell extract Clavibacter michiganensis subsp. sepedonicus
1.21.1.2 NADPH 100% accumulation of 4-chlorobenzoyl-CoA in cell extract Coryneform bacterium