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Literature summary extracted from

  • Akamatsu, Y.; Shimada, M.
    Partial purification and characterization of glyoxylate oxidase from the brown-rot basidiomycete Tyromyces palustris (1994), Phytochemistry, 37, 649-653.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.5 potassium ferricyanide 1 mM concentration 158% activation Tyromyces palustris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.5 4,4-dihydroxy-1,3-benzene disulfonic acid disodium salt 2 mM concentration 17% inhibition Tyromyces palustris
1.2.3.5 benzoquinone 0.01 mM concentration 100% inhibition Tyromyces palustris
1.2.3.5 CuSO4 0.05 mM concentration 100% inhibition Tyromyces palustris
1.2.3.5 fluoroacetate 2 mM concentration 22% inhibition Tyromyces palustris
1.2.3.5 oxalate moderate inhibitor Tyromyces palustris
1.2.3.5 p-chloromercuribenzoate 0.1 mM concentration 100% inhibition Tyromyces palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.5 3.7
-
 glyoxylate
-
 Tyromyces palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.5 127000
-
 gel filtration Tyromyces palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.3.5 glyoxylic acid + O2 + H2O Tyromyces palustris
-
 oxalate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.5 Tyromyces palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.5
-
 Tyromyces palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.5 glycolaldehyde + O2 + H2O
-
 Tyromyces palustris ?
-
 ?
1.2.3.5 glycolate + O2 + H2O
-
 Tyromyces palustris ?
-
 ?
1.2.3.5 glyoxylic acid + O2 + H2O
-
 Tyromyces palustris oxalate + H2O2
-
 ?
1.2.3.5 glyoxylic acid + O2 + H2O 2,6-dichlorophenolindophenol can also act as electron-acceptor Tyromyces palustris oxalate + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.5 40
-
-
 Tyromyces palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.5 8
-
-
 Tyromyces palustris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.3.5 6.5 8.5 at pH 6.5 and 8.5 50% activity Tyromyces palustris

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.3.5 additional information independent of NAD+ and NADP+ Tyromyces palustris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.3.5 0.05
-
 oxalate
-
 Tyromyces palustris