EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.306 | factor | unidentified factor that is required for maximal activity with formaldehyde as substrate, only 4% activity in its absence, oxidation of alcohols do not require the factor | Amycolatopsis methanolica | |
1.1.1.306 | methanol | can replace an unidentified activating factor | Amycolatopsis methanolica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.192 | 1,10-phenanthroline | - |
Amycolatopsis methanolica | |
1.1.1.192 | CuCl2 | - |
Amycolatopsis methanolica | |
1.1.1.192 | HgCl2 | - |
Amycolatopsis methanolica | |
1.1.1.192 | KCN | - |
Amycolatopsis methanolica | |
1.1.1.192 | additional information | inhibition differs for ethanol and formaldehyde as substrates | Amycolatopsis methanolica | |
1.1.1.306 | 1,10-phenanthroline | 80% inhibition at 5 mM | Amycolatopsis methanolica | |
1.1.1.306 | 2-(cyclohexylamino)ethanesulfonic acid-NaOH | 25 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica | |
1.1.1.306 | acetaldehyde | 15% inhibition at 1 mM | Amycolatopsis methanolica | |
1.1.1.306 | Cu2+ | complete inhibition at 1 mM | Amycolatopsis methanolica | |
1.1.1.306 | glycine-NaOH | 100 mM at pH 9.0: 40% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica | |
1.1.1.306 | Hg2+ | complete inhibition at 0.1 mM | Amycolatopsis methanolica | |
1.1.1.306 | KCN | 55% inhibition at 2 mM | Amycolatopsis methanolica | |
1.1.1.306 | Na2B4O7-HCl | 100 mM at pH 9.0: no activity | Amycolatopsis methanolica | |
1.1.1.306 | Tris-HCl | 50 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.192 | 1.2 | - |
1-Octanol | - |
Amycolatopsis methanolica | |
1.1.1.192 | 1.7 | - |
12-Hydroxydodecanoic acid | - |
Amycolatopsis methanolica | |
1.1.1.192 | 3.4 | - |
1-Hexanol | - |
Amycolatopsis methanolica | |
1.1.1.192 | 26.5 | - |
1-Pentanol | - |
Amycolatopsis methanolica | |
1.1.1.192 | 84 | - |
1-butanol | - |
Amycolatopsis methanolica | |
1.1.1.192 | 324 | - |
1-propanol | - |
Amycolatopsis methanolica | |
1.1.1.192 | 343 | - |
ethanol | - |
Amycolatopsis methanolica | |
1.1.1.306 | 1.2 | - |
1-Octanol | - |
Amycolatopsis methanolica | |
1.1.1.306 | 1.7 | - |
12-Hydroxydodecanoic acid | - |
Amycolatopsis methanolica | |
1.1.1.306 | 3.4 | - |
1-Hexanol | - |
Amycolatopsis methanolica | |
1.1.1.306 | 9.6 | - |
formaldehyde | - |
Amycolatopsis methanolica | |
1.1.1.306 | 26.5 | - |
1-Pentanol | - |
Amycolatopsis methanolica | |
1.1.1.306 | 84 | - |
1-butanol | - |
Amycolatopsis methanolica | |
1.1.1.306 | 324 | - |
1-propanol | - |
Amycolatopsis methanolica | |
1.1.1.306 | 343 | - |
ethanol | - |
Amycolatopsis methanolica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.192 | Zinc | 6.1 atoms/enzyme molecule | Amycolatopsis methanolica | |
1.1.1.306 | Zn2+ | 6 atoms Zn/enzyme molecule | Amycolatopsis methanolica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.192 | 40000 | - |
3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
1.1.1.192 | 117000 | 120000 | PAGE, gel filtration | Amycolatopsis methanolica |
1.1.1.306 | 40000 | - |
3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
1.1.1.306 | 120000 | - |
gel filtration | Amycolatopsis methanolica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.192 | long-chain alcohol + NAD+ + H2O | Amycolatopsis methanolica | enzyme also uses formaldehyde and short chain aliphatic alcohols | long-chain carboxylate + NADH | - |
? | |
1.1.1.306 | formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+ | Amycolatopsis methanolica | - |
S-formylmycothiol + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.192 | Amycolatopsis methanolica | - |
- |
- |
1.1.1.306 | Amycolatopsis methanolica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.306 | to homogeneity | Amycolatopsis methanolica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.306 | cell culture | induction of enzyme and factor synthesis only if cells grown in methanol-containing medium | Amycolatopsis methanolica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.306 | 0.03 | - |
cell free extract, in the absence of an unknown activating factor | Amycolatopsis methanolica |
1.1.1.306 | 0.08 | - |
cell free extract, in the presence of an unknown activating factor | Amycolatopsis methanolica |
1.1.1.306 | 6.9 | - |
substrate: ethanol | Amycolatopsis methanolica |
1.1.1.306 | 15 | - |
substrate: formaldehyde | Amycolatopsis methanolica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.192 | 1-octanol + 2 NAD+ + H2O | - |
Amycolatopsis methanolica | octanoic acid + 2 NADH + 2 H+ | - |
r | |
1.1.1.192 | 12-hydroxydodecanoic acid + NAD+ + H2O | - |
Amycolatopsis methanolica | ? + NADH | - |
? | |
1.1.1.192 | ethanol + NAD+ + H2O | - |
Amycolatopsis methanolica | ethanal + NADH | - |
? | |
1.1.1.192 | long-chain alcohol + NAD+ + H2O | enzyme also uses formaldehyde and short chain aliphatic alcohols | Amycolatopsis methanolica | long-chain carboxylate + NADH | - |
? | |
1.1.1.192 | n-butanol + NAD+ + H2O | - |
Amycolatopsis methanolica | n-butanal + NADH | - |
? | |
1.1.1.192 | n-hexanol + NAD+ + H2O | - |
Amycolatopsis methanolica | n-hexanal + NADH | - |
? | |
1.1.1.192 | n-pentanol + NAD+ + H2O | - |
Amycolatopsis methanolica | n-pentanal + NADH | - |
? | |
1.1.1.192 | n-propanol + NAD+ + H2O | - |
Amycolatopsis methanolica | n-propanal + NADH | - |
? | |
1.1.1.306 | 1-butanol + NAD+ | - |
Amycolatopsis methanolica | n-butanal + NADH | - |
? | |
1.1.1.306 | 1-hexanol + NAD+ | - |
Amycolatopsis methanolica | hexanal + NADH + H+ | - |
r | |
1.1.1.306 | 1-octanol + NAD+ | - |
Amycolatopsis methanolica | octanal + NADH + H+ | - |
r | |
1.1.1.306 | 1-pentanol + NAD+ | - |
Amycolatopsis methanolica | pentanal + NADH + H+ | - |
r | |
1.1.1.306 | 1-propanol + NAD+ | - |
Amycolatopsis methanolica | propanal + NADH + H+ | - |
r | |
1.1.1.306 | 12-hydroxydodecanoic acid + NADH | - |
Amycolatopsis methanolica | ? + NAD+ | - |
? | |
1.1.1.306 | ethanol + NAD+ | - |
Amycolatopsis methanolica | ethanal + NADH + H+ | - |
r | |
1.1.1.306 | formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+ | - |
Amycolatopsis methanolica | S-formylmycothiol + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.192 | More | N-terminal sequence | Amycolatopsis methanolica |
1.1.1.192 | trimer | 3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
1.1.1.306 | trimer | 3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.306 | FD-FA1DH | - |
Amycolatopsis methanolica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.306 | 9 | - |
formaldehyde as substrate | Amycolatopsis methanolica |
1.1.1.306 | 10.2 | - |
alcohols as substrates | Amycolatopsis methanolica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.306 | NAD+ | - |
Amycolatopsis methanolica |