EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.49 | D,L-2-amino-1-propanol | activation of aldehyde oxidation | Rattus norvegicus | |
1.2.1.49 | L-serine methyl ester | activation of aldehyde oxidation | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.49 | 0.14 | - |
methylglyoxal | - |
Rattus norvegicus | |
1.2.1.49 | 0.21 | 0.83 | methylglyoxal | - |
Rattus norvegicus | |
1.2.1.49 | 0.8 | - |
NADP+ | - |
Rattus norvegicus | |
1.2.1.49 | 8 | - |
methylglyoxal | amine as activator in aldehyde oxidation | Rattus norvegicus | |
1.2.1.49 | 8 | - |
methylglyoxal | in the presence of L-serine methyl ester (Km 8.0 mM) | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.49 | 50000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.49 | methylglyoxal + NADP+ + H2O | Rattus norvegicus | ir | pyruvate + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.49 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.49 | using ammonium sulfate fractionation and chromatography on DEAE-Sephadex column, Blue-dextran affinity column and CM-Sephadex column | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.49 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.49 | additional information | - |
- |
Rattus norvegicus |
1.2.1.49 | 7.25 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.49 | -20°C, 50% glycerol | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.49 | methylglyoxal + NADP+ + H2O | ir | Rattus norvegicus | pyruvate + NADPH + H+ | - |
? | |
1.2.1.49 | additional information | the only known amine which activates the enzyme at physiological pH is L-serine methyl ester, L-2-amino-1-propanol gives very little activity but D,L-2-amino-1-propanol activates the enzyme | Rattus norvegicus | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.49 | 8.5 | - |
- |
Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.49 | 9.3 | - |
activity falls abruptly above | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.49 | 9.3 | - |
not stable above | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.49 | NADP+ | - |
Rattus norvegicus |