EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.2.B1 | Cu2+ | partial inhibition | Methylococcus capsulatus | |
1.2.2.B1 | Fe2+ | partial inhibition | Methylococcus capsulatus | |
1.2.2.B1 | additional information | the enzyme is insensitive to EDTA, N-ethylmaleimide, cyanide and azide | Methylococcus capsulatus | |
1.2.2.B1 | phenylhydrazine | - |
Methylococcus capsulatus | |
1.2.2.B1 | Zn2+ | partial inhibition | Methylococcus capsulatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.2.B1 | 0.143 | - |
formaldehyde | 20°C, pH 7.4 | Methylococcus capsulatus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.2.B1 | membrane | associated | Methylococcus capsulatus | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.2.B1 | 49500 | - |
4 * 49500, SDS-PAGE | Methylococcus capsulatus |
1.2.2.B1 | 210000 | - |
gel filtration | Methylococcus capsulatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 complex | Methylococcus capsulatus | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | formate + ferrocytochrome b559/569 complex | - |
? | |
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 complex | Methylococcus capsulatus Bath | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | formate + ferrocytochrome b559/569 complex | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.2.B1 | Methylococcus capsulatus | - |
- |
- |
1.2.2.B1 | Methylococcus capsulatus Bath | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.2.B1 | - |
Methylococcus capsulatus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.2.B1 | culture condition:high copper medium-grown cell | - |
Methylococcus capsulatus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.2.B1 | 146 | - |
- |
Methylococcus capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.2.B1 | formaldehyde + dichloroindophenol | formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol | Methylococcus capsulatus | formate + reduced dichloroindophenol | - |
? | |
1.2.2.B1 | formaldehyde + dichloroindophenol | formaldehyde is the only substrate that supports enzyme-mediated reduction of dichloroindophenol | Methylococcus capsulatus Bath | formate + reduced dichloroindophenol | - |
? | |
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 + H2O | - |
Methylococcus capsulatus | formate + ferrocytochrome b559/569 + 2 H+ | - |
r | |
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 + H2O | - |
Methylococcus capsulatus Bath | formate + ferrocytochrome b559/569 + 2 H+ | - |
r | |
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 complex | - |
Methylococcus capsulatus | formate + ferrocytochrome b559/569 complex | - |
? | |
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 complex | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | Methylococcus capsulatus | formate + ferrocytochrome b559/569 complex | - |
? | |
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 complex | - |
Methylococcus capsulatus Bath | formate + ferrocytochrome b559/569 complex | - |
? | |
1.2.2.B1 | formaldehyde + ferricytochrome b559/569 complex | the enzyme is involved in the pathways of methane oxidation. Cytochrome b559/569 complex is the physiological electron acceptor. The enzyme is the major formaldehyde-oxidizing enzyme in cells cultured in high (above 1 mol of Cu per mg of cell protein) copper medium | Methylococcus capsulatus Bath | formate + ferrocytochrome b559/569 complex | - |
? | |
1.2.2.B1 | additional information | no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde | Methylococcus capsulatus | ? | - |
? | |
1.2.2.B1 | additional information | no activity with: methanol, acetaldehyde, glyoxal, or butyraldehyde | Methylococcus capsulatus Bath | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.2.B1 | homotetramer | 4 * 49500, SDS-PAGE | Methylococcus capsulatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.2.B1 | DL-FalDH | - |
Methylococcus capsulatus |
1.2.2.B1 | formaldehyde-cytochrome b559/569 oxidoreductase | - |
Methylococcus capsulatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.2.B1 | 266 | - |
ferricytochrome b559/569 | 20°C, pH 7.4 | Methylococcus capsulatus | |
1.2.2.B1 | 290 | - |
dichloroindophenol | 20°C, pH 7.4 | Methylococcus capsulatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.2.B1 | 7.4 | - |
- |
Methylococcus capsulatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.2.B1 | pyrroloquinoline quinone | pyrroloquinoline quinone-to-subunit stochiometry of approximately 1:1, 3.6 mol of pyrroloquinoline quinone per mol of enzyme | Methylococcus capsulatus |