BRENDA - Enzyme Database show

Purification and properties of oxalic acid oxidase

Chiriboga, J.; Arch. Biochem. Biophys. 116, 516-523 (1966)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.3.4
8-hydroxyquinoline
0.4 mM concentration 400% activation
Hordeum vulgare
1.2.3.4
arsenite
5 mM concentration slight activation
Hordeum vulgare
1.2.3.4
cyanide
5 mM concentration slight activation
Hordeum vulgare
1.2.3.4
FAD
0.001 mM concentration
Hordeum vulgare
1.2.3.4
FMN
0.001 mM concentration
Hordeum vulgare
1.2.3.4
riboflavin
0.001 mM concentration
Hordeum vulgare
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.4
arsenite
concentration higher than 5 mM
Hordeum vulgare
1.2.3.4
cyanide
50 mM concentration
Hordeum vulgare
1.2.3.4
fluoride
0.5 mM concentration
Hordeum vulgare
1.2.3.4
iodoacetate
0.5 mM concentration
Hordeum vulgare
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.4
0.42
-
oxalic acid
-
Hordeum vulgare
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.3.4
cytosol
-
Hordeum vulgare
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.3.4
Hordeum vulgare
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.3.4
-
Hordeum vulgare
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.3.4
2.98
-
oxalic acid consumption
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.4
oxalic acid + O2 + 2 H+
-
288394
Hordeum vulgare
2 CO2 + H2O2
-
-
-
ir
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.3.4
3.5
-
-
Hordeum vulgare
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.3.4
8-hydroxyquinoline
0.4 mM concentration 400% activation
Hordeum vulgare
1.2.3.4
arsenite
5 mM concentration slight activation
Hordeum vulgare
1.2.3.4
cyanide
5 mM concentration slight activation
Hordeum vulgare
1.2.3.4
FAD
0.001 mM concentration
Hordeum vulgare
1.2.3.4
FMN
0.001 mM concentration
Hordeum vulgare
1.2.3.4
riboflavin
0.001 mM concentration
Hordeum vulgare
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.4
arsenite
concentration higher than 5 mM
Hordeum vulgare
1.2.3.4
cyanide
50 mM concentration
Hordeum vulgare
1.2.3.4
fluoride
0.5 mM concentration
Hordeum vulgare
1.2.3.4
iodoacetate
0.5 mM concentration
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.4
0.42
-
oxalic acid
-
Hordeum vulgare
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.3.4
cytosol
-
Hordeum vulgare
5829
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.3.4
-
Hordeum vulgare
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.3.4
2.98
-
oxalic acid consumption
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.4
oxalic acid + O2 + 2 H+
-
288394
Hordeum vulgare
2 CO2 + H2O2
-
-
-
ir
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.3.4
3.5
-
-
Hordeum vulgare