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Literature summary extracted from

  • Gehring, U.; Arnon, D.I.
    Purification and properties of alpha-ketoglutarate synthase from a photosynthetic bacterium (1972), J. Biol. Chem., 247, 6963-6969.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.7.3 stabilized under anaerobic conditions by a combination of dimercaptoethanol and FeSO4 Prosthecochloris vibrioformis f. thiosulfatophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.3 0.25
-
 succinyl-CoA pH and temperature not specified in the publication Prosthecochloris vibrioformis f. thiosulfatophilum
1.2.7.3 2.6
-
 2-oxoglutarate pH and temperature not specified in the publication Prosthecochloris vibrioformis f. thiosulfatophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.3 additional information no effect of Mg2+ Prosthecochloris vibrioformis f. thiosulfatophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.3 220000
-
 sucrose density gradient sedimentation Prosthecochloris vibrioformis f. thiosulfatophilum

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.3 Prosthecochloris vibrioformis f. thiosulfatophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.3
-
 Prosthecochloris vibrioformis f. thiosulfatophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.3 1.25
-
 pH and temperature not specified in the publication Prosthecochloris vibrioformis f. thiosulfatophilum

Storage Stability

EC Number Storage Stability Organism
1.2.7.3 0°C, anaerobic conditions, 2,3-dimercaptoethanol plus FeSO4 Prosthecochloris vibrioformis f. thiosulfatophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.3 succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ of several ferredoxins tested, only those from the photosynthetic bacteria Chlorobium thiosulfatophilum, Chromatium, and Rhodospirillum rubrum are effective. The ferredoxins of Clostridium pasteurianum and spinach are poor substitutes for those of photosynthetic bacteria Prosthecochloris vibrioformis f. thiosulfatophilum 2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.7.3 alpha-ketoglutarate synthase
-
 Prosthecochloris vibrioformis f. thiosulfatophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.3 7.5 7.8 optimum with a sharp drop on the acidic side Prosthecochloris vibrioformis f. thiosulfatophilum