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Literature summary extracted from

  • Matsuda, H.; Suzuki, Y.
    gamma-Guanidinobutyraldehyde dehydrogenase of Vicia faba leaves (1984), Plant Physiol., 76, 654-657.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.54 2-Carboxy-2'-hydroxy-5'-sulfoformazylbenzene zincon Vicia faba
1.2.1.54 diethyldithiocarbamate
-
 Vicia faba
1.2.1.54 Ferron
-
 Vicia faba
1.2.1.54 N-ethylmaleimide
-
 Vicia faba
1.2.1.54 o-phenanthroline
-
 Vicia faba
1.2.1.54 p-chloromercuribenzoate
-
 Vicia faba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.54 0.0034
-
 gamma-guanidinobutyraldehyde
-
 Vicia faba
1.2.1.54 0.025
-
 gamma-aminobutyraldehyde
-
 Vicia faba
1.2.1.54 0.084
-
 NAD+ cosubstrate gamma-guanidinobutyraldehyde Vicia faba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.54 83000
-
 gel filtration Vicia faba

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.54 Vicia faba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.54
-
 Vicia faba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.54 leaf
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.54 49.73
-
-
 Vicia faba

Storage Stability

EC Number Storage Stability Organism
1.2.1.54 1-13 days, 4°C, 25-90% loss of activity, partial reactivation by dithiothreitol and 2-mercaptoethanol Vicia faba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.54 4-aminobutyraldehyde + NAD+
-
 Vicia faba 4-aminobutanoate + NADH
-
 ?
1.2.1.54 4-guanidinobutanal + NAD+
-
 Vicia faba 4-guanidinobutanoate + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.54 45
-
-
 Vicia faba

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.54
-
 20 stable Vicia faba
1.2.1.54 30
-
 10 min, 4% loss of activity Vicia faba
1.2.1.54 50
-
 10 min, 62% loss of activity Vicia faba
1.2.1.54 60
-
 rapidly inactivated above Vicia faba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.54 9.5
-
-
 Vicia faba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.54 7.5 10 pH 7.5: about 20% of activity maximum, pH 10: about 70% of activity maximum Vicia faba

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.54 5.7 8.6 highest stability, 0°C, 24 h, 25% loss of activity Vicia faba
1.2.1.54 9
-
 0°C, 24 h, 63% loss of activity Vicia faba
1.2.1.54 10
-
 0°C, 24 h, 96% loss of activity Vicia faba

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.54 additional information not NADP+ Vicia faba
1.2.1.54 NAD+
-
 Vicia faba